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40results about How to "Real-time dynamic detection" patented technology
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Dynamic measurement method of geometric parameters of overhead contact system
InactiveCN104748685ADoes not affect normal operationSolve the inconvenience of point measurement of industrial hand-held equipmentUsing optical meansSecure stateLight beam
The invention relates to the field of rail traffic detection, in particular to a dynamic measurement method of geometric parameters of an overhead contact system. A trolley is disposed on a steel rail and can move along the same. Linear array cameras and a linear laser are disposed on a body of the trolley; a light-emitting face of the linear laser is aligned to the overhead contact system to be measured; the linear array cameras are disposed on two sides of the linear laser; the emitting face of each linear array camera is aligned to face the overhead contact system; after the trolley is pushed and the linear array cameras are started, the linear array cameras are enabled to continuously shoot the overhead contact system to acquire image data along with movement of the trolley on the steel rail; images of the overhead contact system are analyzed and processed according to the technical principle of trigonometric stereo measurement. The method allows geometric parameters and wear of the overhead contact system and the safety state of hangers of the overhead contact system can be dynamically detected continuously and precisely in real time.
Owner:INST OF SCI & TECH SHANGHAI RAILWAYBUREAU
Local electrochemical imaging test system based on surface plasma resonance
ActiveCN103675053AReal-time dynamic detectionLarge dynamic detection rangeScattering properties measurementsMaterial electrochemical variablesElectrochemical responseData acquisition
The invention discloses a local electrochemical imaging test system based on surface plasma resonance. The local electrochemical imaging test system comprises an optical system, a mechanical system, a data acquiring system, a circulation system, an electrochemical system and a computer control system, wherein the optical system comprises an SPR (surface plasma resonance) chip, an optical prism and a light source; the light source emits an incident laser and irradiates to the optical prism; the circulation system comprises a micro-fluidic test tank, wherein a to-be-tested article flows through the upper surface of the SPR chip via the micro-fluidic test tank to cause the change of a surface plasma resonance absorption peak; the electrochemical system provides electrical stimulation to the to-be-tested article to carry out the electrochemical reaction on the to-be-tested article and acquires corresponding electrochemical signals; the data acquiring system acquires the change of the surface plasma resonance absorption peak; the mechanical system is used for adjusting the angle of the incident laser emitted from the light source; and the computer system obtains corresponding information of the to-be-tested article according to the electrochemical signal and the change of the surface plasma resonance absorption peak.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI
Rare earth upconversion nano fluorescent probe and preparation and application thereof
InactiveCN103865537ASmall and uniform particle sizeEasy to prepareFluorescence/phosphorescenceLuminescent compositionsNear infrared laserRare earth
The invention relates to a rare earth upconversion nano fluorescent probe and preparation and an application thereof. Heterogeneous functional fluorescent nanoparticles are formed from upconversion fluorescent nanoparticles and Au nanoparticles; the molar ratio of the upconversion fluorescent nanoparticles NaYF4:Yb<3+> / Er<3+> to Au is 5:1, 10:1, 15:1 or 20:1, and stirring is carried out for 2.5 h at the temperature of 140 DEG C; compared with the upconversion fluorescent nanoparticles, the obtained particles have red fluorescence enhanced and green fluorescence decreased. The rare earth upconversion fluorescent nanoparticles are good in photochemical stability and low in toxicity; and moreover, a near-infrared laser is used as an excitation light source, the rare earth upconversion fluorescent nanoparticles have strong optical penetration ability, have no background fluorescence, and have potential application value in the fields of biological fluorescence imaging and CT developer.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Online detecting device and online detecting method for synchronous belt type corn kernel breaking rate
ActiveCN109085170AImprove detection efficiencyHigh precisionInvestigation of vegetal materialInvestigating moving fluids/granular solidsInformation processingData information
The invention relates to an online detecting device and online detecting method for synchronous belt type corn kernel breaking rate. The device comprises: a detecting box, a blanking unit, a mechanismexecuting unit and a detecting unit, wherein the blanking unit comprises a blanking slot and a blanking chute, the mechanism executing unit is in a synchronous belt transmission structure, and the detecting unit comprises a CCD industrial camera, a processing chip, and an in-vehicle control and display terminal. Each of the units cooperates with each other to transmit data in real time to ensurethe smooth online detection of the corn kernel breaking rate. The method comprises the following steps: the CCD industrial camera collects corn kernel images and outputs the images to the processing chip for processing to identify broken corn kernels, and the in-vehicle control and display terminal calculates a corn kernel breaking rate. The device provided by the invention is small in size, simple and reliable in structure, convenient to carry, easy to be installed with the combine harvester grain box, high in clarity of images acquired by the CCD industrial camera, simple and efficient in algorithm, and fast in image data information processing speed, so that advantages of high detection efficiency, strong reliability and high precision are obtained.
