Application of CHKA proteins in preparing kit for prognostic evaluation of liver cancer, kit and method

A technology of liver cancer prognosis and kit, applied in the biological field

Inactive Publication Date: 2017-01-04
王红阳 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, many studies have shown that choline kinase and its catalytic product phosphorylcholine are highly expressed in various tumors including breast cancer, lung cancer, colon cancer, bladder cancer, prostate cancer and ovarian cancer, and found that The expre

Method used

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  • Application of CHKA proteins in preparing kit for prognostic evaluation of liver cancer, kit and method
  • Application of CHKA proteins in preparing kit for prognostic evaluation of liver cancer, kit and method
  • Application of CHKA proteins in preparing kit for prognostic evaluation of liver cancer, kit and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Differential expression of CHKA protein in cancer tissues and adjacent tissues

[0039] 1. Western-blot detection of CHKA expression in liver cancer and adjacent tissues

[0040] 42 cases were randomly selected from 120 liver cancer cases, that is, 120 liver cancer tissue samples, and the expression of CHKA protein in cancer tissues and adjacent tissues was detected by Western-blot technology with CHKA polyclonal antibody. The results are as follows: figure 1 as shown ( figure 1 Among them, C1-C42 represent sample numbers, T represents cancer tissue, and N represents paracancerous tissue). Depend on figure 1 It can be seen that the band color of the cancer tissue in the randomly selected 43 cases of liver cancer samples is darker than that of the corresponding para-cancerous tissue, which indicates that the expression level of CHKA protein in the cancer tissue is significantly higher than that of the corresponding para-cancerous tissue.

[0041] It should be noted th...

Embodiment 2

[0087] In this example, 120 liver cancer patients were randomly selected to surgically excise tissues, and tissue sections of tumors of liver cancer patients were prepared, and the relative expression of CHKA protein in liver cancer tissues was detected by the above-mentioned immunohistochemical method. And adopt the score calculation method of the relative expression of CHKA protein of example 1 to obtain the score value of each liver cancer patient, according to the score value (the score value is greater than or equal to 9 is the CHAK high expression group, and the score value is lower than 9 is the CHAK low expression group ) to divide the patients with liver cancer into two groups, draw the overall survival curve and the disease-free survival curve (such as Figure 4 and Figure 5 As shown, n in the figure represents the sample size of this group, and the sample sizes of the high expression group and the low expression group are both 60). Depend on Figure 4 and Figure ...

Embodiment 3

[0089] This embodiment provides a kit for evaluating the prognosis of liver cancer, which includes the first antibody for immunohistochemical detection of the relative expression of CHKA protein in liver cancer tissue, namely CHKA polyclonal antibody, CHKA polyclonal antibody Prepared by immunizing rabbits with CHKA recombinant protein.

[0090] The kit can be used in the prognosis assessment of liver cancer. The CHKA polyclonal antibody of the kit is used to detect the relative expression of CHKA protein in the liver cancer tissue by immunohistochemical staining.

[0091] Of course, in other embodiments, the kit may also include a goat anti-rabbit anti-IgG antibody with horseradish peroxidase expression for anti-CHKA polyclonal antibody.

[0092] In this embodiment, the kit also includes immunohistochemical reagents, and the immunohistochemical reagents include: toluene, ethanol, 3% H 2 o 2 Methanol solution, antigen retrieval solution, blocking solution, DAB chromogenic re...

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Abstract

The invention discloses application of CHKA proteins in preparing a kit for prognostic evaluation of liver cancer. The expression amount of the CHKA proteins in the tumor tissue is determined by means of an immunohistochemical technique, microscope photographs and computer image processing software. The method for determining the expression amount of the CHKA can be used for judging the prognosis effect of the postoperative liver cancer patient, and provides important guidance for the postoperative monitoring and the sequential treatment of liver cancer patients. Moreover, the invention further discloses a kit for the prognostic evaluation of liver cancer and the method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application, kit and method of CHKA protein in the preparation of liver cancer prognosis assessment kit. Background technique [0002] Although the understanding of liver cancer has been greatly developed in recent years, the mortality rate related to liver cancer has shown a rising trend worldwide in recent years, and the 5-year overall survival rate of patients with liver cancer is still very low compared with some other tumors. The vast majority of patients with liver cancer do not die from the primary tumor, but mainly from the recurrence and metastasis of the tumor. Therefore, in-depth study of the molecular mechanism leading to the development and metastasis of liver cancer is undoubtedly of positive significance for improving the current clinical treatment plan. [0003] Choline kinase is the key enzyme in the first step in the process of catalyzing choline into phosphory...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6893G01N33/57438G01N33/57484
Inventor 王红阳陈磊
Owner 王红阳
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