43 results about "Intestinal bacterium" patented technology

A method of preparing 1, 3- propylene glycol by high cell density intestinal uses the method of fermentation. Its process: seed culturing the intestinal bacterium is activated and then inoculated in the nutrient solution with no bacterium seed; the raises process of fermentation nutrient solution: when this solution is disinfected and cool, we inoculate the seed solution with the volume rate 3-15% into a tank of fermentation solution; the fast grow period of separated fermentation solution: the density of carbon retains 0.1-30g / L; high cell density fermentation period: the density of the bacterium seed reaches OD6502.0-5.0; it adapts the way of limited accommodate of alimentation and air; when the product has been synthesized, we add nitrogen, phosphate, inorganic ion, trace element,VB12 or their mixture of one or several components, and simultaneously add flowing glycerin; when there is acid, the fermentation process finishes.

The invention discloses a microbial fermentation feed for litopenaeus vannamei. The microbial fermentation feed is prepared by the following specific steps: after fully mixing photosynthetic bacteria and bacillus, adding brown sugar and uniformly mixing to obtain a mixture A; uniformly spraying the mixture A on a commercially available feed for litopenaeus vannamei to obtain a mixture B; filling the mixture B in a sealed container, and sealing at a normal temperature for fermenting, wherein the fermentation is completed when the temperature of the center of the sealed container rises to 45 DEG C; and taking out a fermented mixture B, air-drying and bagging to obtain the microbial fermentation feed for the litopenaeus vannamei. The microbial fermentation feed for the litopenaeus vannamei, provided by the invention, is suitable for a later charging amount rapid-increasing stage during aquaculture of the litopenaeus vannamei, and is a health care functional feed product which has the functions of increasing the feed utilization rate and the digestibility, protecting the liver of the litopenaeus vannamei and promoting the intestinal bacterium equilibrium.

The present invention provides a mulberry pomace chicken feed and a preparation method thereof. The mulberry pomace chicken feed comprises the following raw materials in weight percentage: 20-30% of mulberry pomace powder, 10-15% of corn flour, 10-12% of rice bran, 4-8% of molasses, 10-15% of soybean meal powder, 10-15% of wheat bran, 5-8% of mulberry leaf powder, 8-12% of mulberry branches, 1-5% of atractylodes lancea, 2-4% of mirabilite, 5-8% of dried orange peels, 2-3% of ammonium sulfate, 0.2-0.5% of monopotassium phosphate and 0.05-0.08% of magnesium sulfate. The provided chicken feed is rich in raw material variety and adequate in nutrition, and is free of any chemical medicines. The feed after fermentation is fragrant in smell, is high in protein content, and is good in palatability. The food intake of chicken flocks is increased, the growth and development of chicken is fast, and the daily weight gain of chicken is increased significantly. The chicken feed can improve the production performance of chicken, significantly improve the intestinal environment of chicken, promote intestinal bacterium growth and reproduction, and inhibit the growth of harmful bacteria.

The invention discloses a microbial fermentation feed for litopenaeus vannamei. The microbial fermentation feed is prepared by the following specific steps: after fully mixing photosynthetic bacteria and bacillus, adding brown sugar and uniformly mixing to obtain a mixture A; uniformly spraying the mixture A on a commercially available feed for litopenaeus vannamei to obtain a mixture B; filling the mixture B in a sealed container, and sealing at a normal temperature for fermenting, wherein the fermentation is completed when the temperature of the center of the sealed container rises to 45 DEG C; and taking out a fermented mixture B, air-drying and bagging to obtain the microbial fermentation feed for the litopenaeus vannamei. The microbial fermentation feed for the litopenaeus vannamei, provided by the invention, is suitable for a later charging amount rapid-increasing stage during aquaculture of the litopenaeus vannamei, and is a health care functional feed product which has the functions of increasing the feed utilization rate and the digestibility, protecting the liver of the litopenaeus vannamei and promoting the intestinal bacterium equilibrium.

