3 results about "Cell cycle control" patented technology

Gene amplifications are an oncogenic driver utilized by many forms of cancer in tumor development or treatment relapse. Promoting genomic instability or the proclivity to propagate genomic alterations through acquired defects in DNA repair machinery, replication licensing, or cell cycle control, gene amplifications not only drive oncogenesis, but also afford an opportunity for therapeutic exploitation. Here, CRISPR-Cas9 nickases are disclosed which selectively promote cancer cell death in a gene amplification-dependent manner. For example, CRISPR- Cas9 nickases generate a lethal number of highly toxic single-ended double-strand breaks within the genome of proliferating cancer cells harboring amplified genomic loci during DNA replication. Cas9 nickases may serve as a tumor-selective therapeutic that mitigates the collateral damage observed with conventional chemoradiotherapies and avoids chemoresistance.

The invention relates to a modified animal cell and a method of producing the modified animal cell having a genetic modification in one or more genes associated with genome surveillance, cell cycle control and / or cell death control. The animal cell is selected from an animal species suitable for human or animal consumption. The animal cell generally has a genetic modification in the RB1, TP53 or RAS genes.

The invention discloses a method for predicting OPU-IVF blastocyst development and embryo production performance by identifying a multi-gene expression pattern in an oocyte and a cumulus cell, which comprises the following steps: detecting expression patterns of mitochondrial functional genes ISCU and IGF2BP3 in the oocyte and an oocyte-cumulus cell interaction gene CTNND1; the method further comprises the step of detecting expression modes of an oxidative phosphorylation and metabolism regulation pathway SOX4, a gene KLF12 participating in cell cycle control and apoptosis regulation, an oxidative phosphorylation and metabolism regulation pathway OGT and an oocyte marker FST in cumulus cells. The expression modes of the genes can predict the embryonic development potential of oocytes after in-vitro maturation and in-vitro fertilization, important genetic information prediction is provided for predicting the reproductive performance of livestock, especially applying the in-vitro fertilization technology to embryo production of large livestock, and the breeding and breeding process of high-value livestock populations is promoted.