40results about How to "Real-time dynamic detection" patented technology

The invention relates to a rare earth upconversion nano fluorescent probe and preparation and an application thereof. Heterogeneous functional fluorescent nanoparticles are formed from upconversion fluorescent nanoparticles and Au nanoparticles; the molar ratio of the upconversion fluorescent nanoparticles NaYF4:Yb<3+>/Er<3+> to Au is 5:1, 10:1, 15:1 or 20:1, and stirring is carried out for 2.5 h at the temperature of 140 DEG C; compared with the upconversion fluorescent nanoparticles, the obtained particles have red fluorescence enhanced and green fluorescence decreased. The rare earth upconversion fluorescent nanoparticles are good in photochemical stability and low in toxicity; and moreover, a near-infrared laser is used as an excitation light source, the rare earth upconversion fluorescent nanoparticles have strong optical penetration ability, have no background fluorescence, and have potential application value in the fields of biological fluorescence imaging and CT developer.

The invention relates to an online detecting device and online detecting method for synchronous belt type corn kernel breaking rate. The device comprises: a detecting box, a blanking unit, a mechanismexecuting unit and a detecting unit, wherein the blanking unit comprises a blanking slot and a blanking chute, the mechanism executing unit is in a synchronous belt transmission structure, and the detecting unit comprises a CCD industrial camera, a processing chip, and an in-vehicle control and display terminal. Each of the units cooperates with each other to transmit data in real time to ensurethe smooth online detection of the corn kernel breaking rate. The method comprises the following steps: the CCD industrial camera collects corn kernel images and outputs the images to the processing chip for processing to identify broken corn kernels, and the in-vehicle control and display terminal calculates a corn kernel breaking rate. The device provided by the invention is small in size, simple and reliable in structure, convenient to carry, easy to be installed with the combine harvester grain box, high in clarity of images acquired by the CCD industrial camera, simple and efficient in algorithm, and fast in image data information processing speed, so that advantages of high detection efficiency, strong reliability and high precision are obtained.

The present invention is one kind of high flux multiparameter image surface plasma resonance test instrument, and relates to detection technology. The test instrument in synchronous scanning structure has a triangular optical prism, an array surface plasma resonance chip, a micro measuring pond with at least one flow channel, an automatic sampling system communicated with the micro measuring pond, an optical system, a mechanical scanning system, a data acquiring system, an embedded microprocessor, and a computer with information acquiring and processing software and system operating software for controlling the test instrument. It operates in one of three operation modes to complete quantitative high flux multiparameter detection.

The invention discloses a method and device for measuring dynamic viscosity based on light suspended particles. The method comprises stably capturing particles in a sample to be tested through a capturing beam, applying a modulated beam to the particles in the trapping well, regulating the optical power of the modulated beam by an excitation signal so that the particles carry out response motion after the action of the modulated beam on the particles, acquiring a real-time motion curve of the particles through detecting the optical power change of the scattered light of the captured beam, andcarrying out parameter fitting on the motion curve to obtain resonance frequency and viscous damping so that the dynamic viscosity of the sample to be detected is obtained. The device comprises a sample module, a capture optical trap module, an excitation modulation module and a motion detection module. The method adopts the optical non-contact method to measure the dynamic viscosity of the sample, has a high measurement precision and a fast response speed, and can dynamically detect the dynamic viscosity change of the sample under different temperature and pressure conditions in real time.

The invention relates to a detecting system capable of accurately acquiring ECL (electrochemiluminescence) spectral information. The detecting system comprises an electrochemistry equipment unit, an ECL radiation generation device unit, a spectrum detection device unit and a data processing and control unit, wherein the ECL radiation generation device unit comprises an ECL cell as well as a working electrode, a reference electrode and a counter electrode which are connected with the electrochemistry equipment unit; the spectrum detection device unit comprises a collection objective lens and a CCD grating spectrometer; the ECL radiation generation device unit and the collection objective lens are arranged in a cassette, a luminous surface of the working electrode faces the collection objective lens, and the focus of the collection objective lens coincides with an entrance slit of the CCD grating spectrometer; the cassette is directly connected with a fixing plate of the entrance slit, and a detecting system light path is isolated from the outside; the CCD grating spectrometer and the electrochemistry equipment unit are triggered through TTL (transistor transistor logic) signals to realize synchronous operation, and the acquired electrochemical signals and ECL spectrum signals are synchronously input into the data processing and control unit.

