Method for recognizing peripheral blood circulating tumor cells of tumor patient based on isolation by size of epithelial tumor cells
A technology for peripheral blood circulation and tumor patients, applied in the field of molecular biology, can solve the problem of peripheral blood circulation tumor cells, and achieve the effect of improving specificity, accurate detection of CTC, and reducing false positive rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0044] The present invention will be described below in conjunction with the drawings and embodiments.
[0045] Examples of using this technique to separate, obtain and identify 8 tumor patients (while testing 8 normal samples as negative controls) of circulating tumor cells in the peripheral blood.
[0046] 1. Separation of CTCs in peripheral blood of patients with advanced tumors using membrane filtration devices
[0047] Collect 5ml of fasting blood for 8-12 hours from the median cubital vein, and use 45ml diluent (composition: 1mmol / L EDTA+0.1% BSA+0.1% trehalose+0.2% polyoxyethylene polyoxypropylene ether block copolymer , The basic solution is Tris-HCl buffer) to dilute the peripheral blood, and then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes;
[0048] In a fixed interval, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filtering device is composed of a filter 3, a filter membrane 7, a blood sa...
PUM
Property | Measurement | Unit |
---|---|---|
Diameter | aaaaa | aaaaa |
Diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap