Tissue chip for detecting backbone metastatic carcinoma prognosis related molecule sign
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A technology of molecular markers and tissue chips, applied in the biological field, to improve the effect of clinical treatment
Inactive Publication Date: 2007-08-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Abstract
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Problems solved by technology
At present, normal tissue microarrays and tumor tissue microarrays have been published, but none of them are aimed at screening the prognosis-related molecular markers of spinal metastases.
Method used
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Examples
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Embodiment 1
[0019] Example 1: Spinal metastatic adenocarcinoma tissue chip and its fabrication
[0020] The gender, age, surgical resection method, visceral metastasis, bone metastasis, and tumor type of patients with clinical spine metastatic adenocarcinoma were collected, and the postoperative radiotherapy, chemotherapy, and survival status of patients were followed up (the deceased recorded the date of death, and the survivors recorded the final date of death). The last follow-up time and marked as censored data). Establish clinical data and follow-up database.
[0021] Spinal metastatic tumor specimens were collected, divided into approximately 5mm×15mm×15mm in size, and the tissue samples were routinely dehydrated and embedded in paraffin. 100 spinal metastatic adenocarcinoma specimens with complete clinical data and follow-up were selected as donor wax blocks.
[0022] The donor paraffin block was cut into 4 micron thick pathological tissue slices with a fully automatic paraffin s...
Embodiment 2
[0035] Embodiment 2: Use this chip to do the immunohistochemical analysis of MMP-9 gene
[0036] (1) Tissue sections were dewaxed and hydrated.
[0037] Before dewaxing, the tissue chips should be placed at room temperature for 60 minutes or baked in a 60°C incubator for 20 minutes.
[0038] (A) The tissue chip was soaked in xylene for 10 minutes, and then soaked for another 10 minutes after replacing the xylene;
[0039] (B) Soak in absolute ethanol for 5 minutes;
[0043]Add 0.01M sodium citrate buffer solution (pH6.0) in boiling water. Put the lid on the stainless steel pressure cooker, but do not lock it. Place the slide on the metal staining rack, apply pressure slowly to soak the slide in the buffer for 5 minutes, then lock the lid and the small valve will rise. After 10 minutes, remove the h...
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Abstract
The invention relates to the biotechnology area, and the spinal metastatic cancer prognosis judgment is the key to correct determine clinical development strategy, and in the invention, for the present spinal metastatic cancer related molecular biology prognosis judgment evaluation lacking problem, it constructs the spinal metastatic cancer prognosis related molecular marker tissue chip. By screening the clinical data and determining the random data spinal metastatic cancer pathological wax block to produce the spinal metastatic cancer tissue chip; selecting a variety of molecular markers and tissue chips for immunohistochemistry or in-situ hybridization, and through image analysis and quantitative measurement to analyze the expression instance of each molecular marker in the spinal metastatic cancer pathological specimens, and to analyze the relationship between the molecular markers expression and the patients prognosis, and screening the molecular markers which are closely related to the spinal metastatic cancer prognosis. The tissue chip provides a high-throughput, large sample, rapid detection tool for screening the spinal metastatic cancer prognostic related molecular markers, and provides a theoretical basis for the rational determination of the spinal metastatic cancer development strategies.
Description
Technical field: [0001] The invention relates to the field of biotechnology, in particular to a tissue chip for detecting molecular markers related to prognosis of spinal metastases. Background technique: [0002] Spinal metastatic cancer is an important disease that seriously endangers human health, with high disability and mortality rates. Metastatic cancer destroys the bone of the spine, causing vertebral segment collapse and spinal nerve compression, resulting in spinal nerve dysfunction, paralysis, and intractable pain, seriously affecting the quality of life and survival of patients. With the update of surgical treatment concepts and techniques, surgical treatment has increasingly become an important means of treatment for spinal metastatic tumors. However, due to the limited survival of patients with spinal metastases, surgical treatment is still an area of controversy. Facts have proved that inappropriate surgical treatment not only does not help patients to prol...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25C12Q1/68
Inventor 陈华江杨兴海
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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