Owner:SHENYANG INST OF AUTOMATION - CHINESE ACAD OF SCI
High flux multiparameter imaging surface plasma resonance test instrument
ActiveCN101046446AWide range of resonance angle modulationHigh resolutionScattering properties measurementsElectricityHigh flux
The present invention is one kind of high flux multiparameter image surface plasma resonance test instrument, and relates to detection technology. The test instrument in synchronous scanning structure has a triangular optical prism, an array surface plasma resonance chip, a micro measuring pond with at least one flow channel, an automatic sampling system communicated with the micro measuring pond, an optical system, a mechanical scanning system, a data acquiring system, an embedded microprocessor, and a computer with information acquiring and processing software and system operating software for controlling the test instrument. It operates in one of three operation modes to complete quantitative high flux multiparameter detection.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI
Method and device for measuring dynamic viscosity based on light suspended particles
ActiveCN108645751AHigh measurement accuracyQuick responseIndirect flow property measurementPhysicsOptical power
Owner:ZHEJIANG UNIV
Detecting system capable of accurately acquiring ECL (electrochemiluminescence) spectral information
InactiveCN105717100ARealize collaborative operationReduce background noise signalChemiluminescene/bioluminescenceMaterial electrochemical variablesElectrochemiluminescenceOptoelectronics
The invention relates to a detecting system capable of accurately acquiring ECL (electrochemiluminescence) spectral information. The detecting system comprises an electrochemistry equipment unit, an ECL radiation generation device unit, a spectrum detection device unit and a data processing and control unit, wherein the ECL radiation generation device unit comprises an ECL cell as well as a working electrode, a reference electrode and a counter electrode which are connected with the electrochemistry equipment unit; the spectrum detection device unit comprises a collection objective lens and a CCD grating spectrometer; the ECL radiation generation device unit and the collection objective lens are arranged in a cassette, a luminous surface of the working electrode faces the collection objective lens, and the focus of the collection objective lens coincides with an entrance slit of the CCD grating spectrometer; the cassette is directly connected with a fixing plate of the entrance slit, and a detecting system light path is isolated from the outside; the CCD grating spectrometer and the electrochemistry equipment unit are triggered through TTL (transistor transistor logic) signals to realize synchronous operation, and the acquired electrochemical signals and ECL spectrum signals are synchronously input into the data processing and control unit.
Owner:SHANDONG UNIV
Bus-based seawater desalination control system
InactiveCN103130292AImprove the status quo of lack of control functionsImprove utilization efficiencyGeneral water supply conservationSeawater treatmentFrequency changerProgrammable logic controller
The invention discloses a bus-based seawater desalination control system. In the prior art, the heat collection efficiency is low, the water production rate is low, and the system is completely in a spontaneous extensive state, so that the requirements for practical application can not be met. The seawater desalination control system comprises a light gathering and heat collection part, a heat accumulation and heat exchange part, a seawater desalination part, an analog input module, a remote I / O (input / output) module, a PROFIBUS bus, a frequency converter, a PLC (programmable logic controller), and an upper computer, wherein a flow transmitter, a pressure transmitter, a temperature transmitter and a liquid level transmitter acquires signals and input the signals into the analog input module; the analog input module is connected with the remote I / O module; the remote I / O module is connected with the PLC through the bus; the PLC is connected with the upper computer through RS485; and the PLC simultaneously controls the frequency converter via the bus through the remote I / O module. The invention is simple in structure, stable in system, convenient to maintain, mature in technology, favorable in control effect and low in cost.
