Double-label immunohistochemical staining kit used for differential diagnosis of metastatic renal cell carcinoma

A technique for renal cell carcinoma and immunohistochemistry, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of insufficient tissue volume of puncture biopsy specimens, difficult to diagnose, time-consuming and laborious, etc., and achieve intuitive comparison of experimental results and staining steps. The effect of less and shorter experiment time

Inactive Publication Date: 2015-01-21
卢洪胜
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  • Application Information

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Problems solved by technology

[0005] In view of the deficiencies in the above existing technologies, in order to overcome the histological features of metastatic renal cell carcinoma, which are easily confused with adrenal tumors, lung cancer, liver

Method used

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  • Double-label immunohistochemical staining kit used for differential diagnosis of metastatic renal cell carcinoma
  • Double-label immunohistochemical staining kit used for differential diagnosis of metastatic renal cell carcinoma
  • Double-label immunohistochemical staining kit used for differential diagnosis of metastatic renal cell carcinoma

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The research object is formaldehyde-fixed-paraffin-embedded metastatic clear cell renal cell carcinoma tissue (Department of Pathology, Taizhou Central Hospital), and the chemical experiment steps are as follows:

[0034] 1. Dry-bake the tissue slices in a 70°C incubator for 2 hours.

[0035] 2. Conventional xylene dewaxing for 3 times, 10 minutes each time, hydration in 100%, 100%, 95%, 85%, 75% gradient ethanol, 5 minutes each, and finally rinsed with distilled water.

[0036] 3. Directly boil and repair in EDTA antigen retrieval solution with pH 9.0, cook for 8-10 minutes, keep warm for 10 minutes, cool down to room temperature naturally, rinse with distilled water, and rinse with PBS for 3×3 minutes.

[0037] 4. Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0038] 5. Block with normal animal serum for 10 minutes, shake off the excess serum, add dropwise the primary antibody mixtu...

Embodiment 2

[0044] The research objects were formaldehyde-fixed-paraffin-embedded metastatic renal clear cell carcinoma tissues (Department of Pathology, Taizhou Central Hospital),

[0045] 1. Dry-bake the tissue slices in a 70°C incubator for 2 hours.

[0046] 2. Conventional xylene dewaxing for 3 times, 10 minutes each time, hydration in 100%, 100%, 95%, 85%, 75% gradient ethanol, 5 minutes each, and finally rinsed with distilled water.

[0047] 3. Directly boil and repair in EDTA antigen retrieval solution with pH 9.0, cook for 8-10 minutes, keep warm for 10 minutes, cool down to room temperature naturally, rinse with distilled water, and rinse with PBS for 3×3 minutes.

[0048] 4. Incubate in 3% hydrogen peroxide at room temperature for 10 minutes to block endogenous peroxidase, wash with PBS for 3×3 minutes.

[0049] 5. Block with normal animal serum for 10 minutes, shake off excess serum, add PAX8 antibody working solution dropwise without washing.

[0050] 6. Add the HRP enzyme-l...

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Abstract

The invention relates to the technical field of immunohistochemical staining and particularly relates to a double-label immunohistochemical staining kit used for differential diagnosis of metastatic renal cell carcinoma. The kit comprises a DAB developing solution and a primary antibody formed by mixing a mixed solution of a PAX8 antibody and a CD10 antibody or/and an HRP enzyme-labeled secondary antibody. In order to overcome the disadvantages that the histological characteristics of the metastatic renal cell carcinoma are extremely easily confused with adrenal tumors, lung cancer, liver cancer, thyroid cancer and the like so that a definite diagnosis is not easy to make, a conventional immunohistochemical method is used for respectively detecting, more time and labor are consumed, the tissue amount of an aspiration biopsy specimen is not enough and the like, the same sensitivity and specificity when each antibody in the mixed antibody is singly stained are kept by using the method; and the double-label immunohistochemical staining kit also has the advantages that staining steps are few, the experiment time is short, the stability is high, an experiment result can be directly compared, and brought information amount is much higher than that of traditional single staining and the like, and especially has the remarkable advantages for biopsy pathological diagnosis with few specimen amounts.

Description

technical field [0001] The invention relates to the technical field of immunohistochemical staining, in particular to a double-labeled immunohistochemical staining kit for differential diagnosis of metastatic renal cell carcinoma. Background technique [0002] Renal cell carcinoma (RCC) accounts for 2% to 3% of malignant tumors in the whole body, and ranks second among malignant tumors of the male reproductive system in the urinary system, accounting for about 15%, second only to bladder cancer. Due to the advancement of auxiliary examination methods and people's increasing attention to health, the rate of incidental renal cancer in new renal cancer has risen to more than 60%, and nearly 50% of the new cases are patients in routine physical examination or due to other diseases. B found overtime. However, among new cases, about 20%-30% of patients have metastases at the time of diagnosis; another 25% of patients develop metastases several years after radical nephrectomy or n...

Claims

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57438
Inventor 卢洪胜章辉
Owner 卢洪胜
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