Device and implementation method of reflection interference spectroscopy imaging system based on micro-nanofluidics

Abstract

The invention discloses a device and an implementation method based on a micro-nanofluidic reflection interference spectroscopy imaging system, including a microfluidic pump, a microfluidic sample pool system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection The light source, the computer, and the C-port optical path switcher are respectively connected to the positioning light source, CCD sensor and spectrometer through optical fibers. Both the CCD sensor and the spectrometer are connected to the computer, and the computer software collects the detection data and analyzes the results. The present invention uses the objective lens of the microscope as the optical probe, compared with the method using the detector as the probe in the prior art, it does not need to be provided with a fixing device for fixing the probe, which improves the optical path integration degree of the system; it is determined by the imaging positioning system The specific position of the sample improves the detection accuracy. The invention realizes the real-time switching between the imaging of the shape feature of the sample and the acquisition of the reflection interference spectrum, and realizes the in-situ dynamic real-time detection of the analyzed sample.

Description

technical field [0001] The invention relates to a device and a realization method based on a micro-nano fluidic reflection interference spectrum imaging system. Background technique [0002] A detection method based on micro-nanofluidic reflection interference spectroscopy imaging system is convenient, high-precision, non-contact and non-destructive detection of samples, and can directly observe and collect the surface morphology and reflection interference signals of trace samples. Among them, reflection interference spectroscopy does not require a coherent light source, which makes it simple in structure and stronger in anti-interference ability, and can be well applied to the detection of trace samples. Reflection interference spectroscopy imaging technology has more practical significance and practical value. For example, in the field of microbiology, the reflectance interference spectroscopy imaging system studies the type and quantity of microorganisms by detecting the...

AI Technical Summary

Problems solved by technology

This method uses an optical fiber probe to detect the sample to obtain the interference spectrum signal, which cannot accurately locate the test position and shape characteristics of the tiny sample.

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