Owner:HANGZHOU DIANZI UNIV
Device based on micro-nano fluid control reflection interference spectral imaging system and realization method
InactiveCN106053392ARealize dynamic detectionHigh degree of integrationPhase-affecting property measurementsSpectrum generation using multiple reflectionMicro nanoFluid control
The invention discloses a device based on a micro-nano fluid control reflection interference spectral imaging system and a realization method. The device comprises a micro fluid pump, a micro fluid control sample cell system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection light source, and a computer. The C-port optical path switcher is connected to the positioning light source, the CCD sensor, and the spectrometer through optical fibers; the CCD sensor and the spectrometer are both connected to the computer, and the computer software collects detection data and analyzes the results. According to the system, the object lens of the microscope is taken as the optical probe; compared with the conventional method that takes a detector as the probe, the system has the advantages that a fixing device for fixing a probe is not needed, the optical path integration degree of the system is improved; moreover, an imaging and positioning system is used to obtain the specific position of a sample, and thus the detection precision is improved. The morphology feature imaging of samples and reflection interference spectrum collection can be switched in real time, and thus the in-situ dynamic realtime detection of an analysis sample can be realized.
Owner:NORTHWEST UNIV(CN)
Scanning local enhanced biochemical sensing device
InactiveCN108106994AHigh precisionImprove throughputColor/spectral properties measurementsBeam splitterLighting spectrum
The invention discloses a scanning local enhanced biochemical sensing device. Light beams emitted by a light source system are vertically focused in a multi-channel microfluidic sensing unit of a two-dimensional scan temperature control system; the two-dimensional scan temperature control system controls an array sensing unit to allow the focused light beams to pass in sequence and reciprocate soas to scan circularly, and at the same time, a spectrometer acquires a corresponding light spectrum in real time; an automatic sample feeding and rinsing system injects a tested sample to the multi-channel microfluidic sensing unit; a light beam transmitting through the sensing unit is divided into two light beams through a beam splitter of a microscopic imaging system, transmitted light enters aCCD (Charge Coupled Device) detector in the microscopic imaging system so as to image and a signal is transmitted to a computer and an upper computer analysis system to processed and analyzed; reflected light enters the spectrometer, then spectrum data collected by the spectrometer is transmitted to the computer and the upper computer analysis system to be processed and analyzed, and the computerand the upper computer analysis system display multi-channel detection conditions in real time. Through adoption of the scanning local enhanced biochemical sensing device, high-throughput, multi-channel parallel detection and real-time dynamic detection are realized.
Owner:INST OF OPTICS & ELECTRONICS - CHINESE ACAD OF SCI
Method, system and device for detecting hidden diseases of ballastless track
ActiveCN113281401AUndisturbedIntelligentCharacter and pattern recognitionNeural architecturesImaging processingTrackway
The invention discloses a method for detecting hidden diseases of a ballastless track of a railway. The method comprises the following steps that: a relation set of ballastless tracks of preset disease types and corresponding preset characteristic parameter points is established, wherein the ballastless tracks of the preset disease types are disease tracks containing cavities of different shapes, and the preset characteristic parameter points are characteristic expressions of geomagnetic signals; geomagnetic field signals of a to-be-detected ballastless track are collected through a signal collecting device, and characteristic extraction is performed to obtain to-be-detected characteristic parameter points; and the relation between the to-be-detected characteristic parameter points and the preset characteristic parameter points in the relation set by adopting a preset identification model so as to judge the disease type and the corresponding position of the ballastless track of the railway; the hidden disease maintenance level of the ballastless track is diagnosed on the basis of the structural characteristics and maintenance regulations of the detected track; and imaging processing is performed according to the corresponding relation set of the to-be-detected characteristic parameter points and the ballastless track disease types, and disease characteristics are labeled. The invention further provides a corresponding system and device.
