Davidia involucrata vitro embryo tissue culture method

A technology of tissue culture and Davidia involucrata, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as callus and death, and achieve the effect of improving the seedling rate

Inactive Publication Date: 2015-10-14
邓志军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture of in vitro embryos is a proven asexual reproduction technique in the field of cultivating seedlings. However, the isolated embryos of Davidia involucrata can easily form callus or even die during the culture process. There is dormancy, and conventional culture methods cannot break its dormancy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0030] A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:

[0031] Fresh in vitro dovetail embryos were treated with GA at a concentration of 8 mM at 20 °C 3 Soak in the solution for 1.5 hours, wash, and then inoculate the isolated embryos of Davidia involucrata into the culture medium;

[0032] Wherein, the medium includes: 1 / 2MS, 0.3mg / L of 6-BA, 2mg / L of IBA, 25g / L of sucrose and 6mg / L of agar.

[0033] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.

[0034] In the method for culturing in vitro embryo tissue of Davidia involucrata, the cleaning method is: washing in running water for 0.5 hours.

[0035] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cultivating t...

example 2

[0044]A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:

[0045] Fresh in vitro dovetail embryos were treated with GA at a concentration of 15 mM at 30 °C 3 Soak in the solution for 2.5 hours, wash, and then inoculate the in vitro embryos of Davidia involucrata into the culture medium;

[0046] Wherein, the medium includes: 1 / 2MS, 0.7mg / L of 6-BA, 5mg / L of IBA, 35g / L of sucrose and 7mg / L of agar.

[0047] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.

[0048] In the method for culturing in vitro embryo tissue of Doveia involucrata, the cleaning method is as follows: washing in running water for 1.5 hours.

[0049] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cul...

example 3

[0058] A method for culturing in vitro embryo tissue of Davidia involucrata, comprising:

[0059] Fresh in vitro dovetail embryos were treated with GA at a concentration of 10 mM at 25 °C 3 Soak in the solution for 2 hours, wash, and then inoculate the in vitro embryos of Davidia involucrata into the culture medium;

[0060] Wherein, the medium includes: 1 / 2MS, 0.5mg / L 6-BA, 3mg / L IBA, 30g / L sucrose and 6.5mg / L agar.

[0061] The method for culturing in vitro dovetail embryo tissue, the fresh in vitro dovetail embryo is as follows: the cotyledon of the embryo is immediately cut off after the embryo is stripped from the fresh dovetail seed, and fresh in vitro dovetail embryo is obtained. Embryo.

[0062] In the method for culturing in vitro embryo tissue of Doveia involucrata, the cleaning method is as follows: washing in running water for 1 hour.

[0063] In the described method for culturing in vitro embryos of Davidia involucrata, the specific process of cultivating the i...

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Abstract

The present invention provides a Davidia involucrata vitro embryo tissue culture method, comprising: soaking fresh Davidia involucrata vitro embryo in a GA3 solution with the concentration of 8-15 mM at 20-30 DEG C for 1.5-2.5 hours; performing cleaning, disinfecting and sterilizing; and then inoculating the Davidia involucrata vitro embryo in a 1 / 2MS culture medium for culturing. The culture medium comprises: 1 / 2MS, 0.3-0.7 mg / L of 6-BA, 2-5 mg / L of IBA, 25-35 g / L of sucrose and 6-7 mg / L of agar. The present invention can break the dormancy of Davidia involucrata vitro embryo and improve the seedling rate of culture of the Davidia involucrata vitro embryo tissue.

Description

technical field [0001] The invention relates to the field of isolated embryo tissue culture. More specifically, the present invention relates to a method for culturing in vitro embryo tissue of Davidia involucrata. Background technique [0002] Davidia involucrata is known as "living fossil of plants", and it is a treasure among the eight first-class national key protected plants. However, due to forest felling and destruction and excavation of wild seedlings for planting, the number is small and the distribution range is shrinking day by day. Therefore, in addition to strengthening protection, it is also necessary to actively carry out introduction cultivation and breeding experiments, carry out artificial afforestation, and expand its distribution area. Tissue culture of in vitro embryos is a proven asexual reproduction technique in the field of cultivating seedlings. However, the isolated embryos of Davidia involucrata can easily form callus or even die during the cultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 邓志军蒲鑫胡晓芳罗世家
Owner 邓志军
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