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Mouse anti-human CD14 monoclonal antibody hybridoma cell line, monoclonal antibody, immunohistochemical reagent kit and uses thereof

A hybridoma cell line and monoclonal antibody technology, which is applied in the direction of anti-animal/human immunoglobulin, antibody, extracellular fluid diseases, etc., can solve the problems of abnormal platelet recovery and achieve good sensitivity

Inactive Publication Date: 2008-06-18
UNION STEMCELL & GENE ENG
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Problems solved by technology

However, it is the HLA-I alloantibodies in the PTR and rarely the HPA that is responsible for the abnormal recovery of platelets after transfusion

Method used

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  • Mouse anti-human CD14 monoclonal antibody hybridoma cell line, monoclonal antibody, immunohistochemical reagent kit and uses thereof
  • Mouse anti-human CD14 monoclonal antibody hybridoma cell line, monoclonal antibody, immunohistochemical reagent kit and uses thereof
  • Mouse anti-human CD14 monoclonal antibody hybridoma cell line, monoclonal antibody, immunohistochemical reagent kit and uses thereof

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Embodiment Construction

[0032] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0033] A mouse anti-human CD41 monoclonal antibody hybridoma cell line, the preservation number of the cell line is CGMCC NO.2177.

[0034] The mouse anti-human CD41 monoclonal antibody secreted by the hybridoma cell line can specifically bind to the CD41 antigen molecule.

[0035] An isolated and purified antigen can specifically bind to the mouse anti-human CD41 monoclonal antibody produced by the hybridoma with the deposit number CGMCC NO.2177.

[0036] The antibody-binding epitope is located in the conformational epitope of human CD41 antigen and CD61 antigen.

[0037] A fluorescein-labeled monoclonal antibody, said antibody is fluorescein-labeled on the basis of the monoclonal antibody described in claim 2, which can inhibit the aggregation caused by ADP, collagen and HIP2.

[0038] A SAP method immunohistochemical kit capable of rapidly an...

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Abstract

The present invention discloses a mouse anti-human CD41 monoclonal antibody hybridoma cell system, a monoclonal antibody and an immunohistochemical kit and an application thereof. A traditional hybridoma preparation technology is used to obtain hybridoma cell strain which excretes anti-human CD41 molecular monoclonal antibody stably. The excreted antibody can identify the conformational epitopes of the combination of human CD41 antigen and CD61 antigen and can inhibit the aggregation effect caused by ADP, collagen, HIP2, etc. The monoclonal antibody is marked biotin directly and combined with alkali phosphatase-strep avidin working fluid, substrate solution and fast red to form the immunohistochemical kit which uses SAP method to detect megakaryocyte. The kit and the detection method provided by the present invention can shorten the detection time and avoid repeated washing, so the status of cell chipping can not occur, the detection efficiency is improved greatly and the present invention is fit for common technicians to operate.

Description

technical field [0001] The invention relates to an anti-CD41 mouse-anti-human hybridoma cell line, a monoclonal antibody, and a SAP method immunohistochemical kit with the antibody labeled with biotin as the main active ingredient and its application. Background technique [0002] In malignant hematological diseases such as myelodysplastic syndrome (MDS), acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML), and myeloproliferative disorders (MPD), the megakaryotic system of the bone marrow will undergo qualitative and quantitative abnormalities , so that abnormal megakaryocytes and platelets appear in the bone marrow (sometimes even in peripheral blood). Most of these abnormal megakaryocytes have small nuclei and small cell bodies, so they are called "small megakaryocytes (SMK)". Small megakaryocytes can also be seen in patients with megaloblastic anemia. The appearance of small megakaryocytes in bone marrow and peripheral blood has important clinical signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/18C07K16/18G01N33/53G01N33/577A61K39/395A61P7/00A61P7/02
Inventor 郭桂庆何大水黄丽华廖晓龙屈浩佘鸣王冬梅袁向飞张金香张丽艳张毅张宇光周立
Owner UNION STEMCELL & GENE ENG
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