Method for producing artificial skin melanoma tissue

Abstract

The invention provides a preparation method of artificial cutaneous melanoma tissues, including the culture of keratinocytes and melanoma cells of human skin as well as the preparation of biological matrix material. The keratinocytes and the melanoma cells of human skin are respectively inoculated on the biological matrix material in sequence, and by the adoption of a method combining culture under liquid and air-liquid level incubation, a certain culture medium is used under the conditions of 5 percent of CO2 and asepsis at 37 DEG C to culture and construct the cutaneous melanoma tissues. Methods of histology and immunohistochemistry technology are adopted for the observation of growth situation of the malignant cutaneous melanoma tissues. The melanoma tissues closer to the microenvironment of human skin are constructed by the preparation method and the observation by the histology method shows the obvious invasive growth of the melanoma tissues and the formation of obvious tumor metastases or tumor groups on the surface of HDED and in the lumen of the dermal accessory organ, thus meeting the distribution characteristics of the malignant melanoma tissues infiltrated in the dermis of human skin and reflecting that markers of the melanoma tissues show a positive reaction.

Description

technical field [0001] The invention relates to a method for making artificial skin melanoma tissue, in particular to a method for making skin melanoma tissue by using human skin keratinocytes, melanoma cells and matrix materials. Background technique [0002] Malignant melanoma (MM) is a highly malignant tumor arising from the melanocyte system of the skin and other organs, and is prone to metastasis. Generally, the prognosis of melanoma patients is poor, and the average survival time of advanced patients is Only 6 to 9 months old, it is a disease that seriously threatens people's health and life. In recent years, the incidence of this disease has been increasing year by year worldwide. It is generally believed that it may be due to the increase in exposure to ultraviolet rays, or it may be the result of a combination of various factors. At present, the pathogenesis and mechanism of malignant melanoma are still unclear, which brings difficulties in diagnosis and treatment....

AI Technical Summary

Problems solved by technology

[0005] 2. Although EvesP uses human decellularized dermal tissue (DED) as the matrix material, there are still some technical issues that are debatable. For example, (1) DED is relatively complicated to make, and the dermis is treated with 1mol / L sodium chloride for 6-8 hours. There is no test description for the basement membrane components on the surface; (2) The melanoma seed cells are exclusive to Ghanem's laboratory, and the source is limited; (3) During the planting process of the melanoma seed cells, in order to prevent the seed cells from detaching, they need to be fixed with a specific metal ring , which brings inconvenience to the actual operation, and cannot ensure that the seed cells adhere to the DED

However, this model has a single seed cell (only melanoma cells, lack of epidermal seed cells), and lacks certain epidermal histological support when estimating the tumor formation mechanism related to the interaction process between melanoma and epidermis