Owner:TONGJI UNIV
Kit for detecting mutation expression of BRAF gene V600E of colorectal cancer patient through peripheral blood circulating tumor cells
InactiveCN111521801AReal-time dynamic detectionReduce lossesCell dissociation methodsBiological material analysisGenes mutationOncology
The invention provides a kit for detecting mutation expression of a BRAF gene V600E of a colorectal cancer patient through peripheral blood circulating tumor cells, and belongs to the field of molecular biology, and concretely the kit comprises a primary antibody suspension composed of goat serum, mouse anti-CK, mouse anti-CD45 and rabbit anti-BRAF gene V600E mutation, and a secondary antibody suspension composed of a fluorescently-labeled goat anti-mouse, a fluorescently-labeled goat anti-mouse and a fluorescently-labeled goat anti-rabbit. The detection method mainly comprises the following steps: collecting peripheral blood, treating the peripheral blood, filtering and enriching the circulating tumor cells, and detecting the mutation expression condition of the BRAF gene V600E of the circulating tumor cells by an immunofluorescence method.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Immunofluorescence kit for detecting PD-L1 gene expression of esophageal squamous cell carcinoma patient through peripheral blood circulating tumor cells
InactiveCN111521799AReal-time dynamic detectionReduce lossesBiological material analysisTumor/cancer cellsOncologyTumor cells
The invention provides an immunofluorescence kit for detecting PD-L1 gene expression of an esophageal squamous cell carcinoma patient through peripheral blood circulating tumor cells, and belongs to the field of molecular biology, and particularly the kit comprises a primary antibody suspension composed of goat serum, mouse anti-CK, mouse anti-CD45 and rabbit anti-PD-L1 genes, and a secondary antibody suspension composed of a fluorescently-labeled goat anti-mouse, a fluorescently-labeled goat anti-mouse and a fluorescently-labeled goat anti-rabbit. The detection method mainly comprises the following steps: collecting peripheral blood, treating the peripheral blood, filtering and enriching the circulating tumor cells, and detecting the PD-L1 gene expression condition of the circulating tumor cells by an immunofluorescence method.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Kit and method for screening prostate cancer
PendingCN110923328AThe result is accurateGuaranteed accuracyMicrobiological testing/measurementDNA/RNA fragmentationCancer researchProstate cancer
The invention relates to the technical field of biology, provides a kit and a method for screening prostate cancer and aims at solving the risk assessment problem of the prostate cancer. The kit for screening the prostate cancer, provided by the invention, comprises an ERG primer and a probe thereof, an SPDF primer and a probe thereof, an MAX primer and a probe thereof, an IL32 primer and a probethereof as well as a PDGFA primer and a probe thereof; and an internal control is an SPK primer and a probe thereof. By adopting the kit, a specimen is collected by adopting a non-invasive means, real-time dynamic detection can be performed, and the detection accuracy is high.
Owner:广州中鑫基因医学科技有限公司
Video analysis method and system for illegal behaviors of safe operation
PendingCN114067244AMany types of detectionReal-time dynamic detectionCharacter and pattern recognitionIndividual entry/exit registersSoftware systemImage manipulation
The invention discloses a video analysis system and method for illegal behaviors of safe operation, and belongs to the field of image processing. The system comprises a hardware system and a software system. The software system comprises a video analysis subsystem which runs on the hardware platform of the video analysis workstation; and the video analysis subsystem comprises a real-time video reading module, a video analysis module, an illegal behavior picture storage module, a network alarm module and a log recording module. The video analysis subsystem can analyze videos collected by a cigarette factory roller operation camera, and automatic detection of four typical illegal behaviors that employees do not wear safety helmets and anti-skid shoes, roller switches are not padlocked and signboards are not hung is achieved; a video analysis method for detecting the four illegal behaviors is designed on the basis of a traditional visual detection algorithm such as safety helmet detection; the test result verifies the correctness of the system function; and according to the method, the capability of preventing the safety risk of roller operation is improved.
Owner:河北拓英智能科技有限公司 +1
Multiple channel surface plasma resonant image sensor based on-chip PCR
InactiveCN100487137CEasy to detectEasy to measureMicrobiological testing/measurementPolarisation-affecting propertiesPrismPlasma resonance
Owner:NANKAI UNIV
Kit for detecting PD-L1 gene mutation of peripheral blood circulating tumor cells of prostate cancer patient and detection method
InactiveCN111596056AReal-time dynamic detectionReduce lossesCell dissociation methodsBiological material analysisStainingBiology
The invention provides a kit for detecting PD-L1 gene mutation of peripheral blood circulating tumor cells of a prostate cancer patient and a detection method. The kit comprises a diluent, a destaining , a staining solution A, a staining solution B, an anti-human PD-L1 primary antibody, an enzyme-labeled goat anti-human secondary antibody, 0.1% of Triton X-100, 0.3% of H2O2 and a reagent A. The detection method comprises the following steps: separating and acquiring peripheral blood circulating tumor cells (CTC) by using a membrane filtration device, and detecting the PD-L1 expression condition of the peripheral blood CTCs by using an immunohistochemical technology. By utilizing the detection method provided by the invention, the PD-L1 expression condition of a patient with advanced or recurrent hepatocellular carcinoma can be detected without acquiring a tissue specimen through needle biopsy, and real-time dynamic detection can be realized by utilizing a minimally invasive technology;by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by the edge effect possibly generated in the dyeing process is avoided, the stability is good, the cell loss is reduced, and the detection accuracy is improved.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
In-acupoint in-vivo dynamic electrical-characteristic detecting device and detecting method
InactiveCN103815904ATest results are stableGood repeatabilityDiagnostic recording/measuringSensorsTime dynamicsElectricity
The invention discloses an in-acupoint in-vivo dynamic electrical-characteristic detecting device. The detecting device comprises an upper computer and a lower computer. The upper computer is connected with the lower computer through a serial communication module. Two electrode wires of the detecting device are led out to be connected with handles of acupuncture needles. When the detecting device is started to detect acupoint resistance, the lower computer transmits collected data to the upper computer, and real-time dynamic oscillogram display is realized. The detecting device has the advantages that in-vivo, real-time and dynamic detection of the acupoint resistance is realized, and detection results are stable by constant-flow-source detection.
