Immunohistochemical glass slide with internal reference and its preparation method and application

Abstract

The invention discloses an immunohistochemical glass slide with an internal reference, which comprises a label mark zone, a to-be-tested sample paster zone and an internal reference mark zone, whereinthe internal reference mark zone is fixed with a cellular immunity chip containing at least three positive expression internal references and a negative expression internal reference; the at least three positive expression internal references comprise cell strains capable of stably expressing that the corresponding antigen intensities of target antibodies are weak positive staining, intermediatepositive staining and strong positive staining; the negative expression internal reference is the cell strain for not stably expressing corresponding antigens of the target antibodies. The immunohistochemical glass slide can correct objective operation error, intragroup experimental error and subjective defect of interpretation, and others existed in the existing immunohistochemical technique, andalso can discriminate the difference of the dyeing intensity, and can perform the quality control on the full process of the experimental detection and result report; thus the correctness and accuracy of the immunohistochemical experimental detection are guaranteed. The invention further provides a preparation method and an application of the immunohistochemical glass slide with the internal reference.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an immunohistochemical slide with an internal reference and a preparation method and application thereof. Background technique [0002] Immunohistochemistry (IHC) uses the principle of specific binding of antigens and antibodies to determine whether there is a target antigen in the tissue cells by chemical reaction to develop the color of the chromogenic agent labeled on the antibody, and to locate, qualitative and quantitative it. The experimental detection technology studied, the staining results are an important basis for clinical pathologists to make a correct diagnosis, especially in the pathological diagnosis of tumors, differential diagnosis, screening of tumor targeted therapy drugs and prognosis evaluation, etc., which are of great significance. At the same time, it also provides important basis and great convenience in scientific research work. [0003] Due to th...

AI Technical Summary

Problems solved by technology

[0005] The objective defects of the above-mentioned immunohistochemical experiments are: ①Even if they are qualified after training, there are objective differences in the specific operations among different experimental technicians

Even if the same person repeats the same immunohistochemical experiment at different times, there are objective differences

②Objectively, it is difficult to achieve the same experimental conditions for any two repetitions of the same experiment

However, the setting of such an internal reference within the group still cannot eliminate the experimental differences within the group. More importantly, such a qualitative internal reference setting has no effect on the screening of differences in the staining intensity of immunohistochemical experiments caused by experimental operations, etc. clear guiding value

[0006] 2) Subjective defects in interpretation

① For a long time, the results of immunohistochemistry have been interpreted subjectively by clinical pathologists under the microscope. Different pathologists have subjective incomplete consistency in the judgment scale of immunohistochemical staining intensity; even if the same pathologist There may also be some differences when doctors interpret the same immunohistochemical staining slide after a long time interval

②If the intensity of immunohistochemical staining is increased or decreased due to the error of the experimental system, clinical pathologists will not be able to identify it when reading and interpreting the slides

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