4 results about "Cancer development" patented technology

Provided herein is a method for detecting a tumour that can be applied to cell-free samples, to cell-free detect circulating tumour DNA. The method utilizes detection of adjacent methylation signals within a single sequencing read as the basic positive tumour signal, thereby decreasing false positives. The method comprises extracting DNA from a cell-free sample obtained from a subject, bisulphite converting the DNA, amplifying regions methylated in cancer (CpG islands, CpG shores, and / or CpG shelves), generating sequencing reads, and detecting tumour signals. To increase sensitivity, biased primers designed based on bisulphite converted methylated sequences can be used. Target methylated regions can be selected from a pre-validated set according to the specific aim of the test. Absolute number, proportion, and / or distribution of tumour signals may be used for tumour detection or classification. The method is also useful in, predicting, prognosticating, and / or monitoring response to treatment, tumour load, relapse, cancer development, or risk.

Provided herein is a method for detecting a tumour that can be applied to cell-free samples, to cell-free detect circulating tumour DNA. The method utilizes detection of adjacent methylation signals within a single sequencing read as the basic positive tumour signal, thereby decreasing false positives. The method comprises extracting DNA from a cell-free sample obtained from a subject, bisulphite converting the DNA, amplifying regions methylated in cancer (CpG islands, CpG shores, and / or CpG shelves), generating sequencing reads, and detecting tumour signals. To increase sensitivity, biased primers designed based on bisulphite converted methylated sequences can be used. Target methylated regions can be selected from a pre-validated set according to the specific aim of the test. Absolute number, proportion, and / or distribution of tumour signals may be used for tumour detection or classification. The method is also useful in, predicting, prognosticating, and / or monitoring response to treatment, tumour load, relapse, cancer development, or risk.

A method for identifying the presence of HPV in human tissue may comprise the following steps: collecting a cell from oropharyngeal tissue of a subject utilizing a swab and measuring HPV DNA in the cell or in a solution that contains the contents of the cell. The method may include calculating a risk assessment for the development of cancer in the subject, wherein the risk assessment is calculated from data generated by the measuring of the HPV DNA to determine a persistence of the HPV in human tissue.