Owner:SHANGHAI UNIV OF T C M
An unmanned aerial vehicle-mounted pavement detection system and detection method
ActiveCN109902668AReal-time dynamic detectionCharacter and pattern recognitionClosed circuit television systemsFlight heightImaging processing
Owner:SHANDONG UNIV
High flux multiparameter imaging surface plasma resonance test instrument
ActiveCN100535641CWide range of resonance angle modulationHigh resolutionScattering properties measurementsElectricityHigh flux
The present invention is one kind of high flux multiparameter image surface plasma resonance test instrument, and relates to detection technology. The test instrument in synchronous scanning structure has a triangular optical prism, an array surface plasma resonance chip, a micro measuring pond with at least one flow channel, an automatic sampling system communicated with the micro measuring pond, an optical system, a mechanical scanning system, a data acquiring system, an embedded microprocessor, and a computer with information acquiring and processing software and system operating software for controlling the test instrument. It operates in one of three operation modes to complete quantitative high flux multiparameter detection.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI
Bipolar Acupuncture Needles
ActiveCN103816046BAvoid Traditional InfluencersSynchronous needlingAcupunctureAcupuncture needlesNeedle penetration
The invention discloses a double-electrode acupuncture needle which comprises a needle body and a needle handle. An insulating layer is wrapped on the needle body being 0.3cm away from a needle tip, an electroconductive layer is coated on the insulating layer, an electrode leading-out line is bonded on the electroconductive layer and then covered by an insulating sleeve to serve as a first electrode, the needle handle serves as a second electrode, and the two electrodes are combined on an acupuncture needle. The double-electrode acupuncture needle has the advantages that in-vivo, realtime and dynamic detection of acupoint electrical impedance is realized, conventional influencing factors in the process of detection are avoided, and synchronousness of acupuncture and detection is realized.
Owner:SHANGHAI UNIV OF T C M
An unmanned aerial vehicle-mounted pavement detection system and detection method
InactiveCN109447047AReal-time dynamic detectionCharacter and pattern recognitionClosed circuit television systemsFlight heightImaging processing
The invention discloses an unmanned aerial vehicle-mounted pavement detection system and detection method, and the system comprises an unmanned aerial vehicle which is provided with a GPS system and an unmanned aerial vehicle flight height control system. A camera is carried on the unmanned aerial vehicle and is connected with an image sensor; the output end of the image sensor is connected to theinput end of the signal amplification circuit, and the output end of the signal amplification circuit is connected to the input end of the signal conversion circuit; the output end of the signal conversion circuit is connected to a wireless data transmission radio station; the input end of the signal amplification circuit is connected with the output end of the signal detection and processing circuit, and the input end of the signal detection and processing circuit is connected with the controller. The ground wireless data transmission radio station is connected with the ground server throughthe level adapter plate, and the wireless data transmission radio station carried on the unmanned aerial vehicle communicates with the ground wireless data transmission radio station in a wireless mode. According to the present invention, different road surface information can be collected and subjected to image processing respectively, the corresponding models are established, so that the roadsurface can be dynamically detected in real time.
