Scanning local enhanced biochemical sensing device

Abstract

The invention discloses a scanning local enhanced biochemical sensing device. Light beams emitted by a light source system are vertically focused in a multi-channel microfluidic sensing unit of a two-dimensional scan temperature control system; the two-dimensional scan temperature control system controls an array sensing unit to allow the focused light beams to pass in sequence and reciprocate soas to scan circularly, and at the same time, a spectrometer acquires a corresponding light spectrum in real time; an automatic sample feeding and rinsing system injects a tested sample to the multi-channel microfluidic sensing unit; a light beam transmitting through the sensing unit is divided into two light beams through a beam splitter of a microscopic imaging system, transmitted light enters aCCD (Charge Coupled Device) detector in the microscopic imaging system so as to image and a signal is transmitted to a computer and an upper computer analysis system to processed and analyzed; reflected light enters the spectrometer, then spectrum data collected by the spectrometer is transmitted to the computer and the upper computer analysis system to be processed and analyzed, and the computerand the upper computer analysis system display multi-channel detection conditions in real time. Through adoption of the scanning local enhanced biochemical sensing device, high-throughput, multi-channel parallel detection and real-time dynamic detection are realized.

Description

technical field [0001] The invention belongs to the technical field of biochemical sensing, in particular to a scanning local enhanced biochemical sensing device. Background technique [0002] Biochemical sensing technology is an important branch of sensing technology, which is related to public safety, virus, bacteria detection, clinical medicine, environmental detection and other fields that are closely related to people's lives. Traditional biochemical sensing technology usually requires labeling, and 90% of the workload is used in labeling. Radioactive isotopes, enzymes or fluorescence are mainly used as markers, which have poor safety and stability. At the same time, the system is large in size, low in sensitivity, cumbersome and efficient Low. It cannot meet the needs of rapid, sensitive, specific and high-throughput detection. [0003] In recent years, with the integration of nanotechnology, physical chemistry and life sciences, the excellent electromagnetic propert...

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: in order to overcome the shortcomings of traditional SPR biochemical sensing technology such as low flux, high cost, complex system, and long cycle, a scanning local enhanced biochemical sensing device is provided, which utilizes a two-dimensional scanning method , localized surface plasmon resonance technology and microfluidic technology to achieve high throughput, high sensitivity, label-free, real-time display of results and biochemical sensing detection technology measured at a specific temperature

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