Kit and method for screening prostate cancer

A prostate cancer, kit technology, applied in biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc. Guaranteed accuracy and accurate results

Pending Publication Date: 2020-03-27
广州中鑫基因医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Traditional protein markers, non-tumor-specific, have a significant increase in prostatitis and other diseases, and their increase will also lead to excessive diagnosis and treatment of prostate cancer; molecular markers: such as GSTP1 gene methylation, PCA3 gene excess Expression, etc., the specificity and sensitivity of PCA3 detection are not idea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0074] Example 1

[0075] A kit for screening prostate cancer,

[0076] The ERG primer and its probe are:

[0077] Upstream primer ERG-F GCGTCCTCAGTTAGATCCTTATCAG

[0078] Downstream primer ERG-R CTGGCCACTGCCTGGATT

[0079] Probe:

[0080] Probe1 FAM-CTTGGACCA / ZEN / ACAAGTAGCCGCCTTGC-BHQ1

[0081] The SPDEF primer and its probe are:

[0082] Upstream primer SPDEF-F CCACCTGGACATCTGGAAG

[0083] Downstream primer SPDEF-R CTGGCCACTGCCTGGATT

[0084] Probe: Probe2 VIC-CGGCCTGGA / ZEN / TGAAAGAGCG-BHQ1

[0085] The MAX primer and its probe are:

[0086] Upstream primer MAX-F AAGGGATGAGACTATGTG

[0087] Downstream primer MAX-R GGAGATAATGTTGAGTGC

[0088] Probe: Probe3 CY5-CCTAACATCCACAGAGACATTGCTTG-BHQ2

[0089] The IL32 primer and its probe are:

[0090] Upstream primer IL32-F CTCTCTCGGGTAAGTCTC

[0091] Downstream primer IL32-R CCTGGCACAAATACTCAG

[0092] Probe: Probe4 FAM-TCTCTGTCTGTCTCTGTCTCTGTCT-BHQ1

[0093] The PDGFA primer and its probe are:

[0094] Upstream primer PDGFA -F TTGGGTAAAGATTTGTGATTA

[0095] ...

Example Embodiment

[0101] Example 2

[0102] A kit for screening prostate cancer, comprising: ERG primers and probes, SPDF primers and probes, MAX primers and probes, IL32 primers and probes, PDGFA primers and probes; internal control is SPK primers and probes. The SPK primer and its probe are:

[0103] Upstream primer SPK-F CCTTGAACCATTATGCTG

[0104] Downstream primer SPK-R CCGATACTTCTCACTCTC

[0105] Probe: Probe6 VIC-ACCAATGAAGACCTCCTGACCAA-BHQ1.

Example Embodiment

[0106] Example 3

[0107] A kit for screening prostate cancer, comprising: ERG primers and probes, SPDF primers and probes, MAX primers and probes, IL32 primers and probes, PDGFA primers and probes; internal control is SPK primers and probes.

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Abstract

The invention relates to the technical field of biology, provides a kit and a method for screening prostate cancer and aims at solving the risk assessment problem of the prostate cancer. The kit for screening the prostate cancer, provided by the invention, comprises an ERG primer and a probe thereof, an SPDF primer and a probe thereof, an MAX primer and a probe thereof, an IL32 primer and a probethereof as well as a PDGFA primer and a probe thereof; and an internal control is an SPK primer and a probe thereof. By adopting the kit, a specimen is collected by adopting a non-invasive means, real-time dynamic detection can be performed, and the detection accuracy is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and method for screening prostate cancer. Background technique [0002] Existing prostate cancer screening methods include protein marker detection, DNA marker detection, RNA marker detection, miRNA-based biomarker detection and other methods. [0003] Detection of protein markers: PSA is tissue-specific and only exists in the cytoplasm of human prostate acinar and ductal epithelial cells, and is not expressed in other cells. However, it is not tumor-specific, and prostatitis, benign prostatic hyperplasia, and prostate cancer can all lead to elevated total PSA levels (free PSA plus composite PSA). While PSA is the most commonly used test for prostate cancer, benign prostatic hyperplasia and prostatitis can also produce positive PSA results. The normal value of serum prostate specific antigen (PSA) is generally <4ng / mL, and when prostate cancer occurs, PSA>10ng / mL, which...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/166
Inventor 沈菲李琳琳陈汉奎党琼黄仲曦汪佳宏李高生
Owner 广州中鑫基因医学科技有限公司
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