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Multi-immunohistochemical analysis kit of lung cancer, and using method and application thereof

A technology of immunohistochemistry and analytical reagents, which is applied to the analysis of materials, biological tests, and material inspection products, etc., can solve the problems of being unable to effectively predict the effective inhibitory effect of immune checkpoint inhibitors on lung cancer, and achieve high accuracy and high detection efficiency , the effect of eliminating interference

Active Publication Date: 2019-04-09
ZHENYUE BIOTECHNOLOGY JIANGSU CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem that the prior art cannot effectively predict whether immune checkpoint inhibitors have an effective inhibitory effect on lung cancer, the present invention provides a multiple immunohistochemical analysis kit for lung cancer. According to the detection results of the kit, it can effectively To judge the effectiveness of immune checkpoint inhibitors, with high sensitivity and specificity

Method used

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  • Multi-immunohistochemical analysis kit of lung cancer, and using method and application thereof
  • Multi-immunohistochemical analysis kit of lung cancer, and using method and application thereof
  • Multi-immunohistochemical analysis kit of lung cancer, and using method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] 20 cases of lung cancer tumor tissues were selected: 10 cases each with poor efficacy of immunotherapy (shorter overall survival (OS) (1-10)) and good efficacy of immunotherapy (longer OS (11-20)). The survival period is shown in Table 1.

[0111] Table 1 Survival period of 20 lung cancer patients

[0112] sample number

[0113] Multiplex immunohistochemical analysis of immune cells and immune checkpoint molecular detection was performed as follows. 1 slice per patient, labeled with 6 molecules. The six molecules and their staining order are: CD163, CD68, PDL1, CD8, PD1, CD57. The primary antibody, secondary antibody, and fluorescent dye information during the experiment are shown in Table 2:

[0114] Table 2 Primary antibody, secondary antibody and fluorescent dye information

[0115] Antibody name

[0116] Example operation steps:

[0117] 1. Bake the paraffin sections in a 60°C incubator for 120 minutes;

[0118] 2. Dewaxing and hydration: x...

Embodiment 2

[0157] A kit for multiple immunohistochemical analysis of lung cancer, including monoclonal antibody group, antigen retrieval solution, horseradish peroxidase-labeled secondary antibody, hydrogen peroxide, fluorophore-labeled tyrosine salt and nuclear staining agent;

[0158] The monoclonal antibody group is: CD163 monoclonal antibody, CD68 monoclonal antibody, PDL1 monoclonal antibody, CD8 monoclonal antibody, PD1 monoclonal antibody and CD57 monoclonal antibody;

[0159] The fluorescent groups are 520-FITC, 540-AF517, 570-Cy3, 620-Cy3.5, 650-Cy5 and 690-Cy5.5;

[0160] The kit also includes an antiquencher, a washing agent and a blocking solution.

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Abstract

The invention provides a multi-immunohistochemical analysis kit of lung cancer, and a using method and application thereof, and relates to the field of the multi-immunohistochemical technology. The multi-immunohistochemical analysis kit defines that the monoclonal antibody group comprises more than two types of a CD163 monoclonal antibody, a CD68 monoclonal antibody, a PDL1 monoclonal antibody, aCD8 monoclonal antibody, a PD1 monoclonal antibody and a CD57 monoclonal antibody. The multi-immunohistochemical analysis kit uses the human or animal in vitro tissue, which is given the immune checkpoint inhibitor, as the sample to test, so as to judge the lung cancer effect validity of the multi-immunohistochemical analysis kit. The invention further provides a using method of the multi-immunohistochemical analysis kit; the method can effectively mark not less than 6 types of immune checkpoints on the same one tissue; and the cross reaction does not exist among multiple marks.

Description

technical field [0001] The invention relates to the technical field of multiple immunohistochemistry, in particular to a multiple immunohistochemical analysis kit for lung cancer and its use method and application. Background technique [0002] Lung cancer is the leading cause of cancer-related death globally and in China. In China, whether it is tumor incidence or mortality, lung cancer occupies the first place, seriously threatening people's health. [0003] Lung cancer is divided into small cell lung cancer and non-small cell lung cancer according to pathological classification, among which non-small cell lung cancer (NSCLC) is the main lung cancer subtype, accounting for about 85%. Advanced NSCLC is divided into squamous cell subtype and non-squamous cell subtype. Among them, the squamous cell subtype accounts for about 25% of NSCLC in the Western population, and about 30-46% of the NSCLC in the Chinese population (according to reports from different regions in China)....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577G01N33/58
CPCG01N33/57423G01N33/577G01N33/582
Inventor 张恒辉陈衍辉王雅婷罗红丽胡莹刘阳
Owner ZHENYUE BIOTECHNOLOGY JIANGSU CO LTD
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