40results about How to "Real-time dynamic detection" patented technology

The invention relates to a biosensor based on the surface plasma resonance principle, in particular to use the biology PCR technology combined with it. Which including the following steps: a) combining the SPR sample pond with the PCR reflecting pond and formatting the sensor reflecting pond; (b) the top of the sensor body is a rectangular reflecting sensor pond made up of the silicon chip, the lower is a cylinder prism, the two sides of the sensor body are set with the incident path, exit path and the computer plate connected with the end of the exit path;(c) the homogeneous light incidences, using the single color surface CCD to collect SPR picture and analyze through it, measuring the resonance and the change of it, which means the response of each channel. The invention has for characters: (a) measuring several samples once (b) measuring different characters of a sample once; (c) setting the consulting channel in the multi-channel and avoiding the effect of non-specific; (d) measuring the DNA molecule of low concentration.

The invention discloses an unmanned aerial vehicle-mounted pavement detection system and detection method, and the system comprises an unmanned aerial vehicle which is provided with a GPS system and an unmanned aerial vehicle flight height control system. A camera is carried on the unmanned aerial vehicle and is connected with an image sensor; The output end of the image sensor is connected to theinput end of the signal amplification circuit, and the output end of the signal amplification circuit is connected to the input end of the signal conversion circuit; The output end of the signal conversion circuit is connected to a wireless data transmission radio station; The input end of the signal amplification circuit is connected with the output end of the signal detection and processing circuit, and the input end of the signal detection and processing circuit is connected with the controller. The ground wireless data transmission radio station is connected with the ground server throughthe level adapter plate, and the wireless data transmission radio station carried on the unmanned aerial vehicle communicates with the ground wireless data transmission radio station in a wireless mode. Different road surface information can be collected and subjected to image processing respectively, corresponding models are established, and the road surface can be dynamically detected in real time.

The invention discloses an immunofluorescence kit and a detection method for detecting PD‑L1 expression of peripheral blood circulating tumor cells of patients with gastric cancer. A membrane filtration device is used to separate and obtain circulating tumor cells (CTCs) in the peripheral blood of patients with advanced gastric cancer, and further use immunofluorescence The technology detects the expression of PD-L1 on CTC. The detection method provided by the present invention can detect the expression of PD‑L1 in patients with advanced or recurrent gastric cancer without obtaining tissue samples by needle biopsy. The method provided by the invention has good separation of circulating tumor cells, can avoid the interference of blood cells, can avoid false positive results caused by possible edge effects in the staining process, has good stability, reduces cell loss, and improves detection accuracy. The use of the marker PD‑L1 showed good specificity and sensitivity, which improved its application in gastric cancer.

The invention discloses a device and an implementation method based on a micro-nanofluidic reflection interference spectroscopy imaging system, including a microfluidic pump, a microfluidic sample pool system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection The light source, the computer, and the C-port optical path switcher are respectively connected to the positioning light source, CCD sensor and spectrometer through optical fibers. Both the CCD sensor and the spectrometer are connected to the computer, and the computer software collects the detection data and analyzes the results. The present invention uses the objective lens of the microscope as the optical probe, compared with the method using the detector as the probe in the prior art, it does not need to be provided with a fixing device for fixing the probe, which improves the optical path integration degree of the system; it is determined by the imaging positioning system The specific position of the sample improves the detection accuracy. The invention realizes the real-time switching between the imaging of the shape feature of the sample and the acquisition of the reflection interference spectrum, and realizes the in-situ dynamic real-time detection of the analyzed sample.