Application of PSMD4 protein in preparation of liver cancer prognostic evaluation kit
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1. PSMD4, liver cancer prognosis technology, applied in the biological field
Inactive Publication Date: 2013-05-08
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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[0007] So far, there is no literature reporting that PSMD4 is associated with HCC recurrence or prognosis
Method used
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Embodiment 1
[0052] The present invention adopts immunohistochemical method to detect the relative expression of PSMD4 protein in liver cancer tissue, and the specific steps are:
[0053] (1) Paraffin sections of liver cancer tissues were prepared, and the oven was set at 60° C. overnight;
[0056] (3) 3%H 2 o 2 Methanol solution, place at room temperature for 20 minutes;
[0057] (4) Wash with double distilled water for 5min×3;
[0058] (5) Antigen retrieval: Put slices into 0.01M citrate buffer (pH6.0) and boil for 5 minutes, stop fire for 10 minutes, and boil for 5 minutes;
[0059] (6) Naturally cool to room temperature, wash with double distilled water for 5min×3;
[0060] (7) Block with 1% BSA for 30 minutes at 37°C;
[0061] (8) Shake off the blocking solution, without washing, add primary...
Embodiment 2
[0075] Sample: a tissue section of a liver cancer patient's tumor, and the relative expression of PSMD4 protein in the liver cancer tissue was detected by the above-mentioned immunohistochemical method test steps. Micrographs of liver cancer tissue and paracancerous tissue image 3 shown. After calculation, the immunohistochemical score of the tissue was 0.478609.
[0076] According to postoperative follow-up, the patient died 3.5 months after operation, and the disease-free survival period was only 1.5 months.
Embodiment 3
[0078] Sample: a tissue section of a liver cancer patient's tumor, and the relative expression of PSMD4 protein in the liver cancer tissue was detected by the above-mentioned immunohistochemical method test steps. Micrographs of liver cancer tissue and paracancerous tissue Figure 4 shown. After calculation, the immunohistochemical score of the tissue was 0.291412.
[0079] According to postoperative follow-up, the patient is alive and well without recurrence.
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Abstract
The invention belongs to the biotechnology field, relates to application of PSMD4 protein, and in particular relates to application of the PSMD4 protein in preparation of a liver cancer prognostic evaluation kit. The invention adopts an immunohistochemistry technology, a microscope photograph technology and computer image processing software to test the relative transcript level of PSMD4 protein of tumor tissues relative to the tumors and determine the correlation between the relative transcript level of PSMD4 protein and the prognosis of hepatocellular carcinoma patients after operation by a manner of combining the postoperative follow-up information. The PSMD4 protein can be used for preparing a protein molecular marker for judging prognosis of the liver cancer patients, and has important instruction significance to postoperative monitor and sequential therapy of the hepatocellular carcinoma patients.
Description
technical field [0001] The invention belongs to the field of biotechnology and relates to an application of PSMD4 protein, in particular to the application of PSMD4 protein in the preparation of liver cancer prognosis assessment kit. Background technique [0002] Liver cancer is one of the most common malignant tumors, and its morbidity and mortality have remained high for a long time. my country is the country with the most incidence of liver cancer in the world, accounting for about 50% of the world's new cases every year. Statistics show that the high metastatic recurrence rate after liver cancer surgery (5-year recurrence rate reaches 70%, small liver cancer also reaches 50%) has become a bottleneck for further improving long-term efficacy, and there are few indicators for judging the prognosis of liver cancer. [0003] Liver cancer metastasis is a complex process involving and interacting with multiple factors, which is not only related to the cancer cells themselves, ...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574
Inventor 王红阳丁劲孙文李恒宇王雪向代民
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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