The invention discloses a device based on a micro-nano fluid control reflection interference spectral imaging system and a realization method. The device comprises a micro fluid pump, a micro fluid control sample cell system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection light source, and a computer. The C-port optical path switcher is connected to the positioning light source, the CCD sensor, and the spectrometer through optical fibers; the CCD sensor and the spectrometer are both connected to the computer, and the computer software collects detection data and analyzes the results. According to the system, the object lens of the microscope is taken as the optical probe; compared with the conventional method that takes a detector as the probe, the system has the advantages that a fixing device for fixing a probe is not needed, the optical path integration degree of the system is improved; moreover, an imaging and positioning system is used to obtain the specific position of a sample, and thus the detection precision is improved. The morphology feature imaging of samples and reflection interference spectrum collection can be switched in real time, and thus the in-situ dynamic realtime detection of an analysis sample can be realized.

The invention provides an immunofluorescence kit for detecting PD-L1 gene expression of an esophageal squamous cell carcinoma patient through peripheral blood circulating tumor cells, and belongs to the field of molecular biology, and particularly the kit comprises a primary antibody suspension composed of goat serum, mouse anti-CK, mouse anti-CD45 and rabbit anti-PD-L1 genes, and a secondary antibody suspension composed of a fluorescently-labeled goat anti-mouse, a fluorescently-labeled goat anti-mouse and a fluorescently-labeled goat anti-rabbit. The detection method mainly comprises the following steps: collecting peripheral blood, treating the peripheral blood, filtering and enriching the circulating tumor cells, and detecting the PD-L1 gene expression condition of the circulating tumor cells by an immunofluorescence method.

The invention relates to the technical field of biology, provides a kit and a method for screening prostate cancer and aims at solving the risk assessment problem of the prostate cancer. The kit for screening the prostate cancer, provided by the invention, comprises an ERG primer and a probe thereof, an SPDF primer and a probe thereof, an MAX primer and a probe thereof, an IL32 primer and a probethereof as well as a PDGFA primer and a probe thereof; and an internal control is an SPK primer and a probe thereof. By adopting the kit, a specimen is collected by adopting a non-invasive means, real-time dynamic detection can be performed, and the detection accuracy is high.

The invention provides a kit for detecting PD-L1 gene mutation of peripheral blood circulating tumor cells of a prostate cancer patient and a detection method. The kit comprises a diluent, a destaining , a staining solution A, a staining solution B, an anti-human PD-L1 primary antibody, an enzyme-labeled goat anti-human secondary antibody, 0.1% of Triton X-100, 0.3% of H2O2 and a reagent A. The detection method comprises the following steps: separating and acquiring peripheral blood circulating tumor cells (CTC) by using a membrane filtration device, and detecting the PD-L1 expression condition of the peripheral blood CTCs by using an immunohistochemical technology. By utilizing the detection method provided by the invention, the PD-L1 expression condition of a patient with advanced or recurrent hepatocellular carcinoma can be detected without acquiring a tissue specimen through needle biopsy, and real-time dynamic detection can be realized by utilizing a minimally invasive technology;by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by the edge effect possibly generated in the dyeing process is avoided, the stability is good, the cell loss is reduced, and the detection accuracy is improved.

The invention discloses an in-acupoint in-vivo dynamic electrical-characteristic detecting device. The detecting device comprises an upper computer and a lower computer. The upper computer is connected with the lower computer through a serial communication module. Two electrode wires of the detecting device are led out to be connected with handles of acupuncture needles. When the detecting device is started to detect acupoint resistance, the lower computer transmits collected data to the upper computer, and real-time dynamic oscillogram display is realized. The detecting device has the advantages that in-vivo, real-time and dynamic detection of the acupoint resistance is realized, and detection results are stable by constant-flow-source detection.

The invention discloses a double-electrode acupuncture needle which comprises a needle body and a needle handle. An insulating layer is wrapped on the needle body being 0.3cm away from a needle tip, an electroconductive layer is coated on the insulating layer, an electrode leading-out line is bonded on the electroconductive layer and then covered by an insulating sleeve to serve as a first electrode, the needle handle serves as a second electrode, and the two electrodes are combined on an acupuncture needle. The double-electrode acupuncture needle has the advantages that in-vivo, realtime and dynamic detection of acupoint electrical impedance is realized, conventional influencing factors in the process of detection are avoided, and synchronousness of acupuncture and detection is realized.

The invention discloses an unmanned aerial vehicle-mounted pavement detection system and detection method, and the system comprises an unmanned aerial vehicle which is provided with a GPS system and an unmanned aerial vehicle flight height control system. A camera is carried on the unmanned aerial vehicle and is connected with an image sensor; the output end of the image sensor is connected to theinput end of the signal amplification circuit, and the output end of the signal amplification circuit is connected to the input end of the signal conversion circuit; the output end of the signal conversion circuit is connected to a wireless data transmission radio station; the input end of the signal amplification circuit is connected with the output end of the signal detection and processing circuit, and the input end of the signal detection and processing circuit is connected with the controller. The ground wireless data transmission radio station is connected with the ground server throughthe level adapter plate, and the wireless data transmission radio station carried on the unmanned aerial vehicle communicates with the ground wireless data transmission radio station in a wireless mode. According to the present invention, different road surface information can be collected and subjected to image processing respectively, the corresponding models are established, so that the roadsurface can be dynamically detected in real time.

