Method for preparing stationary liquid for slicing and application of stationary liquid for slicing

A configuration method and fixative technology, applied in the field of clinical application, can solve the problems that the diagnosis result cannot correctly reflect the condition, the diagnosis positioning is meaningless, and the defects of immunohistochemical diagnosis, etc., so as to enhance physical health, enhance security, and correct diagnosis conditional effect

Inactive Publication Date: 2013-12-25
吴天骥
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] In the past, immunohistochemistry was usually performed on paraffin sections or direct frozen sections, but since paraffin sections were processed through dehydration and dewaxing, the antigenicity of the tissue was greatly lost during this series of treatments. Some effective antigenic properties even completely disappear. Although some antigen restoration techniques have been used, it is still difficult to reach the level before the treatment. This makes the immunohistochemical diagnosis of paraffi

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, a kind of configuration method of fixative for slices, formula ratio is calculated as follows by weight steps:

[0027] 10 parts of methanol, 10 parts of glutaraldehyde, 5 parts of ether, dilute the above substances to 100 parts with PBS buffer solution with a mass concentration of 5%; add 300g of sucrose to each 1L solution in the above solution, and mix thoroughly; after fully mixing, use 1mol / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH to 8.0.

Embodiment 2

[0028] Embodiment 2, a kind of configuration method of fixative for slices, formula ratio is calculated as follows by weight steps:

[0029] 20 parts of methanol, 25 parts of glutaraldehyde, 15 parts of ether, dilute the above-mentioned substances to 100 parts with PBS buffer solution with a mass concentration of 5%; add 500 g of sucrose to each 1 L of the above solution, and mix thoroughly; / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH to 9.3.

Embodiment 3

[0030] Embodiment 3, a kind of preparation method of fixative for slices, formula ratio is calculated as follows by weight steps:

[0031] 15 parts of methanol, 20 parts of glutaraldehyde, 10 parts of ether, dilute the above-mentioned substances to 100 parts with PBS buffer solution with a mass concentration of 5%; add 400g of sucrose to each 1L solution in the above solution, and mix thoroughly; after fully mixing, use 1mol / L sodium hydroxide or 1mol / L hydrochloric acid to adjust the pH to 9.

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Abstract

The invention relates to a method for preparing stationary liquid for slicing and application of the stationary liquid for the slicing, and belongs to the technical field of clinical medicine application. The method comprises the steps that methyl alcohol, glutaraldehyde and diethyl ether are mixed, and PBS buffer solutions are used for attenuation; saccharose is added to the solutions to be fully mixed, a pH value is regulated, and the stationary liquid for the slicing is obtained. Tissues are treated through pretreatment, saccharose placing, embedding, slicing and washing. The method is simple in working progress and step, the operating work amount of technical personnel is reduced, the operating steps of the whole process are few, needed time is shortened, tissues free of dehydration and waxdip can be kept fresh more highly, antigens for immunohistochemistry are kept complete and excellent, more accurate diagnosis conditions can be provided, xylene and other highly-polluted poisonous reagents are not needed, environmental pollution is reduced, and the guarantee for the body health of the technical personnel is enhanced.

Description

technical field [0001] The invention relates to a configuration method of a fixative for slices and an application thereof, belonging to the technical field of clinical application. Background technique [0002] Histopathological examination has been used in medical history for more than 100 years. Through pathological tissue biopsy examination, many diseases, especially tumors and other diseases, can be finally diagnosed. However, with the development of medicine, in routine tumor pathological diagnosis, there are many cases where it is difficult to make a clear morphological diagnosis only by H.E. staining. [0003] In recent years, with the development of clinical tumor diagnosis and treatment techniques, as well as the development of immunohistochemical techniques and the emergence of various specific antibodies, many difficult tumors have been more clearly identified through special immunohistochemistry. diagnosis. Especially for certain difficult tumors, the correct ...

Claims

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Application Information

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IPC IPC(8): G01N1/36
Inventor 吴天骥
Owner 吴天骥
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