Owner:SHANDONG UNIV
Immunofluorescence kit for detecting CEA expression of peripheral blood circulating tumor cells of gastric cancer patient and detection method
InactiveCN111650376AAvoid interferenceAvoid edge effectsBiological testingStationary filtering element filtersCirculating tumor cellNeedle biopsy
The invention discloses an immunofluorescence kit for detecting CEA expression of peripheral blood circulating tumor cells of a gastric cancer patient and a detection method. Circulating tumor cells (CTC) in peripheral blood of an advanced gastric cancer patient are separated by using a membrane filtration device, and the CEA expression condition on CTC is further detected by using an immunofluorescence technology. According to the detection method provided by the invention, the CEA expression condition of a patient suffering from advanced or recurrent gastric cancer can be detected without obtaining a tissue specimen through needle biopsy. According to the method provided by the invention, the circulating tumor cells are well separated, the interference of blood cells can be avoided, a false positive result caused by an edge effect possibly generated in a dyeing process can be avoided, the stability is good, the loss of the cells is reduced, and the detection accuracy is improved. Themarker CEA shows good specificity and sensitivity in use, and the application of the marker CEA in gastric cancer is improved.
Owner:山东凯歌智能机器有限公司
Vibration test platform for deep hole processing
ActiveCN109342000AExpand the adjustable range of parametersImprove functionalityVibration testingEngineeringTest platform
The invention discloses a vibration test platform for deep hole processing, which relates to the field of deep hole processing technology test. The vibration test platform includes a machine tool. A vibration excitation module, an intermediate module and a feed module are installed in turn from left to right on the machine tool. Through the cooperation of the vibration excitation module, the intermediate module and the feed module, any one, two, three or four tests among torsional vibration loading test, axial vibration loading test, radial vibration test and double radial vibration test can be carried out under a combined condition of different rotation states of a drill pipe and a work piece. Therefore, the adjustable range of parameters of the test platform is extended, and the functionality of the test platform is increased.
Owner:HENAN POLYTECHNIC UNIV
Method for recognizing peripheral blood circulating tumor cells of tumor patient based on isolation by size of epithelial tumor cells
PendingCN111610334AReduce false positive rateHigh specificityBioreactor/fermenter combinationsCell dissociation methodsCirculating cancer cellCirculating tumor cell
The invention relates to a method for recognizing peripheral blood circulating tumor cells of a tumor patient based on isolation by size of epithelial tumor cells (ISET), in particular for recognizingcirculating tumor cells which are captured by a physical method and are difficult to recognize by cell morphology, and belongs to the technical field of molecular biology. Whether CD45 and CD31 on suspected CTCs are expressed or not is detected by adopting a cell immunochemical method so as to exclude leukocytes and endothelial cells. Circulating tumor cells in peripheral blood of various cancerpatients can be relatively accurately recognized, and the technology belongs to fields of minimally invasive technologies and can be used for real-time dynamic detection. According to the method provided by the invention, CTC can be detected more accurately, the false positive rate of CTC detection is reduced, the specificity is improved, and the method has important clinical guidance significancein evaluating the illness state and the curative effect by clinically applying CTC detection.
Owner:SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
Kit and detection method for detecting protein expression of peripheral blood circulating tumor cell AR-V7 of prostate cancer patient
PendingCN111751531AReal-time dynamic detectionReduce lossesCell dissociation methodsBiological material analysisStainingHorse radish peroxidase
The invention provides a kit and a detection method for detecting protein expression of peripheral blood circulating tumor cells AR-V7 of a prostate cancer patient and. The kit comprises a diluent, adecolorizing solution, a staining solution A, a staining solution B, a mouse anti-human AR-V7 primary antibody, a horse radish peroxidase labeled goat anti-mouse secondary antibody, 0.1% Triton X-100,0.3% H2O2 and a reagent A, according to the detection method, a membrane filtration device is used for separation to obtain peripheral blood circulating tumor cells CTC, and an immunohistochemical technology is used for detecting the AR-V7 expression condition of the peripheral blood CTC. By utilizing the detection method provided by the invention, the AR-V7 expression condition of a patient withadvanced or recurrent hepatocellular carcinoma can be detected without acquiring a tissue specimen through needle biopsy, and real-time dynamic detection can be realized by utilizing a minimally invasive technology; by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by theedge effect possibly generated in the dyeing process is avoided, the stability is good, the cell loss is reduced, and the detection accuracy is improved.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Immunofluorescence kit for detecting E-Cadherin expression of peripheral blood circulating tumor cells of colorectal cancer patient
InactiveCN111965363AReal-time dynamic detectionReduce lossesBiological material analysisBiological testingOncologyTumor cells
The invention provides an immunofluorescence kit for E-Cadherin gene expression of peripheral blood circulating tumor cells of a colorectal cancer patient and a detection method, and belongs to the field of molecular biology. Particularly, the kit mainly comprises a primary antibody suspension composed of goat serum, mouse anti-CK, rat anti-CD45 and rabbit anti-E-Cadherin, and a secondary antibodysuspension composed of a fluorescently-labeled goat anti-mouse, a fluorescently-labeled goat anti-rat and a fluorescently-labeled goat anti-rabbit. The detection method mainly comprises the followingsteps of: collecting peripheral blood, treating the peripheral blood, filtering and enriching the circulating tumor cells, and detecting the E-Cadherin gene expression condition of the circulating tumor cells by an immunofluorescence method.