The invention discloses an immunofluorescence kit for detecting CEA expression of peripheral blood circulating tumor cells of a gastric cancer patient and a detection method. Circulating tumor cells (CTC) in peripheral blood of an advanced gastric cancer patient are separated by using a membrane filtration device, and the CEA expression condition on CTC is further detected by using an immunofluorescence technology. According to the detection method provided by the invention, the CEA expression condition of a patient suffering from advanced or recurrent gastric cancer can be detected without obtaining a tissue specimen through needle biopsy. According to the method provided by the invention, the circulating tumor cells are well separated, the interference of blood cells can be avoided, a false positive result caused by an edge effect possibly generated in a dyeing process can be avoided, the stability is good, the loss of the cells is reduced, and the detection accuracy is improved. Themarker CEA shows good specificity and sensitivity in use, and the application of the marker CEA in gastric cancer is improved.

The invention relates to a method for recognizing peripheral blood circulating tumor cells of a tumor patient based on isolation by size of epithelial tumor cells (ISET), in particular for recognizingcirculating tumor cells which are captured by a physical method and are difficult to recognize by cell morphology, and belongs to the technical field of molecular biology. Whether CD45 and CD31 on suspected CTCs are expressed or not is detected by adopting a cell immunochemical method so as to exclude leukocytes and endothelial cells. Circulating tumor cells in peripheral blood of various cancerpatients can be relatively accurately recognized, and the technology belongs to fields of minimally invasive technologies and can be used for real-time dynamic detection. According to the method provided by the invention, CTC can be detected more accurately, the false positive rate of CTC detection is reduced, the specificity is improved, and the method has important clinical guidance significancein evaluating the illness state and the curative effect by clinically applying CTC detection.

The invention provides a kit and a detection method for detecting protein expression of peripheral blood circulating tumor cells AR-V7 of a prostate cancer patient and. The kit comprises a diluent, adecolorizing solution, a staining solution A, a staining solution B, a mouse anti-human AR-V7 primary antibody, a horse radish peroxidase labeled goat anti-mouse secondary antibody, 0.1% Triton X-100,0.3% H2O2 and a reagent A, according to the detection method, a membrane filtration device is used for separation to obtain peripheral blood circulating tumor cells CTC, and an immunohistochemical technology is used for detecting the AR-V7 expression condition of the peripheral blood CTC. By utilizing the detection method provided by the invention, the AR-V7 expression condition of a patient withadvanced or recurrent hepatocellular carcinoma can be detected without acquiring a tissue specimen through needle biopsy, and real-time dynamic detection can be realized by utilizing a minimally invasive technology; by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by theedge effect possibly generated in the dyeing process is avoided, the stability is good, the cell loss is reduced, and the detection accuracy is improved.

The invention provides an immunofluorescence kit for E-Cadherin gene expression of peripheral blood circulating tumor cells of a colorectal cancer patient and a detection method, and belongs to the field of molecular biology. Particularly, the kit mainly comprises a primary antibody suspension composed of goat serum, mouse anti-CK, rat anti-CD45 and rabbit anti-E-Cadherin, and a secondary antibodysuspension composed of a fluorescently-labeled goat anti-mouse, a fluorescently-labeled goat anti-rat and a fluorescently-labeled goat anti-rabbit. The detection method mainly comprises the followingsteps of: collecting peripheral blood, treating the peripheral blood, filtering and enriching the circulating tumor cells, and detecting the E-Cadherin gene expression condition of the circulating tumor cells by an immunofluorescence method.

The invention provides a kit and method for detecting C-MET gene mutation of a hepatocellular carcinoma patient through peripheral blood circulating tumor cells. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a rabbit anti-human C-MET primary antibody, a biotin labeled mouse anti-rabbit secondary antibody, 0.1% Triton X-100, 0.3% H2O2 and a reagent A. The detection method comprises the following steps: separating and obtaining peripheral blood circulating tumor cells CTC by using a membrane filtration device, and detecting the C-MET expression condition of the peripheral blood CTC by using an immunohistochemical technology. By utilizing the detection method provided by the invention, the C-MET expression condition of the patient with advanced or recurrent hepatocellular carcinoma needle biopsy can be obtained needless of obtaining specimen by puncture, and real-time dynamic detection can be realized by utilizing a minimally invasivetechnology; by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by the edgeeffect possibly generated in the dyeing process is avoided, the stability is high, the cell loss is reduced, and the detection accuracy is improved.