The invention provides a primer compound for identifying intestinal microecological state and an application thereof. The primer compound is a specific primer designed for intestinal bacterium 16S rRNA; the specific primer includes random basic groups; the quantity of random basic groups is 3-5; the intestinal bacterium includes any one of or combination of at least two of symbiotic clostridia, bifidobacterium adolescentis, fusobacterium nucleatum, peptostreptococcus anaerobius or klebsiella. A kit researched and developed on the basis of the primer compound provided by the invention has a specificity for detecting intestinal microecological imbalance reaching up to 92.5% and a sensitivity reaching up to 80.43%.

The invention provides a compound which is stable in an acid, has an antibacterial effect against a bacterium Helicobacter pylori, can exert a satisfactory level of an antibacterial effect when used singly, does not affect an enteric bacterium, has an antibacterial effect against a bacterium resistant to an antibacterial agent, and has an inhibitory effect on gastric acid secretion; and a pharmaceutical composition comprising the compound. Specifically disclosed are: a compound represented by the general formula (I) or a salt thereof; and a pharmaceutical composition comprising the compound or the salt thereof. (I) wherein R represents a linear alkyl group having 4 to 8 carbon atoms, preferably 5 to 7 carbon atoms.

The invention relates to the application of mushroom dietary fiber extracts in the field of pharmaceutics, in particular to the application of the mushroom dietary fiber extracts for preparing a pharmaceutical preparation for treating and / or preventing diseases associated with intestinal bacterium community disorder and a preparation method thereof. The extracts are significantly beneficial to restoration of a dominant bacteria structure in intestines, diversity of bacteria is facilitated, significant treating and / or preventing effects on the diseases associated with an intestinal bacterium community structure are shown, for example, expression of inflammatory factors can be effectively inhibited to effectively relieve the deterioration of inflammation, each item of physiological and pathological indexes related to immune is improved, the extracts can be applied to the fields of medicine, health products and the like, and the extracts have significant economic and social value.

The invention discloses laminarinase OUC-L1. The amino acid sequence of the laminarinase OUC-L1 is shown as SEQ ID NO:1, the gene of the laminarinase OUC-L1 is encoded, and the nucleotide sequence ofthe laminarinase OUC-L1 is shown as SEQ ID NO. 2. The laminarinase OUC-L1 is explored from a human intestinal bacterium A. muciniphila genome, belongs to a glycoside hydrolase GH16 family, and can effectively hydrolyze laminarin, and the main product of the laminarinase OUC-L1 is three parts of oligosaccharides, has high specific enzyme activity and good biological catalysis efficiency, and has the highest reaction activity at the temperature of 35 DEG C. In addition, the laminarinase OUC-L1 also has good cold adaptability. The invention further discloses an enzyme preparation containing the laminarinase OUC-L1. The enzyme preparation still has considerable catalytic activity at low temperature and has good industrial application potential.

The invention aims to provide a traditional Chinese medicine for treating and / or preventing gastrointestinal dysfunction caused by critical diseases. The traditional Chinese medicine is prepared from the following raw materials: radix codonopsis, fried largehead atractylodes rhizome, poria cocos, honey-fried licorice root, Chinese yam, white hyacinth bean, coix seed, villous amomum fruit, balloonflower root, pomegranate rind, myrobalan, and red halloysite. The traditional Chinese medicine is prepared according to the theories of tonifying qi, invigorating the spleen, upbearing the clear, and downbearing the turbid, has an integral conditioning effect by simultaneously eliminating and supplementing; can promote the peristalsis and propulsion of gastrointestinal smooth muscle, improves the blood circulation of the intestinal walls, reduces the capillary permeability of the gastrointestinal tracts, relieves the tissue edema, promotes the discharge of dead tissues, inhibits the intestinal bacterium translocation and toxin absorption in the intestines, eliminates the free radicals, and antagonizes the system inflammation reaction. The traditional Chinese medicine for treating gastrointestinal dysfunction caused by critical diseases has a good effect, can control the incidence rate of gastrointestinal dysfunction below 19.05%, does not have any side or toxic effect during the clinical process, and is reliable and safe.

