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Bacillus Velez d2406 isolated from the intestinal tract of soldier flies and its application

A Bacillus, Velez technology, applied in the biological field, can solve the problems of the increase of antibiotic-resistant pathogens, the increase of the possibility, the threat to the environment and human health, etc., to reduce the abundance, increase the reduction rate, and improve the efficacy.

Active Publication Date: 2021-12-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This widespread use of antibiotics has led to the presence of a large number of antibiotic resistance genes (ARGs) in animal manure, and the possibility of ARGs being transmitted to human pathogens through horizontal gene transfer has increased, making antibiotic-resistant genes in livestock and poultry manure and animal carcasses The increase of sexual pathogenic bacteria poses a serious threat to the environment and human health

Method used

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  • Bacillus Velez d2406 isolated from the intestinal tract of soldier flies and its application
  • Bacillus Velez d2406 isolated from the intestinal tract of soldier flies and its application
  • Bacillus Velez d2406 isolated from the intestinal tract of soldier flies and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Obtaining of Bacillus velezensis D2406:

[0032] The Bacillus Velez of the present invention is isolated from the intestinal tract of the soldier fly larvae, with Gram-positive rod-shaped spores. The strain has been sent to the China Center for Type Culture Collection on December 8, 2020 for preservation and classification. Name: Bacillus velezensis D2406, deposit number: CCTCC NO: M2020874, address: Wuhan University, Wuhan, China.

[0033] The Bacillus velezensis D2406 has the morphological characteristics and culture mode of conventional Bacillus velezensis.

Embodiment 2

[0035] Antibacterial effect of Bacillus velezensis D2406:

[0036] Pick a single colony of indicator bacteria and inoculate it into LB liquid medium, culture at 37°C with shaking at 180r / min for 16 hours, take 200 μL of the fermentation broth of indicator bacteria and add it to 100mL of LB solid medium that is about to solidify, shake gently and pour it into the plate. Pick a single colony of Bacillus veleisi D2406 and inoculate it into LB liquid medium (with sterile LB liquid medium as blank control), and culture at 28°C with shaking at 180r / min for 36h; take the supernatant of the bacterial fermentation broth (fermentation broth After centrifugation at 8,000 people / min for 10 min, the supernatant was passed through a 0.22 μm filter head to obtain the fermentation broth supernatant) 200 μL was added to the Oxford cup on the indicator plate, and the diagonal lines were 2 parallel. Culture at 37°C statically. After 24h or 48h, observe whether there is an antibacterial zone aro...

Embodiment 3

[0041] Application of Bacillus velezensis D2406 in promoting the growth of the light-spotted soldier fly:

[0042]Inoculate 1% (v / w) fermented liquid of Bacillus Velez D2406 in fresh pig manure (with the same volume of inoculated LB liquid medium as a control), and inoculate soldier fly larvae raised to 6 days old on artificial diet into In the above-mentioned pig manure (1500 head / kg pig manure), allow it to eat freely, treat that 1% larva begins to pre-pupa, and the worm material separates, and the effective bacterial concentration of described Bacillus Velez D2406 fermented liquid is 10 9 cfu / mL.

[0043] The harvested larvae and material residues were used to detect and analyze the material reduction rate, soldier fly conversion rate and soldier fly weight gain rate, and the golden yellow color naturally present in pig manure was detected using Staphylococcus aureus selective medium (Baird-Parker agar) Staphylococcus abundance changes.

[0044] The result is as follows: ...

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Abstract

The invention belongs to the field of biology, and specifically discloses Bacillus Velez D2406 isolated from the intestinal tract of soldier flies and its application. The preservation number of the Bacillus Velez is CCTCC NO: M2020874. fly growth, and can stably inhibit Staphylococcus aureus in vitro and in soldier fly larvae. The intestinal bacterium of the invention can increase the conversion rate of larvae, increase the reduction rate of materials, increase the weight gain rate of larvae, and stably inhibit Staphylococcus aureus in the isolated condition and in soldier fly larvae. The volatile compound 2,5-dimethylpyrazine produced by the bacteria has a significant inhibitory effect on zoonotic pathogenic bacteria. Inoculation of the intestinal bacteria into the Soldier Fly transformation system not only improved the transformation efficiency of Soldier Fly larvae, but also reduced the abundance of Staphylococcus aureus in the transformation material, and it is expected to reduce the incidence of bacteria brought about by the use of chemical drugs. environmental pollution.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to Bacillus Velez D2406 isolated from the gut of a soldier fly and its application. technical background [0002] As a large agricultural country, my country produces as many as 3.8 billion tons of livestock and poultry manure every year. These wastes seriously pollute the environment, spread diseases, and threaten human safety. Pathogenic microorganisms include bacteria, fungi, viruses, parasitic protozoa, and worms. However, due to weak awareness of environmental protection, backward waste treatment technology and facilities, and the consideration of production and treatment costs by farms, the development of livestock and poultry farming has brought environmental pollution and threatened the health of humans and animals. The study found that the pathogenic bacteria in livestock and poultry manure mainly include actinomycetes, salmonella, campylobacter, clostridium, corynebacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N43/60A01P1/00A61K31/4965A61P31/04A23K10/12A23K10/18A23K50/00C12R1/07
CPCA01N63/22A01N43/60A61K31/4965A61P31/04A23K10/12A23K10/18A23K50/00
Inventor 张吉斌肖小朋张元谱邹进发张永昊王斌彬喻子牛
Owner HUAZHONG AGRI UNIV
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