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Preparation method of brain tissue rapid freezing section

A technology for frozen section and brain tissue, applied in the field of biomedicine, can solve the problems of low melting point of liquid nitrogen and frozen tissue, and achieve the effect of ensuring quality, reducing the formation of ice crystals, and reducing animal perfusion

Active Publication Date: 2014-03-26
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Animal tissues that have not been perfused can be analyzed for molecular and biochemical indicators as needed. However, due to the low melting point of liquid nitrogen, the direct quick freezing method of liquid nitrogen is easy to freeze the tissue

Method used

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  • Preparation method of brain tissue rapid freezing section
  • Preparation method of brain tissue rapid freezing section
  • Preparation method of brain tissue rapid freezing section

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: the preparation method of mouse brain tissue section

[0041] method:

[0042] 1. Sample collection and freezing method

[0043] 1. Prepare a cylindrical lidless tin paper box with a bottom surface diameter of 1.5 cm and a height of 2.5 cm. The bottom surface is required to be flat, and it is used to hold the frozen embedding agent OCT, and mark it on the tin paper box;

[0044] 2. Prepare a cylindrical fast freezing device with a cover bottom surface of 3.0 cm in diameter and 3.0 cm in height, such as figure 1 As shown, the handle 1 is included, and the handle 1 is used for convenient hand holding. The handle 1 is connected to the outer bladder 5. The inner tank 3 is provided inside the outer bladder 5, and a liquid-containing area 6 is formed between the outer bladder 5 and the inner tank 3. A connecting ring 7 is arranged between the upper end of the outer bag 5 and the upper end of the inner bag 3 , the connecting ring 7 seals the liquid-filled area...

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Abstract

The invention discloses a preparation method of a brain tissue rapid freezing section. According to the method, acetone is used as a freezing embedding medium and a liquid nitrogen intermediate medium, so that brain tissue can be rapidly frozen , and formation of ice crystals on the brain tissue can be effectively reduced; besides, factors affecting manufacturing quality, such as temperatures during material drawing and sectioning, fixing and soring conditions after sectioning and the like, of the freezing section are optimized, and the quality of the finally prepared section is further guaranteed. According to the brain tissue rapid freezing section obtained with the method, the freezing section which has stability and can well protect antigens and the enzyme activity is provided for further immunofluorescence, immunohistochemistry and in situ hybridization tests on the brain tissue, and a pathological section meeting requirements is provided for future clinical diagnosis related to nervous system diseases and scientific researches. Particularly, molecular and biochemical index detection can be further performed on other tissue of a mouse which is not subjected to perfusion treatment in the scientific research.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing quick frozen sections of brain tissue, and also a method for quick pathological diagnosis of diseases. Background technique [0002] Frozen section is a method of making the tissue reach a certain hardness by means of low temperature conditions and then sectioning, because it does not need to go through long-term fixation, embedding and post-sectioning steps, and can well preserve the antigens and enzymes of the tissue Therefore, it is widely used in clinical rapid pathological diagnosis and scientific research on diseases. Since the quality of frozen sections directly affects the accuracy of pathological diagnosis, it is of great significance to prepare high-quality frozen sections. However, frozen sections are more demanding and difficult than general paraffin sections. In scientific research experiments, frozen sections, immunohistochemistry and immunofluores...

Claims

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Application Information

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IPC IPC(8): G01N1/42G01N1/30
Inventor 高旭彭亚会惠洋马宁周凌云
Owner HARBIN MEDICAL UNIVERSITY
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