The present invention provides a method capable of detecting a cancer and / or a carcinogenic risk, and a reagent for the detection thereof. The present invention also provides a method for screening for a pharmaceutical drug for reducing a carcinogenic risk and a pharmaceutical composition. Since cancer development closely correlates with alteration in gut microbiota, cancer development and / or a risk of cancer development are detected by detecting alteration in gut microbiota and a secondary bile acid produced by an intestinal bacterium. The present invention further provides a method for screening for a pharmaceutical drug with a gut microbiota as an index, and a pharmaceutical composition.

The invention discloses application of bacteria in preparation of a synergist of an immune checkpoint inhibitor. According to the present invention, the human endogenous intestinal bacterium single bacterium oral preparation is combined with the immune checkpoint inhibitor, and the anti-tumor immune protection reaction is generated through the endogenous intestinal bacterium stimulation, such that the drug effect of the immune checkpoint inhibitor on a plurality of tumor species is significantly enhanced, the safety is good, the overall survival time of cancer patients is prolonged, and the treatment effect is good. The response rate of cancer immunotherapy crowds is improved, and cancer immunotherapy benefiting tumor patient crowds are expanded.

The invention discloses an intestinal bacterial composition for improving a cognitive function and relieving neuroinflammation and an application of the intestinal bacterial composition, and belongs to the technical field of biomedicine and microbiology, the intestinal bacterial composition is composed of a Lachnospiraceae bacterium, a Bacteroides multiforme and a Roseburiatestinae bacterium, and the intestinal bacterial composition is prepared from the following raw materials of the following raw materials of the intestinal bacterial composition: the Lachnospiraceae bacterium, the Bacteroides multiforme and the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium, the Roseburiatestinae bacterium and the like. Experimental research results show that a modular synergistic effect exists among the helicobacter hirsuticus, the bacteroides multiforme and the Roche enterobacter, and the pharmaceutical composition has a remarkable neuroprotective effect on a cognitive impairment mouse model and an Alzheimer disease mouse model, can improve the cognitive function and relieve neuroinflammation, and can be used for preparing a neuroprotective agent for treating the cognitive impairment mouse model and the Alzheimer disease mouse model. A new thought and method can be provided for treatment of cognitive impairment related diseases and neuroinflammation related diseases.

The invention discloses aspergillus terreus QT122 which is Aspergillus terreus QT122, and its preservation number is CCTCC M 2015240. The invention also discloses preparation methods of an aspergillus terreus QT122 fermentation broth, an aspergillus terreus QT122 fermentation broth crude extract and an aspergillus terreus QT122 metabolite. The invention also discloses an application of aspergillus terreus QT122. The aspergillus terreus QT122 fermentation broth, the aspergillus terreus QT122 fermentation broth crude extract and the aspergillus terreus QT122 metabolite all can be used for inhibiting radicle growth of amaranthus retroflexus or barnyard grass. The aspergillus terreus QT122 fermentation broth, the aspergillus terreus QT122 fermentation broth crude extract and the aspergillus terreus QT122 metabolite can be used in the preparation of a biopesticide with an inhibitory activity to radicle growth of amaranthus retroflexus and barnyard grass.

The invention relates to the technical field of comprehensive treatment of agricultural pests, and particularly provides a bactrocera cucurbitae double-trapping type attractant based on optimal matching of host and intestinal bacteria volatile matters and application of the bactrocera cucurbitae double-trapping type attractant. The attractant comprises an active component and a synergistic component, the active component is derived from balsam pear and towel gourd volatile matters and bactrocera cucurbitae intestinal bacterial metabolites, and comprises 2-methyl ethyl valerate, propyl hexanoate, ethyl hexadienoate, heptanal, benzyl alcohol, 3-methyl acetic acid-1-butanol, indole and 1-undecanol; and the synergistic component is myzobenzoic ketone. Active components are quickly released in the early stage of application, so that the attraction efficiency is remarkably improved; the synergistic component prolongs the trapping lasting period through the slow release effect, and the long-term trapping effect is ensured. All the components of the attractant provided by the invention are biological sources or environment-friendly chemical substances, the attractant meets the requirement of green prevention and control, can efficiently attract male and female adults at the same time, makes up the limitation that a traditional sex attractant only induces male adults or a food attractant only induces female adults, and is beneficial to more comprehensive control of population quantity.

