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Multiple channel surface plasma resonant image sensor based on-chip PCR

A surface plasmon and image sensor technology, applied in the field of biosensors, can solve the problems of low response time, limited angular resolution, and insufficient measurement accuracy of SPR sensors, and achieve high sensitivity, low purity requirements, and improved detection accuracy and speed. Effect

Inactive Publication Date: 2009-05-13
NANKAI UNIV
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Problems solved by technology

[0012] As mentioned above, these methods suffer from three main drawbacks: low response time and limited angular resolution, SPR sensor measurement accuracy is not high enough

Method used

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  • Multiple channel surface plasma resonant image sensor based on-chip PCR
  • Multiple channel surface plasma resonant image sensor based on-chip PCR
  • Multiple channel surface plasma resonant image sensor based on-chip PCR

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Embodiment 1

[0096] The following experimental steps can be used to determine the surface sensitivity of A, B, and C channels, and at the same time complete the preparation of sensitive membranes. When determining the sensitivity of each channel, use such as figure 2 Shown sample cell (3, silicon substrate, 22, prism placement groove, 23, A channel sample cell, 24, B channel sample cell, 25, C channel sample cell), first to A, B, C three channels At the same time, a mixture of biotinylated thiol derivatives and OH radical thiol diluents (PBS buffer environment) with a molar ratio of 1:9 was introduced, and the reaction was long enough (more than 16 hours) to form a tight coverage on the surface of each channel. The self-assembled monomolecular membrane (SAM) was washed with PBS buffer. Then pass through each channel sequentially with surface plasmon PBS buffer, streptavidin with a sufficiently high concentration (reaction for about 5-20 minutes), and phosphate PBS buffer, and simultaneou...

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Abstract

The invention relates to a biosensor based on the surface plasma resonance principle, in particular to use the biology PCR technology combined with it. Which including the following steps: a) combining the SPR sample pond with the PCR reflecting pond and formatting the sensor reflecting pond; (b) the top of the sensor body is a rectangular reflecting sensor pond made up of the silicon chip, the lower is a cylinder prism, the two sides of the sensor body are set with the incident path, exit path and the computer plate connected with the end of the exit path;(c) the homogeneous light incidences, using the single color surface CCD to collect SPR picture and analyze through it, measuring the resonance and the change of it, which means the response of each channel. The invention has for characters: (a) measuring several samples once (b) measuring different characters of a sample once; (c) setting the consulting channel in the multi-channel and avoiding the effect of non-specific; (d) measuring the DNA molecule of low concentration.

Description

technical field [0001] The invention relates to a biological sensor based on the principle of surface plasmon resonance, in particular to a sensor made by combining biological PCR technology and the principle of plasma resonance, which can detect multiple biological signals in parallel. Background technique [0002] The 21st century is an era of biological development, especially the bioinformatics produced by the fusion of biological science and computer science will be vigorously developed. Sensing technology is one of the main technologies of information science and a means of information acquisition. Using sensing technology to obtain information about biological samples is an important part of the development of biological detection technology. [0003] Among them, the optical method is regarded as the most mature and best biosensing technology due to its non-destructive and high sensitivity characteristics. Since Wood first discovered the surface plasmon resonance (S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/55G01N33/53G01N33/68G01N21/21G01N33/50G01N33/543
Inventor 刘国华牛文成张福海俞梅贾芸芳张维米永巍王宁
Owner NANKAI UNIV
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