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Device and implementation method of reflection interference spectroscopy imaging system based on micro-nanofluidics

A technology of reflection interference and spectral imaging, which is applied in the directions of using multiple reflections to generate spectra, spectrum generation, and measurement of phase-influenced characteristics. The degree of integration and the effect of accurate detection results

Inactive Publication Date: 2019-12-10
NORTHWEST UNIV
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  • Description
  • Claims
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Problems solved by technology

This method uses an optical fiber probe to detect the sample to obtain the interference spectrum signal, which cannot accurately locate the test position and shape characteristics of the tiny sample.

Method used

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  • Device and implementation method of reflection interference spectroscopy imaging system based on micro-nanofluidics
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  • Device and implementation method of reflection interference spectroscopy imaging system based on micro-nanofluidics

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Embodiment

[0052] Embodiments of devices and implementation methods based on micro-nanofluidic reflection interference spectroscopy imaging systems are given below.

[0053] In this embodiment, the device based on the micro-nanofluidic reflection interference spectroscopy imaging system includes a microfluidic pump 1, a microfluidic sample cell system 2, an optical microscope 4, a C-port optical path switcher 5, a CCD sensor 6, a computer 7, and a spectrometer 8. Locating the light source 9 and detecting the light source.

[0054] Microfluidic pump with a flow rate range of 0.004ml / min; the microfluidic sample pool system 2 is a micro-nano structure, the microstructure is 25μmx10μmx15μm, the diameter of the nanostructure is 50nm, and the thickness is 8um; the magnification of the objective lens 3 of the optical microscope 4 is 10 times , numerical aperture 0.70; the detection light source of the optical microscope 4 is a uniform light source halogen lamp, the power is 100W, and the wave ...

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Abstract

The invention discloses a device and an implementation method based on a micro-nanofluidic reflection interference spectroscopy imaging system, including a microfluidic pump, a microfluidic sample pool system, an optical microscope, a C-port optical path switcher, a CCD sensor, a spectrometer, a positioning light source, a detection The light source, the computer, and the C-port optical path switcher are respectively connected to the positioning light source, CCD sensor and spectrometer through optical fibers. Both the CCD sensor and the spectrometer are connected to the computer, and the computer software collects the detection data and analyzes the results. The present invention uses the objective lens of the microscope as the optical probe, compared with the method using the detector as the probe in the prior art, it does not need to be provided with a fixing device for fixing the probe, which improves the optical path integration degree of the system; it is determined by the imaging positioning system The specific position of the sample improves the detection accuracy. The invention realizes the real-time switching between the imaging of the shape feature of the sample and the acquisition of the reflection interference spectrum, and realizes the in-situ dynamic real-time detection of the analyzed sample.

Description

technical field [0001] The invention relates to a device and a realization method based on a micro-nano fluidic reflection interference spectrum imaging system. Background technique [0002] A detection method based on micro-nanofluidic reflection interference spectroscopy imaging system is convenient, high-precision, non-contact and non-destructive detection of samples, and can directly observe and collect the surface morphology and reflection interference signals of trace samples. Among them, reflection interference spectroscopy does not require a coherent light source, which makes it simple in structure and stronger in anti-interference ability, and can be well applied to the detection of trace samples. Reflection interference spectroscopy imaging technology has more practical significance and practical value. For example, in the field of microbiology, the reflectance interference spectroscopy imaging system studies the type and quantity of microorganisms by detecting the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/45G01J3/26
CPCG01J3/26G01N21/45
Inventor 王凯歌武青青孙聃张琛王爽赵伟冯晓强白晋涛
Owner NORTHWEST UNIV
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