41 results about "Auto immunite" patented technology

An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte-dependent product, and a detection zone located downstream at which the detectable product can be observed. By controlling movement of the support to its first and second positions, the cassette can effectively handle a variety of samples having different flow rates.

The present invention relates to relates to an amyloid ss peptide analogues comprising an amino acid sequence or a peptidomimetic thereof, wherein the sequence (i) forms a loop, (ii) has at least 66 % identity to the amino acid sequence of native Ass peptide or a portion thereof, (iii) comprises at least 6 contiguous amino acid residues and (iv) has at least 2 non-contiguous amino acid residues which are covalently linked with each other, oligomers comprising a plurality of said amyloid ss peptide analogues, processes for preparing the amyloid ss peptide analogues or oligomers, compositions comprising the amyloid ss peptide analogues or oligomers, and uses of the amyloid ss peptide analogues or oligomers such as their use for treating or preventing an amyloidosis (e.g.; by active immunization), for diagnosing an amyloidosis, and for providing agents that are capable of binding to the amyloid ss peptide analogues or oligomers. The subject invention also describes agents that are capable of binding to the amyloid ss peptide analogues or oligomers, e.g. antibodies, compositions comprising the agents, and uses of the agents such as their use for treating or preventing an amyloidosis (e.g. by passive immunization) and for diagnosing an amyloidosis.

The invention relates to an automatic immunity analyzer which comprises a rack, a main control module, at least one cup body transfer module, at least one sample adding module, at least one reagent adding module and at least one sampling detection module, and a plurality of station bins are arranged on the rack; the cup body transfer module is used for driving the reaction cups to transfer among the station bins; the sample adding module comprises a sample needle for adding a sample; the reagent adding module comprises a reagent needle for adding a reagent; the sampling detection module comprises a detection needle used for extracting a sample and a detection assembly connected with the detection needle. By adopting an assembly line type structure, integrated operation of sample adding, incubation, reagent adding, uniform mixing, cleaning and detection can be realized, the automation degree is relatively high, the operation difficulty is reduced, and the detection efficiency is improved.

The invention relates to a full-automatic immunoblotting instrument and a detection method. The full-automatic immunoblotting instrument comprises a rack, wherein kits, a reagent filling device, reaction vessels with membrane strips, sample boxes, and a sample filling mechanism used for adding sample liquid in the sample box into the reaction vessel are arranged on the rack. A large disc and a small disc which are coaxial are rotationally arranged on the rack; the plurality of kits are arranged on the small disc along the circumferential direction of the small disc; the reagent filling deviceis positioned at the outer edge of the small disc; a plurality of reaction vessels and a plurality of sample boxes are arranged in the circumferential direction of the large disc; the sample boxes arearranged on the edge of the large disc; the reaction vessels are installed between the center of the large disc and the sample boxes, and the sample filling mechanism is arranged on a short-stroke rail and moves in a radial direction of the large disc. The space structure of the large disc and the small disc is reasonably and efficiently utilized, the whole instrument tends to be miniaturized, efficient and automatic, a disposable suction nozzle is clamped, and cross infection is avoided.

Cell based assays are required for the detection of biological activity and hence are required for use as potency assays, detection of neutralizing antibodies and detection and quantification of the effector cell function of therapeutic antibodies or the quantification of the potency or neutralizing antibody response to virus vectors such as AAV vectors used in gene therapy. Cell-based assay are also required for the quantification of antibody mediated effector functions including complement-dependent cytotoxicity (CDC), antibody-dependent cellular phagocytosis (ADCP), and antibody-dependent cell-mediated cytotoxicity (ADCC). Cell based assays are difficult to adapt for use on automated immuno-assay platforms. Reporter-genes that encode readily visible proteins that can be quantified using fluorescence or luminescence such as luciferases (firefly, Renilla, Gaussia, Nano Luciferase etc), fluorescent proteins such as green fluorescent protein (GFP) or dsRED or an enzyme such as chloramphenicol acetyltransferase (CAT) or a protease and respond to signal transduction, that is directly related to the mechanism of action of a drug, can be used to quantify the potency of a drug following binding of a drug to a soluble target or cell surface target (receptor or other cell-surface molecule), anti-drug neutralizing antibodies, and therapeutic antibody induced effector cell function. The principal of the invention is that reporter-gene product or by-product produced either during the course of the cell-based assay or onconclusion of the cell based assay is quantified either in the cell medium or cell supernatant, for a secreted protein, or following lysis of the cells with a suitable passive lysis buffer. The reporter-gene product such as firefly luciferase is then detected in the cell medium or cell supernatant or cell lysate using an antibody pair (monoclonal or polyclonal) specific for the gene product such as a firefly luciferase labelled with the dual detection system specific for an ELISA or a particular automated assay platform such as Meso Scale Discovery electro-chemiluminescence (MSD-ECL), Luminex, SMC, Alpco, AlphaLISA, Gyros or label free detection using SPR such as the Biacore platform. The expression of the reporter-gene product such as firefly luciferase can be normalized with respect to the expression of a second reporter gene product such as Renilla luciferase or Nano Luciferase under the control of a constitutive promoter.

