The invention provides an immunohistochemical hematoxylin-
toluidine blue double-redyeing method for
melanocyte. The method comprises the following steps: (1), carrying out conventional
xylene dewaxing on a slice and carrying out gradient
alcohol dehydration; (2), blocking and inactivating endogenous
peroxide enzyme; (3), repairing an
antigen; (4), carrying out serum sealing; (5), dropwise adding primary antibodies; (6) dropwise adding secondary antibodies; (7), carrying out DAB developing; (8), carrying out hematoxylin redyeing and
hydrochloric acid alcohol differentiation; (9), redyeing by a
toluidine blue working solution and carrying out glacial
acetic acid differentiation; (10), dehydrating to be transparent and sealing the slice. According to the invention, positive granules are still brownish yellow after redyeing, and original
melanin granules in the tissue become deep green, so that distinction is obvious, the
melanin granules are easily distinguished from brown granules, the developing of hematoxylin is not influenced,
cell nucleuses of background cells are clearly visible, the
color difference of immunohistochemical positive brownish yellow granules, deep green
melanin granules and blue
cell nucleus is obvious and intuitive, and distinguishing is convenient.
Toluidine blue
dyeing liquid is prepared simply and conveniently.