Immunofluorescence kit for detecting CEA expression of peripheral blood circulating tumor cells of gastric cancer patient and detection method
A technology for peripheral blood circulation and tumor cells, which is applied in biological testing, separation methods, chemical instruments and methods, etc., can solve problems such as false negatives, false positives in detection methods, and the inability of tissue samples to evaluate the real-time dynamic status of CEA in patients, so as to reduce the number of cells Loss, good stability, avoid the effect of edge effect
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[0049] Example 1
[0050] 1. Use a membrane filtration device to separate and obtain CTC in the peripheral blood of patients with advanced or recurrent gastric cancer who cannot obtain tissue samples to determine whether CTC exists:
[0051] Collect 5ml of fasting blood for 8-12 hours from the median cubital vein, dilute the peripheral blood with 45ml of diluent, and then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes;
[0052] In a fixed interval, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filtering device is composed of a filter 3, a filter membrane 7, a blood sample container 2, a waste liquid tank 5, and an iron stand 1;
[0053] Wet the filter 3 with 10ml PBS, and then add the fixed peripheral blood sample to the blood sample container 2 of the membrane filtration device to make it filter naturally by gravity, and the CTC is trapped on the filter membrane 7;
[0054] The diameter of tumor cells i...
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