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Method for establishing benign prostatic hyperplasia (BPH) canine model with EMT characteristics

A prostate, model technology, applied in animal husbandry and other directions, can solve problems such as lack of systematic research

Inactive Publication Date: 2021-03-02
SHANGHAI INST OF PLANNED PARENTHOOD RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently a lack of systematic research on this

Method used

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  • Method for establishing benign prostatic hyperplasia (BPH) canine model with EMT characteristics
  • Method for establishing benign prostatic hyperplasia (BPH) canine model with EMT characteristics
  • Method for establishing benign prostatic hyperplasia (BPH) canine model with EMT characteristics

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Embodiment 1B

[0059] The establishment method of embodiment 1BPH dog model

[0060] 1. Animals to be modeled

[0061] (1) Species: Dog;

[0062] (2) Department: Beagle;

[0063] (3) Gender and quantity

[0064] ① Spontaneous BPH model: 3 Beagle dogs aged 9-12 months, 5-7 years old and 9-11 years old (representing young, middle-aged and old dogs respectively);

[0065] ② Induced BPH model: 3 Beagle dogs aged 12-15 months (control group and model group).

[0066] (4) Instructions for model animal selection

[0067] ① Among mammals, only canines and some non-human primates can develop spontaneous benign prostatic hyperplasia with age under normal testicular function like humans.

[0068] ②Clinically, benign prostatic hyperplasia mostly occurs in men over 40 years old (equivalent to 6 years old in dogs), and the incidence rate of BPH with clinical symptoms at the age of 50 is 50%, and the incidence rate increases by 10% for every 10 years of age increase. The incidence of BPH in men aged ...

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Abstract

The invention discloses a method for establishing a benign prostatic hyperplasia (BPH) canine model with epithelial-mesenchymal transition (EMT) characteristics, and belongs to the field of animal models and medical evaluation. The BPH canine model refers to a spontaneous BPH canine model and an induced BPH canine model. According to the method, an induced BPH model established in 10 days after estrogen / androgen in a ratio of 1: 100 is given to adult Beagle dogs is adopted, and the induced BPH model has a change trend consistent with that of a spontaneous BPH model in the aspects of prostate index, hormone level, proportion change of epithelium and mesenchyme (similar characteristics to human prostatic hyperplasia) and pathological histological examination, so that the success of the modelis confirmed. Furthermore, marker proteins corresponding to epithelium and mesenchyme are detected by adopting an immunohistochemical technology, and EMT is preliminarily found to be a hyperplasia inducement of fibrosis of interstitial parts of two BPH models. By utilizing the method disclosed by the invention, a stable spontaneous BPH canine model with EMT characteristics and an induced BPH canine model can be established, and the method can be used for screening medicines for treating benign prostatic hyperplasia and evaluating functions of foods or health-care products for improving benignprostatic hyperplasia, and has important application value.

Description

technical field [0001] The invention belongs to the field of animal models and medical evaluation, and in particular relates to a method for establishing a benign prostatic hyperplasia BPH dog model with EMT characteristics. Background technique [0002] Benign prostatic hyperplasia (BPH) is a benign lesion of the prostate. It is common in middle-aged and elderly men over 40 years old. The clinical symptoms are mostly dysuria. The prevalence rate of men shows a clear upward trend with age , about half of men over the age of 50 will develop clinical symptoms. Due to its special physiological location, the prostate gland will squeeze the urethra after hyperplasia, causing a series of clinical manifestations such as urinary obstruction, such as: weak urine stream, frequent urination, urgency, dribbling, incontinent urination, waiting for urination, etc. It has a serious impact on life and life, especially if timely treatment is not paid attention to, it will cause a series of ...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/20A01K2207/30A01K2227/10A01K2267/0331
Inventor 骆永伟孙祖越
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES
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