4 results about "Dot blot" patented technology

The application belongs to the technical field of biology and particularly relates to a gene typing dot array chip for detecting African swine fever virus and a construction method and application thereof. The application firstly provides a single nucleotide polymorphism site combination for precisely detecting African swine fever virus of genotype I, genotype II and I / II recombinant virus. Secondly, the application provides a gene typing dot array chip for detecting African swine fever virus, which can simultaneously realize precise typing, epidemic and variation monitoring and identification of the recombinant gene of I / II recombinant virus of high-throughput detection samples.

The invention relates to the technical field of antibody preparation, in particular to a CISD1 protein K68 site lysine lactylation modified antibody as well as a preparation method and application thereof. The antibody is obtained by immunizing an animal, and an immunogen comprises a polypeptide of which the sequence is C DMEDLGD-(Lactyl) K-AVY or C DMEDLGD-(Lactyl) K-AV. The invention provides a CISD1 protein K68 site lysine lactic acid modified antibody as well as a preparation method and application thereof. The CISD1 protein K68 site lysine lactic acid modified antibody is obtained by well-designed lactic acid modified polypeptide immune animals and multiple rounds of affinity purification; eLISA and Dot blot experiment results show that the antibody can efficiently distinguish CISD1 polypeptides in a lactic acid modification form from CISD1 polypeptides in a non-modification form, Western Blot and immunofluorescence experiment results show that the antibody can specifically recognize the lactic acid modification level and expression change of lysine at the CISD1 protein K68 site in human and mouse brain tissue samples; according to the invention, the purpose of successfully preparing the lysine lactylation modified antibody at the K68 site of the CISD1 protein for the first time is achieved, and the antibody can be applied to detection of in-vitro cells and in-vivo tissues of human and mice.

The application belongs to the technical field of biology and relates to a microfluidic sample pretreatment chip for detecting different genotype viruses of African swine fever and a construction method and application thereof. The application firstly provides a target gene SNP site combination for detecting African swine fever genotype I, II and I / II recombinant viruses; secondly, provides a LAMP system for specifically amplifying the target gene SNP site combination for detecting African swine fever genotype I, II and I / II recombinant viruses, and constructs a microfluidic sample pretreatment chip; finally, the sample for identifying the genotype of African swine fever virus is added into the microfluidic sample pretreatment chip for amplification, and the amplification product can be subjected to ASFV genotyping by detecting the above-mentioned SNP site combination on a genotyping dot blot chip, so that the detection, identification and monitoring of African swine fever genotype I, II and I / II recombinant viruses can be finally realized.

This invention relates to the field of clinical virus detection, and more particularly to a protein dot blot detection kit, detection method, and application for varicella-zoster virus (VZV). The kit includes: a solid-phase carrier membrane for binding and immobilizing proteins in the sample; a blocking agent for blocking non-specific binding sites on the solid-phase carrier membrane; a first antibody that specifically recognizes the VZV antigen; an enzyme-labeled second antibody that binds to the first antibody; and a chromogenic solution for generating a detection signal. This kit is based on protein dot blot technology, detecting proteins with high specificity and a low false-positive rate. The experimental cycle is short, taking only 2-3 hours from sample pretreatment to signal detection, much faster than virus culture (requiring 4-7 days) and PCR detection (although fast, prone to false positives or false negatives), providing rapid evidence for clinical treatment, and is particularly suitable for the diagnosis of acute herpes zoster.