Preparation of multi-epitope TK-1 antibody, and application of multi-epitope TK-1 antibody in evaluating treatment effect on tumor patient

A multi-epitope, antibody technology, applied in the direction of anti-enzyme immunoglobulin, egg-derived immunoglobulin, measuring device, etc., can solve the problems such as the need to improve the sensitivity, not suitable for physical examination screening, etc., to prolong survival and quality of life , easy commercial transportation, high sensitivity effect

Active Publication Date: 2012-06-27
SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Several teams have prepared different anti-TK1 antibodies for the identification and monitoring of tumo...

Method used

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  • Preparation of multi-epitope TK-1 antibody, and application of multi-epitope TK-1 antibody in evaluating treatment effect on tumor patient
  • Preparation of multi-epitope TK-1 antibody, and application of multi-epitope TK-1 antibody in evaluating treatment effect on tumor patient
  • Preparation of multi-epitope TK-1 antibody, and application of multi-epitope TK-1 antibody in evaluating treatment effect on tumor patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1: Selection of specific polyepitope antigens

[0046] Such as figure 1 As shown, the software predicts that TK1 has no signal peptide and no transmembrane structure. The diagram shows the distribution of 234 amino acids in the single-chain TK1 protein, indicating that the distribution of amino acids is relatively uniform. The map provides options: 1) From the perspective of antigenicity, it is better to select the peptide at the place where the blue curve peak is high; 2) According to the distribution of hydrophilic amino acids, select the hydrophilic region; 3) Select the negative value Aromatic amino acids with strong hydrophobicity.

example 2

[0047] Example 2: Select the antigen of high sensitivity, high specificity multi-site combined anti-human TK1-IgY antibody

[0048] Selection of antigens for the preparation of highly sensitive and highly specific multi-site combined anti-human TK1-IgY antibodies: Since the partial sequence of human cervical cancer TK1 has the same epitope as other proteins, the obtained antibodies may be different from other non-specific proteins There is cross-reaction and purification is difficult. According to the published and known TK1 sequence (protein library), select the special peptide exposed on the surface of the protein. According to the specificity of the polypeptide predicted by the software, the polypeptide fragments are listed in the following table. Antigen fragments with strong TK1 specificity were screened, and TK1 N-terminal 23 peptide, C-terminal 20 peptide and C-terminal 28 peptide were designed as haptens.

[0049]

[0050]

Embodiment 3

[0051] Example 3: Screening for specific polyepitope antigens

[0052] Selection of specific multi-epitope antigen: according to the well-known spatial structure of TK1, select the antigenic determinant exposed on the surface of TK1 protein, screen the antigenic fragment with strong immunity of TK1 according to Example 2, screen the N-terminal 23 peptide of TK1, and the C-terminal The 20 peptide and the C-terminal 28 peptide are antigen fragments with strong immunity. The amino acid sequences of the three segments are as follows (see sequence listing): 1) N-terminal 23 peptide (3-25): CINLPTVLPGSPSKTRGQIQVIL, 2) C-terminal 20 peptide (206 -225): CPVPGKPGEAVAARKLFAPQ, 3) C-terminal 28 peptide (198-225): AGPDNKENCPVPGKPGEAVAARKLFAPQ.

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Abstract

The invention provides a high-specificity high-sensitivity coordinated compound anti-human-TK1 antibody prepared from an antigenic determinant composed of human cervical cancer cell TK1 monomer N-terminal 23 peptide, C-terminal 20 peptide and C-terminal 28 peptide, and application thereof in tumor diagnosis. The antigenic determinant contains the following amino acid sequences: N-terminal 23 peptide (3-25): CINLPTVLPGSPSKTRGQIQVIL, C-terminal 20 peptide (206-225) CPVPGKPGEAVAARKLFAPQ, and C-terminal 28 peptide (198-225) AGPDNKENCPVPGKPGEAVAARKLFAPQ. The invention also relates to a method for preparing the antibody prepared from the antigen. The antibody kit provided by the invention has the characteristics of high sensitivity, high specificity, low cost and the like. The treatment effect on the tumor patient is evaluated by an enhanced chemiluminescent point blotting detection method, immunohistochemical detection and the detection kit.

Description

Technical field: [0001] The present invention relates to an antibody and its application, in particular to the preparation of a highly specific and highly sensitive multi-epitope TK1 antibody and its application in evaluating the therapeutic effect of tumor patients. Background technique: [0002] Cancer is the killer of human beings. Although there are many imaging detection methods and treatment methods, such as chemotherapy, endocrine therapy, radiotherapy and surgery, although great progress has been made, it is far from being perfect. There is the possibility of recurrence and metastasis. [0003] Cancer is a chronic disease in which cells proliferate abnormally. The abnormal and uncontrolled proliferation of cancer cells is caused by one or more mutations in the genes related to proliferation in normal cells. Therefore, an accurate understanding of the changes in the proliferation of tumor cells is the key to evaluating and analyzing the development direction of tumor...

Claims

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Application Information

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IPC IPC(8): C07K16/40C12N9/12C07K16/02G01N33/573G01N33/574
Inventor 周际李劲斯文·斯库格艾伦·何
Owner SHENZHEN HUARUI TONGKANG BIOTECHNOLOGICAL
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