The invention belongs to the field of biology, and specifically discloses Bacillus Velez D2406 isolated from the intestinal tract of soldier flies and its application. The preservation number of the Bacillus Velez is CCTCC NO: M2020874. fly growth, and can stably inhibit Staphylococcus aureus in vitro and in soldier fly larvae. The intestinal bacterium of the invention can increase the conversion rate of larvae, increase the reduction rate of materials, increase the weight gain rate of larvae, and stably inhibit Staphylococcus aureus in the isolated condition and in soldier fly larvae. The volatile compound 2,5-dimethylpyrazine produced by the bacteria has a significant inhibitory effect on zoonotic pathogenic bacteria. Inoculation of the intestinal bacteria into the Soldier Fly transformation system not only improved the transformation efficiency of Soldier Fly larvae, but also reduced the abundance of Staphylococcus aureus in the transformation material, and it is expected to reduce the incidence of bacteria brought about by the use of chemical drugs. environmental pollution.

The invention discloses an intestinal bacterium-derived melatonin synthetase and an application thereof. Specifically, the melatonin synthetase is selected from one or more of a DDC enzyme, an AANAT enzyme and an AST enzyme, the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%, and the DDC enzyme comprises an amino acid sequence as shown in SEQ ID NO.2 or an active fragment of the amino acid sequence as shown in SEQ ID NO.2 or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.2 is more than 80%. The AANAT enzyme comprises an amino acid sequence as shown in SEQ ID NO.34 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.34 is more than 80%, and the AST enzyme comprises an amino acid sequence as shown in SEQ ID NO.42 or an active fragment thereof, or an analogue of which the similarity with the amino acid sequence as shown in SEQ ID NO.42 is more than 80%. On one hand, the melatonin synthetase can be used for improving the melatonin level of a host and improving related diseases; on the other hand, the method can be used for biosynthesis of melatonin and has a good application prospect.

According to the embodiments, an information processing system (1, 2) comprises: an acquisition unit (101, 301) that acquires an image captured of the color of a color reagent of a color reaction that uses the feces of a subject; and a determination unit (103, 303) that determines intestinal environment information about the state of the intestinal environment of the subject on the basis of the captured image. The color reagent is a reagent that changes color depending on the pH or polarity of a biological sample in the feces. The determination unit (103, 303) determines the concentration or abundance of an intestinal metabolite and at least one of the abundance ratio, abundance, and Shannon index of an intestinal bacterium as the intestinal environment information.

Provided in the present invention is an engineered bacterium, the engineered bacterium comprising an exogenous cyuP gene and / or cyuA gene, the exogenous cyuP gene encoding a cysteine transporter protein, the exogenous cyuA gene encoding a cysteine desulfurase, and the engineered bacterium being an intestinal bacterium or a probiotic.

The invention relates to a disease-oriented nutrient intervention method and system, equipment and a storage medium, and the method comprises the steps: building a query database, wherein an incidence relation of the diseases, intestinal bacteria and nutrients is recorded in the query database; inputting a target disease into the query database, obtaining each pathogenic intestinal bacterium of the target disease and taking the pathogenic intestinal bacterium as a target bacterium, and obtaining a target nutrient commonly associated with each target bacterium; and determining the intervention effect of the target nutrient on the target disease according to the action effect of the target nutrient on each target bacterium and the action effect of each target bacterium on the target disease. According to the method and system, the intervention effect of the target nutrient on the target disease can be rapidly and effectively determined, different human body constitutions are overcome, and therefore a nutrient intervention scheme is provided for chronic diseases related to intestinal bacteria.

The present invention provides an oral composition for potentiating the production of equol by an intestinal bacterium which contains, as the active ingredient, difructose anhydride for activating the equol productivity of the intestinal bacterium.

The invention discloses an intestinal flora marker for predicting the curative effect of immunotherapy and application of the intestinal flora marker. The inventor screens out DNA detection targets with expression differences in patients with different clinical effects from 55 intestinal bacterium DNA detection targets, and can solve the problems that few biomarkers can be available for melanoma immunotherapy patients and the effect is not ideal. The relationship between the intestinal flora characteristics and the PD-1 / PD-L1 blocking treatment may have generality in different types of tumors; the research results of the project have the possibility of being applied to tumors except melanoma, such as sarcoma, kidney cancer, lung cancer, stomach cancer or colorectal cancer, and benefiting patients through data supplementation and reanalysis.

