An immunofluorescence kit and detection method for detecting PD-L1 expression in peripheral blood circulating tumor cells of patients with gastric cancer

Abstract

The invention discloses an immunofluorescence kit and a detection method for detecting PD‑L1 expression of peripheral blood circulating tumor cells of patients with gastric cancer. A membrane filtration device is used to separate and obtain circulating tumor cells (CTCs) in the peripheral blood of patients with advanced gastric cancer, and further use immunofluorescence The technology detects the expression of PD-L1 on CTC. The detection method provided by the present invention can detect the expression of PD‑L1 in patients with advanced or recurrent gastric cancer without obtaining tissue samples by needle biopsy. The method provided by the invention has good separation of circulating tumor cells, can avoid the interference of blood cells, can avoid false positive results caused by possible edge effects in the staining process, has good stability, reduces cell loss, and improves detection accuracy. The use of the marker PD‑L1 showed good specificity and sensitivity, which improved its application in gastric cancer.

Description

technical field [0001] The invention provides an immunofluorescence kit and detection method for detecting PD-L1 expression in peripheral blood circulating tumor cells of patients with gastric cancer, and belongs to the technical field of molecular biology. Background technique [0002] Gastric cancer (GC) is a highly fatal malignant tumor with the fourth highest incidence in the world (950,000 new cases per year), and the third highest among cancer-related deaths in 2012. Although the diagnosis and treatment methods of gastric cancer have improved in recent years, the 5-year survival rate of gastric cancer patients is less than 30%, and about 50% of gastric cancer patients suffer from tumor recurrence or metastasis after curative resection. Tumor recurrence and metastasis are the main causes of death in gastric cancer patients. [0003] Circulating tumor cells (CTCs) are tumor cells shed from solid tumors and enter the peripheral blood circulation. Since their discovery in...

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Problems solved by technology

[0007] Aiming at the shortcomings in the prior art that the detection of patients with advanced tumors or recurrent gastric cancer cannot obtain tissue samples in real time or repeated punctures, and thus cannot evaluate the real-time dynamic state of the patient's PD-L1, and the existing detection methods are prone to false positives and false negatives, the present invention Provides an immunofluorescence kit and detection method for the expression of PD-L1 in the peripheral blood of patients with gastric cancer, uses a membrane filtration device to separate and obtain circulating tumor cells (CTCs) in the peripheral blood of patients with advanced gastric cancer, and further uses immunofluorescence technology to detect CTCs On the expression of PD-L1

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