Owner:山东凯歌智能机器有限公司
An immunofluorescence kit and detection method for detecting PD-L1 expression in peripheral blood circulating tumor cells of patients with gastric cancer
ActiveCN111562375BReal-time dynamic detectionReduce lossesCell dissociation methodsBiological material analysisStainingOncology
The invention discloses an immunofluorescence kit and a detection method for detecting PD‑L1 expression of peripheral blood circulating tumor cells of patients with gastric cancer. A membrane filtration device is used to separate and obtain circulating tumor cells (CTCs) in the peripheral blood of patients with advanced gastric cancer, and further use immunofluorescence The technology detects the expression of PD-L1 on CTC. The detection method provided by the present invention can detect the expression of PD‑L1 in patients with advanced or recurrent gastric cancer without obtaining tissue samples by needle biopsy. The method provided by the invention has good separation of circulating tumor cells, can avoid the interference of blood cells, can avoid false positive results caused by possible edge effects in the staining process, has good stability, reduces cell loss, and improves detection accuracy. The use of the marker PD‑L1 showed good specificity and sensitivity, which improved its application in gastric cancer.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
Device and implementation method of reflection interference spectroscopy imaging system based on micro-nanofluidics
InactiveCN106053392BRealize dynamic detectionHigh degree of integrationPhase-affecting property measurementsSpectrum generation using multiple reflectionEngineeringMicroscope objective
The invention discloses a device and an implementation method based on a micro-nanofluidic reflection interference spectroscopy imaging system, including a microfluidic pump, a microfluidic sample pool system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection The light source, the computer, and the C-port optical path switcher are respectively connected to the positioning light source, CCD sensor and spectrometer through optical fibers. Both the CCD sensor and the spectrometer are connected to the computer, and the computer software collects the detection data and analyzes the results. The present invention uses the objective lens of the microscope as the optical probe, compared with the method using the detector as the probe in the prior art, it does not need to be provided with a fixing device for fixing the probe, which improves the optical path integration degree of the system; it is determined by the imaging positioning system The specific position of the sample improves the detection accuracy. The invention realizes the real-time switching between the imaging of the shape feature of the sample and the acquisition of the reflection interference spectrum, and realizes the in-situ dynamic real-time detection of the analyzed sample.
Owner:NORTHWEST UNIV
Method for detecting C-MET gene mutation of hepatocellular carcinoma patient through peripheral blood circulating tumor cells
InactiveCN111638340AReal-time dynamic detectionReduce lossesBiological material analysisStationary filtering element filtersGenes mutationStaining
The invention provides a kit and method for detecting C-MET gene mutation of a hepatocellular carcinoma patient through peripheral blood circulating tumor cells. The kit comprises a diluent, a decolorizing solution, a staining solution A, a staining solution B, a rabbit anti-human C-MET primary antibody, a biotin labeled mouse anti-rabbit secondary antibody, 0.1% Triton X-100, 0.3% H2O2 and a reagent A. The detection method comprises the following steps: separating and obtaining peripheral blood circulating tumor cells CTC by using a membrane filtration device, and detecting the C-MET expression condition of the peripheral blood CTC by using an immunohistochemical technology. By utilizing the detection method provided by the invention, the C-MET expression condition of the patient with advanced or recurrent hepatocellular carcinoma needle biopsy can be obtained needless of obtaining specimen by puncture, and real-time dynamic detection can be realized by utilizing a minimally invasivetechnology; by adopting the separation device and the diluent, the circulating tumor cells are well separated, the interference of blood cells is avoided, the false positive result caused by the edgeeffect possibly generated in the dyeing process is avoided, the stability is high, the cell loss is reduced, and the detection accuracy is improved.
Owner:山东凯歌智能机器有限公司
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