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Berberis amurensis somatic embryo tissue culture medium and tissue culture method

A technology of cell embryo and Phellodendron amurense, which is applied in the field of tissue culture and plant tissue culture, can solve the problems of low reproduction coefficient and low rooting rate, and achieve the effect of high induction rate and high induction efficiency

Active Publication Date: 2020-09-11
FORESTRY RES INST OF HEILONGJIANG PROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture of Phellodendron amurense has only initially established callus induction and proliferation culture of leaves or stems of Phellodendron amurense, and the rooting rate in rooting culture is extremely low and the reproduction coefficient is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Explant collection

[0025] The age of the forest in Labor Park, Harbin City, Heilongjiang Province is 50 years. From July 1st to July 3rd, the immature zygotic embryos of Phellodendron amurense were in the semi-mature stage.

[0026] (2) explant disinfection

[0027] Move the preliminarily treated seeds into a sterilized ultra-static workbench, stir and sterilize twice with 70% alcohol, 30s each time, rinse with sterile water twice; Rinse with sterile water for 6-8 times; transfer to sterile water for 30 minutes, and dry the filter paper to prepare for inoculation.

[0028] (3) Preparation of medium

[0029] Prepare the following callus induction medium according to the conventional method:

[0030] MS+2,4D 2.0mg / L+6-BA 1.5mg / L+800mg / L hydrolyzed casein.

[0031] (4) Cultivate

[0032] Put the sterilized seeds on the sterile filter paper, cut off part of the seed coat from the end of the cotyledon with a scalpel on the ultra-clean workbench, extrude the embryo...

Embodiment 2

[0035] (1) Explant collection

[0036] The age of labor park in Harbin City, Heilongjiang Province is 50 years. From July 1st to July 3rd, the semi-mature zygotic embryos of Phellodendron amurense have red skin and waxy embryos, but not plump.

[0037] All the other steps are the same as in Example 1.

[0038] When cultured for 15 days after inoculation, the callus induction rate was 42%.

Embodiment 3

[0047]Step (3) uses the following callus induction medium, and the rest of the steps are the same as in Example 1.

[0048] Callus induction medium:

[0049] MS+2,4D 2.0mg / L+6-BA 1.0mg / L+900mg / L hydrolyzed casein.

[0050] When cultured for 15 days after inoculation, the explants on the medium can form callus, the color is white or light yellow, and the vitality is good. The callus induction rate was 36%, and the browning rate was 8%.

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Abstract

The invention relates to the technical field of plant tissue culture, and discloses a berberis amurensis somatic embryo induction medium, a differentiation medium and a corresponding tissue culture method. According to the tissue culture method, a semi-mature zygotic embryo in a berberis amurensis somatic embryo induction key period is selected as an explant, and casein hydrolysate is added into the culture medium to perform tissue induction on the explant, so that a berberis amurensis embryonic callus is obtained, and the induction rate is high. The callus obtained by differentiation can continuously keep the embryogenic property, and can be further differentiated to induce plant regeneration.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a somatic embryo induction medium and differentiation medium of Phellodendron amurense, and a corresponding tissue culture method. Background technique [0002] Cortex Phellodendron (Phellodendron amurense Rupr.) is a dioecious plant of the genus Phellodendron amurense in Rutaceae. Because of its medicinal and timber dual economic value, it has been massively felled, resulting in a sharp decline in resources. It has now been listed as a national first-class precious tree species, a national second-class protected tree species and a vulnerable species. However, due to artificial excavation and improper utilization, its wild resources have been seriously damaged. [0003] Plant somatic embryogenesis technology is one of the most important means of rapid plant reproduction, which can obtain a large number of vegetative propagules in a short period of time, which is of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张玲张东来田新华
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE
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