Method for repeatedly taking blood of tree shrew by cardiocentesis

Abstract

The invention relates to a method for repeatedly taking blood of a tree shrew by cardiocentesis. The method comprises the following steps: (1) fixing the tree shrew to a table top in a horizontal supine position, and keeping the chest flattened; (2) ensuring that the depth, that the needle for taking blood can penetrate into the chest cavity of the tree shrew, does not exceed 5 mm; (3) slightly touching the chest of the tree shrew to determine the position with the strongest systole vibrations, and inserting the needle from the outer wall of the right chest of the tree shrew to the left chest, and taking blood when the blood automatically flows into the injector; (4) taking the blood of the tree shrew once a week, wherein the blood taking amount for each time is controlled within 2 mL; non-discontinuously taking the blood, wherein the blood taking amount for each time is controlled within 3 mL; (5) after taking the blood by cardiocentesis, putting the tree shrew in a prepared black cloth bag to quietly rest for 5 minutes, and putting the tree shrew into the rearing cage; and after taking the blood by cardiocentesis, controlling the illumination in the rearing room at 30-50 LX within 3 days, and feeding 2 g of fresh meet to each tree shrew every day, so that the tree shrew for taking blood can recover as quickly as possible. The invention can be used for taking blood in a safe and efficient way. The time for taking the blood of one tree shrew is only 5-10 seconds. The tree shrews can not die after repeated taking blood for more than ten times.

Description

technical field [0001] The invention relates to a method for repeated blood collection by heart puncture in tree shrew experiment process. Background technique [0002] With the continuous improvement of the artificial domestication and breeding technology of tree shrews, and the deepening of the research on the biological characteristics of tree shrews, the animal models that use tree shrews to replicate human diseases will become more and more extensive. During the tree shrew experiment, repeated blood collection is required for relevant testing. At present, the main methods of tree shrew blood collection in various laboratories include: orbital plexus blood collection, femoral artery (vein) blood collection, tail vein blood collection, and cardiac puncture blood collection. The first three methods are: Methods The blood collection volume is small and time-consuming, and the blood collection equipment must be treated with anticoagulants, which is prone to hemolysis and aff...

AI Technical Summary

Problems solved by technology

During the tree shrew experiment, repeated blood collection is required for relevant testing. At present, the main methods of tree shrew blood collection in various laboratories include: orbital plexus blood collection, femoral artery (vein) blood collection, tail vein blood collection, and cardiac puncture blood collection. The first three methods are: The method takes a small amount of blood and takes a long time. The blood collection equipment must be treated with anticoagulants, which is prone to hemolysis and affects the accuracy of the test results.

Cardiac puncture blood collection can obtain the maximum blood volume, save time, but the technical essentials are not easy to master, which may easily cause the death of tree shrews