The present invention relates to a method for enhancing immunoreactivity of a tissue or cell sample fixed in a fixing medium, a target retrieval composition and its use. The method comprises providing a carrier in a horizontal position, said carrier having thereon a tissue or cell sample, said tissue or cell sample being on top of the carrier; contacting substantially the tissue or cell sample side of the carrier with a buffered target retrieval solution, wherein the target retrieval solution remains otherwise exposed to the environment; heating the tissue or cell sample and the target retrieval solution to a temperature above 100° C. The invention furthermore concerns the automated immunohistochemical staining of said samples, in particular the reactivation of antigens masked by fixation.

The invention belongs to the technical field of motor drive, and discloses a high-speed centrifugal turntable of an automatic immune analyzer and a positioning control method, comprising a centrifugal turntable, a motor driver, a brushless DC motor, a reagent card slot and an absolute encoder; the quality of the centrifugal turntable is concentrated in At the central axis, the outer edge is a fan-shaped strip structure to minimize the mass and reduce the moment of inertia under the condition of a certain radius; the DC brushless motor accelerates the open-loop operation according to the S-shaped curve to a specific speed, which drives the direct-connected installation. The centrifugal turntable on the motor output shaft rotates to separate the samples in the reagent card slot. After the separation is completed, the motor speed is reduced. When the speed is reduced to the set speed, the motor moves in a closed loop. The device detects the position of the centrifugal turntable, and uses the PID control algorithm to precisely control the centrifugal turntable to stop at the specified position, which improves the positioning accuracy of the high-speed centrifugal turntable of the automatic immunoassay analyzer and facilitates the subsequent processing of the sample by the robotic arm.

An instrument for detecting signal from a biological sample includes a pipettor module configured to hold a plurality of pipettes in respective pipette positions, to hold liquid in one or more pipette tips, and to pipette liquid in and out of the one or more pipette tips. Each of the one or more pipette tips has a pipette tip point. The instrument further includes one or more magnets positioned such that each of the one or more pipette tips is adjacent one of the one or more magnets.

The invention belongs to the technical field of automatic control of medical equipment, and discloses a motor control system and a medical detection system of a fully automatic immune analyzer, including: a host computer for collecting control information; a master control card for receiving and analyzing the host computer The control information sent controls the precise movement of the motors of each module; the power distribution management and bus monitoring module is used to integrate the data line with the communication line, monitor and upload the running status in real time; the sample adding module is used to realize medical clinical testing. Reagent sample addition operation; centrifugation module, used to realize high-speed centrifugation of reagents in medical clinical testing; temperature control module, used to realize constant temperature incubation of reagents in medical clinical testing; dual robotic arm cooperative control module, used to realize medical clinical testing Reagent removal card puncture operation. The invention makes the wiring of the system simple and compact, eliminates accumulated errors, improves the precision of motor control, and improves the efficiency and positioning precision of the automatic immune analyzer.

The invention relates to an automatic immunoblotting analyzer, comprising: a reagent module, a sample injection module, a material addition module, a cleaning module, an incubation module, a liquid injection and suction module, an interpretation module, and a drying module. At the same time, a fully automatic immunoblotting analysis test method is also provided. The beneficial effects of the present invention are as follows: with the incubation module as the core, the other modules are arranged around the incubation module, according to the tested items, the sample injection module, the reagent module and the feeding module are used to realize automatic sample feeding and automatic addition of reagents, and the cleaning module is used to The feeding module is cleaned to avoid contamination between the agents. The incubation module is used to inject the cleaning solution and the waste liquid is removed. The drying module is used to dry the incubation module. Image analysis and result output do not require manual participation in the test process, and each module cooperates with each other to realize the whole process from sample injection to result output.

