Protein spot blot detection kit for varicella-zoster virus and application of protein spot blot detection kit

The varicella-zoster virus protein dot blot assay kit utilizes antigen-antibody reaction to detect VZV antigen, solving the problems of high sample requirements, high false positive rate, and strong equipment dependence of existing PCR technologies, and achieving rapid and low-cost detection results.

CN122084895APending Publication Date: 2026-05-26SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
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Patent Information

Application Number
CN202610287736.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-10
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

Current real-time quantitative PCR technology has several drawbacks in detecting varicella-zoster virus, including stringent sample requirements, susceptibility to interference from inhibitors, high false negative/false positive rates, strong equipment dependence, and high costs.

Method used

This invention provides a protein dot blot detection kit for varicella-zoster virus, which directly detects VZV antigen in samples through antigen-antibody reaction. It uses a solid-phase carrier membrane, blocking agent, specific antibody and chromogenic solution, which simplifies sample processing and reduces equipment dependence.

Benefits of technology

It enables rapid, low-cost, and low-false-positive-rate testing, making it suitable for primary healthcare institutions. It overcomes the inefficiency of virus culture and improves diagnostic efficiency.

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Abstract

The invention relates to the field of clinical virus detection, in particular to a chicken pox-herpes zoster virus protein spot blot detection kit, a detection method and application of the chicken pox-herpes zoster virus protein spot blot detection kit. The kit comprises a solid-phase carrier membrane used for combining and fixing protein in a to-be-detected sample; the sealing agent is used for sealing non-specific binding sites on the solid-phase carrier membrane; the first antibody is used for specifically recognizing a VZV antigen; the enzyme-labeled second antibody is combined with the first antibody; and the developing solution is used for generating a detection signal. The kit is based on a protein dot blot technology, the detected object is protein, the specificity is high, and the false positive rate is low. The kit has the advantages that the kit is simple in operation and short in experimental period, the whole process from sample pretreatment to signal detection is only 2-3 hours and is far faster than virus culture (4-7 days) and PCR (polymerase chain reaction) detection (although the detection is fast, false positive or false negative is easily caused), a basis can be quickly provided for clinical treatment, and the kit is particularly suitable for acute-stage diagnosis of herpes zoster.
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