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Enteral microorganism transplanting process

A technology of intestinal microorganisms and processes, which is applied in the fields of separation of microorganisms, drug combinations, medical preparations with inactive ingredients, etc., can solve the problems of long shelf life, not a good source of transplantation, intestinal damage, etc. period, maintain the number of viable bacteria, and reduce the effect of migration

Pending Publication Date: 2020-11-06
广西细微生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1) At present, the flora libraries at home and abroad are all prepared as liquids. Although the production is simple, a colonoscope must be used for transplantation. Under anesthesia, the colonoscope is inserted from the anus, from the descending colon to the transverse colon to the ascending colon , and finally reach the blind end, and then spread the bacterial solution over the entire cecum while withdrawing. The whole operation is first of all a risk of anesthesia, and accidents that may occur during anesthesia
Second, colonoscopy may cause physical damage to the bowel
[0008] 2) Bacterial liquid transplantation is very inconvenient to use and must be hospitalized for transplantation, which greatly increases medical costs
[0009] 3) At present, the intestinal flora donated by voluntary donors are used at home and abroad. According to reports, the flora compatibility between mothers and children, brothers and sisters in the family is the best, so voluntary donations are used blindly. The intestinal flora of the patient may not be a very good source of transplantation
[0010] Therefore, it is a technical problem to be solved to develop a kind of gut microbial transplantation product with long shelf life and high gut microbial count

Method used

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  • Enteral microorganism transplanting process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A technique for transplanting intestinal microbes, comprising the steps of:

[0038] 1) Stool sample collection:

[0039] ①Select fecal samples donated by qualified donors, pack them in disposable food boxes and plastic bags, send them to the laboratory within one hour, and weigh them;

[0040] ② Add physiological saline at a weight ratio of 1:3, homogenize at a medium speed for 5 minutes, filter at 0.2 μm, and remove the residue;

[0041] ③ Detect the filtrate to obtain a qualified filtrate without obvious precipitation and abnormal color;

[0042] 2) Extraction of intestinal microorganisms:

[0043] Put the filtrate into a centrifuge tube, centrifuge at 1000rpm for 20 minutes to remove the precipitate, put the supernatant into a new centrifuge tube, centrifuge at 3000rpm for 10 minutes, discard the supernatant, wash the precipitate with normal saline, and centrifuge again at 3000rpm for 10 minutes, repeat three times , the final obtained liquid is filtered with a 6...

Embodiment 2

[0054] A technique for transplanting intestinal microbes, comprising the steps of:

[0055] 1) Stool sample collection:

[0056] ①Select fecal samples donated by qualified donors, pack them in disposable food boxes and plastic bags, send them to the laboratory within one hour, and weigh them;

[0057] ② Add physiological saline at a weight ratio of 1:7, homogenate at a medium speed for 8 minutes, filter at 5.0 μm, and remove the residue;

[0058] ③ Detect the filtrate to obtain a qualified filtrate without obvious precipitation and abnormal color;

[0059] 2) Extraction of intestinal microorganisms:

[0060] Put the filtrate into a centrifuge tube, centrifuge at 2000rpm for 10 minutes to remove the precipitate, put the supernatant into a new centrifuge tube, centrifuge at 4000rpm for 5 minutes, discard the supernatant, wash the precipitate with normal saline, and centrifuge again at 4000rpm for 5 minutes, repeat three times , the final obtained liquid is filtered with a 40 ...

Embodiment 3

[0071] A technique for transplanting intestinal microbes, comprising the steps of:

[0072] 1) Stool sample collection:

[0073] ①Select fecal samples donated by qualified donors, pack them in disposable food boxes and plastic bags, send them to the laboratory within one hour, and weigh them;

[0074] ② Add physiological saline at a weight ratio of 1:5, homogenate at a medium speed for 10 minutes, filter at 3.0 μm, and remove the residue;

[0075] ③ Detect the filtrate to obtain a qualified filtrate without obvious precipitation and abnormal color;

[0076] 2) Extraction of intestinal microorganisms:

[0077]Put the qualified filtrate into a centrifuge tube, centrifuge at 1500rpm for 15 minutes to remove the precipitate, put the supernatant into a new centrifuge tube, centrifuge at 3500rpm for 8 minutes, discard the supernatant, wash the precipitate with normal saline, centrifuge again at 3500rpm for 8 minutes, repeat Three times, the finally obtained liquid is filtered wit...

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Abstract

The invention discloses an enteric microorganism transplanting process. The process comprises the following steps: 1) collecting of an excrement sample; 2) extracting of intestinal microorganisms; 3)preparation of an intestinal microbial preparation: intestinal microbial enema, intestinal microbial capsules and intestinal microbial freeze-dried capsules; and (4) transplanting of the intestinal microorganisms: transplanting the intestinal microorganism preparation obtained in the step (3) in an enema or oral administration manner. The process is used for treating intestinal diseases caused byintestinal microorganism disorder, and immune system and nervous system diseases caused by intestinal microorganism disorder.

Description

【Technical field】 [0001] The invention relates to the technical field of microbial medicine, in particular to a technology for transplanting intestinal microorganisms. 【Background technique】 [0002] At present, people's living environment is getting cleaner and cleaner, and food packaging is getting more and more exquisite, which ensures that it meets the hygienic requirements, that is, there is absolutely no bacteria exceeding the standard. However, modern research shows that there are fewer and fewer beneficial microorganisms in the existing environment, and there are fewer and fewer beneficial microorganisms in people's bodies. On the contrary, there are more and more "harmful" microorganisms in the environment and in the body. Modern scientific research shows that intestinal flora plays a vital role in human health. The intestinal microbes of the human body maintain a dynamic balance with the intestinal environment. In some cases, this balance is broken, and the intest...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K47/36A61K47/10A61K47/46A61K9/10A61K9/48A61P1/00A61P37/02A61P25/00C12N1/02
CPCA61K35/74A61K47/36A61K47/10A61K47/46A61K9/10A61K9/4858A61K9/4866A61K9/4875A61P1/00A61P37/02A61P25/00C12N1/02
Inventor 黄春松兰云来刘因强
Owner 广西细微生物科技有限责任公司
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