Davidia involucrata vitro embryo tissue culture method
A technology of tissue culture and Davidia involucrata, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as callus and death, and achieve the effect of improving the seedling rate
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example 1
[0030] A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:
[0031] Fresh in vitro dovetail embryos were treated with GA at a concentration of 8 mM at 20 °C 3 Soak in the solution for 1.5 hours, wash, and then inoculate the isolated embryos of Davidia involucrata into the culture medium;
[0032] Wherein, the medium includes: 1 / 2MS, 0.3mg / L of 6-BA, 2mg / L of IBA, 25g / L of sucrose and 6mg / L of agar.
[0033] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.
[0034] In the method for culturing in vitro embryo tissue of Davidia involucrata, the cleaning method is: washing in running water for 0.5 hours.
[0035] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cultivating t...
example 2
[0044]A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:
[0045] Fresh in vitro dovetail embryos were treated with GA at a concentration of 15 mM at 30 °C 3 Soak in the solution for 2.5 hours, wash, and then inoculate the in vitro embryos of Davidia involucrata into the culture medium;
[0046] Wherein, the medium includes: 1 / 2MS, 0.7mg / L of 6-BA, 5mg / L of IBA, 35g / L of sucrose and 7mg / L of agar.
[0047] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.
[0048] In the method for culturing in vitro embryo tissue of Doveia involucrata, the cleaning method is as follows: washing in running water for 1.5 hours.
[0049] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cul...
example 3
[0058] A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:
[0059] Fresh in vitro dovetail embryos were treated with GA at a concentration of 10 mM at 25 °C 3 Soak in the solution for 2 hours, wash, and then inoculate the in vitro embryos of Davidia involucrata into the culture medium;
[0060] Wherein, the medium includes: 1 / 2MS, 0.5mg / L 6-BA, 3mg / L IBA, 30g / L sucrose and 6.5mg / L agar.
[0061] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.
[0062] In the method for culturing in vitro embryo tissue of Doveia involucrata, the cleaning method is as follows: washing in running water for 1 hour.
[0063] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cultivating the i...
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