Method for preparing stationary liquid for slicing and application of stationary liquid for slicing

Abstract

The invention relates to a method for preparing stationary liquid for slicing and application of the stationary liquid for the slicing, and belongs to the technical field of clinical medicine application. The method comprises the steps that methyl alcohol, glutaraldehyde and diethyl ether are mixed, and PBS buffer solutions are used for attenuation; saccharose is added to the solutions to be fully mixed, a pH value is regulated, and the stationary liquid for the slicing is obtained. Tissues are treated through pretreatment, saccharose placing, embedding, slicing and washing. The method is simple in working progress and step, the operating work amount of technical personnel is reduced, the operating steps of the whole process are few, needed time is shortened, tissues free of dehydration and waxdip can be kept fresh more highly, antigens for immunohistochemistry are kept complete and excellent, more accurate diagnosis conditions can be provided, xylene and other highly-polluted poisonous reagents are not needed, environmental pollution is reduced, and the guarantee for the body health of the technical personnel is enhanced.

Description

technical field [0001] The invention relates to a configuration method of a fixative for slices and an application thereof, belonging to the technical field of clinical application. Background technique [0002] Histopathological examination has been used in medical history for more than 100 years. Through pathological tissue biopsy examination, many diseases, especially tumors and other diseases, can be finally diagnosed. However, with the development of medicine, in routine tumor pathological diagnosis, there are many cases where it is difficult to make a clear morphological diagnosis only by H.E. staining. [0003] In recent years, with the development of clinical tumor diagnosis and treatment techniques, as well as the development of immunohistochemical techniques and the emergence of various specific antibodies, many difficult tumors have been more clearly identified through special immunohistochemistry. diagnosis. Especially for certain difficult tumors, the correct ...

AI Technical Summary

Problems solved by technology

[0011] In the past, immunohistochemistry was usually performed on paraffin sections or direct frozen sections, but since paraffin sections were processed through dehydration and dewaxing, the antigenicity of the tissue was greatly lost during this series of treatments. Some effective antigenic properties even completely disappear. Although some antigen restoration techniques have been used, it is still difficult to reach the level before the treatment. This makes the immunohistochemical diagnosis of paraffin sections have huge defects, and the diagnostic results often cannot reflect the disease correctly. Even sometimes misleading

However, for conventional direct frozen sections, due to the rapid expansion of water in the tissues during freezing, the formed ice crystals burst through the biofilm structures such as cell membranes, making it difficult to maintain a good shape of the sections. This situation will also make the positioning of the diagnosis meaningless, and even appear possibility of false positive