The present invention relates to a novel species of Enterobacter, Enterobacter sp. 638, and to increased plant growth, e.g. Use of methods of growing, increasing plant biomass, increasing plant fruit and / or seed productivity, increasing plant disease tolerance and / or resistance and increasing plant drought tolerance and / or drought resistance. The method includes applying to the plant an effective amount of a composition comprising an isolated culture of Enterobacter 638.

The invention discloses a judgment marker for cognitive impairment of Parkinson's disease and application thereof The inventor of the invention finds that the relative abundance of the intestinal bacteria in the excrement of a PD patient is significantly related to PD-MCI, and the concentrations of isobutyric acid and isovaleric acid are related to the overall cognitive level. The composition characteristics of intestinal flora and metabolites of a PD-CI patient are researched from the metagenome level and the metabolome level, and the pathological mechanism of the PD-CI can be more comprehensively understood; and early intervention is also facilitated to slow down or treat the development of PD-CI.

The invention discloses application of enterobacter murine in preparation of a medicine for preventing or treating depression, and belongs to the field of medicine. The invention relates to an application of enterobacter murine in preparation of a medicine for preventing or treating depression. According to the present invention, the enterobacter muricatum is Muriaculum gardrongarter, and is preserved in the German Microbiological Culture Collection Center, and the preservation number is DSM 108194. The enterobacter muricatum can be used for preparing the enterobacter muricatum, the invention discloses application of active enterobacter murine, namely application of the active enterobacter murine in preparation of drugs for preventing or treating depression. The sucrose preference of the mouse is improved to a certain extent, the mood disorder is obviously reduced after the enterobacter murine is administered, and compared with fluoxetine, depression caused by chronic social contusion stress can be more quickly improved. The medicine is suitable for clinical popularization and use.

The invention belongs to the technical field of biological medicines, and particularly relates to application of butyric acid-producing enteromonas in prevention and treatment of senile deafness. It is found for the first time that the butyric acid producing enteromonas sp. Has a remarkable hearing protection effect in a senile deafness model; the action mechanism is mainly embodied in that by improving hearing threshold rise caused by aging, the structural integrity of cochlea hair cells is partially protected to relieve arrangement disorder and deletion of outer hair cells, and the number of inner ear spiral neuron ganglions and vascular stripe aging cells is effectively reduced; meanwhile, the high expression of Pvalb key genes and Parvalbumin in neurons and hair cells of spiral ganglions is up-regulated, so that the synergistic effect is achieved by enhancing the sound transmission muscle efficiency of the middle ear, improving the blood vessel microcirculation of the inner ear, stabilizing the hair cytoskeleton and protecting nerves. The invention provides a new candidate strain resource and an intervention target for preventing and treating senile deafness, and has important clinical application prospect and social value.

With the aim of proving an antibacterial composition against oral bacteria and the like capable of inducing Th1 cell proliferation or activation in an intestinal tract, the present inventors have found out that bacteria that suppress colonization and the like of the oral bacteria and the like in the intestinal tract are present in an intestinal microbiota. Moreover, the present inventors have succeeded in isolating intestinal bacteria that suppress intestinal colonization and the like of oral bacteria and the like.

A method of preparing 1, 3- propylene glycol by high cell density intestinal uses the method of fermentation. Its process: seed culturing the intestinal bacterium is activated and then inoculated in the nutrient solution with no bacterium seed; the raises process of fermentation nutrient solution: when this solution is disinfected and cool, we inoculate the seed solution with the volume rate 3-15% into a tank of fermentation solution; the fast grow period of separated fermentation solution: the density of carbon retains 0.1-30g / L; high cell density fermentation period: the density of the bacterium seed reaches OD6502.0-5.0; it adapts the way of limited accommodate of alimentation and air; when the product has been synthesized, we add nitrogen, phosphate, inorganic ion, trace element,VB12 or their mixture of one or several components, and simultaneously add flowing glycerin; when there is acid, the fermentation process finishes.