Method for rapidly removing three viruses of lily

A lily and virus technology, applied in the biological field, can solve problems such as low sensitivity, and achieve the effects of improved detoxification rate, improved survival rate, and easy cutting

Inactive Publication Date: 2010-02-03
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the following problems still exist in the existing detection methods: stem (root) tip culture (stem or shoot tip culture) is an aseptic culture in which the apex part of the stem (root) or the stem (root) tip meristem part is cut.
At the same time, related to the virus detection technology and method, indicator plants and ELISA were used for detection in the early stage, and the sensitivity was low, and the virus was not completely removed in many cases.

Method used

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  • Method for rapidly removing three viruses of lily
  • Method for rapidly removing three viruses of lily
  • Method for rapidly removing three viruses of lily

Examples

Experimental program
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Embodiment Construction

[0016] 1) Detoxification method

[0017] Take the bulb that has sprouted 3-6cm after low temperature treatment, cut it from the stem node, and remove the outer scales. Rinse with tap water for 1 hour, soak in distilled water for 10 minutes, disinfect with 0.1% mercuric chloride for 10 minutes (add a few drops of Tween 80), and finally rinse with sterile water for 3 to 5 times. Under aseptic conditions with a stereo microscope (Olympus, SZX10) with a micrometer, cut out shoot apex meristems of different sizes with 1-2 leaf primordia, and quickly inoculate the medium with different concentrations of ribavirin , Carry out shoot tip culture. The medium components are: MS+IBA1.0mg / L+NAA0.1mg / L, sucrose 3%, agar 6g / L. The culture condition is 2000~3000lx, the light time is 14h / d, and the culture temperature is 20~25℃.

[0018] According to the uniform experiment design, the shoot tip size is set to 4 levels 0.2-0.4mm, 0.5-0.7mm, 0.8-1.0mm, 1.0-1.2mm; ribavirin concentration is set to ...

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Abstract

The invention relates to a method for rapid removing three sorts of virus in lily, belonging to biologic technical field, which is characterized in that: the meristematic tissue of stem tip is inoculated in the culture medium with ribavirin; wherein, the size of the stem tip is 0.5 to 0.8mm, and the concentration of the ribavirin is 4 mg / L to 6 mg / L. The invention has the advantages of high detoxication rate, high survival rate and rapid regeneration speed.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly removing three lily viruses. Background technique [0002] The three types of lily viruses, LSV, CMV, and LMoV, are the main reasons for the decrease in lily cash crops. The current main methods for removing the above three viruses are as follows: 1) Stem apex meristem method: Stem apex meristem is the area with the least virus content. Stem apex meristem is limited to the apical cone area, and its length is not more than 0.1mm for aseptic culture. However, such a small shoot tip is actually difficult to obtain, the survival rate is very low, and the cultivation time for seedlings is also very long. Therefore, in actual cultivation, growth cones with leaf primordia are often used for cultivation, usually 0.2-0.4mm (millimeters) of shoot apex meristems, but the probability of being poisonous increases. Kim detoxified the stem tip of Lily ‘Georgia’ with LSV, CMV, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00A01C1/00A01H4/00
Inventor 明军徐榕雪刘春穆鼎汤庚国王晓武刘博
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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