Davidia involucrata vitro embryo tissue culture method

Abstract

The present invention provides a Davidia involucrata vitro embryo tissue culture method, comprising: soaking fresh Davidia involucrata vitro embryo in a GA3 solution with the concentration of 8-15 mM at 20-30 DEG C for 1.5-2.5 hours; performing cleaning, disinfecting and sterilizing; and then inoculating the Davidia involucrata vitro embryo in a 1 / 2MS culture medium for culturing. The culture medium comprises: 1 / 2MS, 0.3-0.7 mg / L of 6-BA, 2-5 mg / L of IBA, 25-35 g / L of sucrose and 6-7 mg / L of agar. The present invention can break the dormancy of Davidia involucrata vitro embryo and improve the seedling rate of culture of the Davidia involucrata vitro embryo tissue.

Description

technical field

[0001] The invention relates to the field of isolated embryo tissue culture. More specifically, the present invention relates to a method for culturing in vitro embryo tissue of Davidia involucrata. Background technique

[0002] Davidia involucrata is known as "living fossil of plants", and it is a treasure among the eight first-class national key protected plants. However, due to forest felling and destruction and excavation of wild seedlings for planting, the number is small and the distribution range is shrinking day by day. Therefore, in addition to strengthening protection, it is also necessary to actively carry out introduction cultivation and breeding experiments, carry out artificial afforestation, and expand its distribution area. Tissue culture of in vitro embryos is a proven asexual reproduction technique in the field of cultivating seedlings. However, the isolated embryos of Davidia involucrata can easily form callus or even die during the cultur...