Application of Shp2 protein in preparation of liver cancer prognosis evaluation kit

A liver cancer prognosis and kit technology, applied in the biological field, can solve the problems of unclear molecular mechanism and lack of specific molecular targets for treatment

Active Publication Date: 2013-04-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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Problems solved by technology

[0010] Liver cancer is one of the most threatening tumors to human health. The molecular mechanism of its occurrence is still unclear, and its treatment lacks specific molecular targets. Shp2, as a very important tumor oncogene, has no literature yet It is reported that Shp2 protein is related to the prognosis of liver cancer or the metastasis of liver cancer

Method used

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  • Application of Shp2 protein in preparation of liver cancer prognosis evaluation kit
  • Application of Shp2 protein in preparation of liver cancer prognosis evaluation kit
  • Application of Shp2 protein in preparation of liver cancer prognosis evaluation kit

Examples

Experimental program
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Effect test

Embodiment 1

[0055] The present invention adopts the immunohistochemical method to detect the relative expression of Shp2 protein in the liver cancer tissue, and the specific steps are:

[0056] (1) Paraffin sections of liver cancer tissues were prepared, and the oven was set at 60° C. overnight;

[0057] (2) slices are dewaxed to water;

[0058] (Xylene 10min→Xylene 10min→Xylene 10min→100% ethanol 5min→95% ethanol 5min→85% ethanol 5min→75% ethanol 5min→double distilled water 5min)

[0059] (3) 3%H 2 o 2 Methanol solution, place at room temperature for 20 minutes;

[0060] (4) Wash with double distilled water three times, each time for 5 minutes;

[0061] (5) Antigen retrieval: Put slices into 0.01M citrate buffer (pH6.0) and boil for 5 minutes, stop fire for 10 minutes, and boil for 5 minutes;

[0062] (6) Naturally cool to room temperature, wash with double distilled water three times, 5 minutes each time;

[0063] (7) Block with 1% BSA for 30 minutes at 37°C;

[0064] (8) Shake o...

Embodiment 2

[0079] Sample: a tissue section of a liver cancer patient's tumor, and the relative expression of Shp2 protein in the liver cancer tissue was detected by the above-mentioned immunohistochemical method test steps. Micrographs of liver cancer tissue such as image 3 shown. The calculated Shp2 immunohistochemical score of the cancer tissue was 0.46.

[0080] According to postoperative follow-up, the patient died 6 months after the operation, and the disease-free survival period was only 3 months.

Embodiment 3

[0082]Sample: a tissue section of a liver cancer patient's tumor, and the relative expression of Shp2 protein in the liver cancer tissue was detected by the above-mentioned immunohistochemical method test steps. Micrographs of liver cancer tissue such as Figure 4 shown. After calculation, the Shp2 immunohistochemical score of cancer tissue was 0.21.

[0083] According to the postoperative follow-up, the patient is alive and well without recurrence.

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Abstract

The invention belongs to the biotech field, relates to an application of an Shp2 protein, and concretely relates to an application of the Shp2 protein in preparation of a liver cancer prognosis evaluation kit. The relative Shp2 protein expression level of a tumor tissue to a tissue adjacent to the tumor is determined through utilizing an immunohistochemical technology, microscope shooting and computer image processing software, and postoperative follow-up information is combined to determine that the relative Shp2 protein expression level is related with the hepatocellular carcinoma patient prognosis after the operation, so the Shp2 protein can be used for preparing a protein molecule maker for determining the liver cancer patient, and is of great guiding significance to the postoperative monitoring and sequential treatment of hepatocellular carcinoma patients.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of Shp2 protein, in particular to the application of Shp2 protein in the preparation of liver cancer prognosis assessment kit. Background technique [0002] Liver cancer is a disease that seriously endangers human beings. my country is a country with a high incidence of liver cancer. The morbidity and mortality are on the rise, and the age of onset is younger. The annual expenditure on liver cancer treatment has greatly increased. Liver cancer has seriously endangered the lives and property of our people and affected social and economic development. Statistics show that the high metastasis recurrence rate after liver cancer surgery (5-year recurrence rate reaches 70%, small liver cancer also reaches 50%) has become a bottleneck for further improving long-term efficacy, and is also an important key to conquer liver cancer. The unknown mechanism of recurrence and metastas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 王红阳丁劲韩涛李恒宇向代民孙焕林
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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