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Pathology aided analysis method based on immunohistochemical technique

An auxiliary analysis and immunohistochemical technology, which is applied in the field of pathological auxiliary analysis based on immunohistochemical technology, can solve the problems of low efficiency and high labor cost, and achieve the effect of reducing labor cost

Inactive Publication Date: 2017-06-13
KONFOONG BIOTECH INT
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  • Application Information

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Problems solved by technology

[0003] At present, pathologists in this field perform immunohistochemical diagnosis mainly by manual visual identification and counting. For pathologists who need to read a large number of slides, manual counting of immunohistochemical positive cells is a very large task, making immunohistochemical The labor cost of diagnosis remains high and the efficiency is not high

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0022] In a preferred embodiment, as figure 1 As shown, a pathological auxiliary analysis method based on immunohistochemical technology is proposed, which may include a preset step: using a pathological scanner to scan the pathological section processed by immunohistochemical technology to obtain a pathological digital image;

[0023] Can also include:

[0024] Step S1, inputting the pathological digital image to obtain a cell sample interval;

[0025] Step S2, setting a parameter set and searching for cells conforming to the parameter set in the cell sample interval as target cells;

[0026] Step S3, respectively counting the number of positive cells and negative cells in the target cells with two classifications of positive cells and negative cells according to the staining color of the target cells;

[0027] In step S4, the ratio of positive cell...

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Abstract

The invention relates to the technical field of biology, in particular to a pathology aided analysis method based on an immunohistochemical technique. The method can comprise a preset step that a pathological section treated through the immunohistochemical technique is scanned through a pathology scanner to obtain a pathology digital image, and further can comprise the steps that 1, the pathology digital image is input to obtain a cell sample interval; 2, a parameter set is set, and cells conforming to the parameter set are sought in the cell sample interval to serve as target cells; 3, the number of positive cells and negative cells in the target cells with two kinds of classification of the positive cells and the negative cells are counted according to dyed colors of the target cells; 4, a proportion value of the positive cells in the target cells is obtained according to the number of the positive cells and the negative cells, and the proportion value is output. The method has the advantages that a doctor can be assisted in conducting pathology diagnosis on the basis of the immunohistochemical technique, and the labor cost of immunohistochemical diagnosis is lowered.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a pathological auxiliary analysis method based on immunohistochemical technology. Background technique [0002] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody, through chemical reaction to make the chromogenic agent (fluorescein, enzyme, metal ion, isotope) of the labeled antibody develop color. Determination of antigens (polypeptides and proteins) in tissue cells, and their localization, qualitative and relative quantitative research are called immunohistochemical techniques or immunocytochemical techniques. [0003] At present, pathologists in this field perform immunohistochemical diagnosis mainly by manual visual identification and counting. For pathologists who need to read a large number of slides, manual counting of immunohistochemical positive cells is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06F19/00
CPCG06T7/0012G06T2207/30024G06T2207/30242G16H50/20
Inventor 刘炳宪谢菊元王焱辉王克惠许利庆
Owner KONFOONG BIOTECH INT
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