The invention discloses a microsphere-free homogeneous chemiluminescence system of biotin-avidin or streptavidin, and a method for quantitatively measuring antigens or antibodies, and relates to the technical field of chemiluminescence systems. The microsphere homogeneous chemiluminescence system includes: biotin-labeled antibody or antigen, 9,10-dihydroacridine-labeled antibody or antigen, HRP-labeled avidin or streptavidin. Biotin-avidin or biotin-streptavidin No Microspheres Honogeneous Luminescent (Biotin-Avidin / streptavidin) system does not use microspheres, it is a homogeneous system; the introduction of biotin ‑Avidin / streptavidin system can greatly improve chemiluminescence efficiency and detection sensitivity. The minimum detection concentration of S100B protein in the example can reach 0.015ng / ml; the detection process of this system does not need cleaning, is convenient and simple, and can be used in automatic Immunohomogeneous chemiluminescence system, POCT and microfluidic chips, etc. for rapid quantitative analysis.

The present invention relates to an automated immunoassay device and method. The automated immunoassay device comprises: a cartridge (13) in which a T tip tube (9), a reactive tube (11), a washing tube (3), and a signal measuring tube (5) are integrally connected to one another or separately arranged; a T tip (7) and a magnetic rod (31) which enter the multiple tubes (3, 5) sequentially to allow the migration of large magnetic particles (m), a capturing material, a signal material, and an analysis material by magnetic force, with the concomitant performance of reactions and washing processes;a cartridge holder (15) on which the cartridge (13) is stably placed; and a heating member (17) disposed in an area in which the reactive tube (11) is stably placed on the cartridge holder (15) so asto generate heat, wherein the large magnetic particles (m) comprise morphologically identical or different magnetic particles to which a material capable of capturing only a specific analysis materialper each of the morphologically different magnetic particles is coupled.

The invention provides a semi-automatic immunofluorescence analysis system, which includes a test card delivery device, an optical path structure, and an optical analysis device including the optical path structure. The invention has high sensitivity and accuracy. By shortening the distance between the convex lens and the detection area, the fluorescence with a larger angle range can enter the detection optical path, thereby improving the detection sensitivity and accuracy of the system. The structure is exquisite. The invention has the advantages of compact structure, good light density, high reliability, convenient assembly, and high-efficiency detection at low cost. It realizes semi-automatic accurate monitoring and fills the gap in this field.

The invention discloses a method for batching and scheduling mixed optimization of fully automatic immune testing equipment, comprising the following steps: S1, reading the information of the blood sample to be tested, reading the information of the sub-testing machine in the equipment, and reading the information of the item to be tested from the database Inspection step information; S2. Establish a hybrid optimization model of sub-batch division and inspection sequence scheduling based on the data obtained in step S1; S3. Perform hybrid optimization based on the multi-population cooperative migratory bird migration algorithm for sub-batch division and inspection sequence scheduling to obtain the total inspection time The shortest sub-batch division sequence and inspection order sequence; S4, generate a specific scheduling plan according to the sub-batch division sequence and inspection order sequence; S5, convert the scheduling plan into an operation instruction sequence for several sub-inspection machines in the equipment, so that the equipment in the equipment All inspection machines are inspected under unified scheduling. The invention calculates optimal sub-batch division through migratory bird migration algorithm, improves the flexibility of inspection, and improves the efficiency of batch inspection.

The invention relates to a full-automatic immunoblotting instrument which comprises a protective shell and further comprises a material injection unit, a reaction unit and a control unit, wherein the material injection unit is used for conveying a reagent to the reaction unit or discharging the reagent in the reaction unit; the reaction unit comprises a reaction chamber, and reagents in the reaction unit react or are combined in the reaction chamber; the control unit is electrically connected with the material injection unit and the reaction unit and is used for controlling the working states of the material injection unit and the reaction unit; the material injection unit, the reaction unit and the control unit are fixedly connected with the protective shell. The full-automatic immunoblotting instrument has the effects of reducing the test risk of testers, reducing the test difficulty of the testers and improving the working quality of the testers.