The invention relates to a gel formula for lubricating, cleaning vagina and inhibiting bacteria and a preparation method thereof. The raw materials for preparing active ingredients of the gel formulacomprise dittany bark, ash bark, dried alum, oldenlandia diffusa, frankincense, chinaberry bark, catechu, lily, angelica dahurica, cynanchum atratum, white poria cocos, mint, chitosan, lactobacillus salivarius powder and lactic acid powder. The raw materials for preparing the active ingredients are reasonable and scientific in compatibility, the drug effects complement each other, a good synergistic effect can be generated, and the prepared product has the effects of clearing away heat and toxic materials, cooling blood, inhibiting and disinfecting bacteria, killing parasites to relieve itching and nourishing the vagina, can achieve good treatment and prevention effects on gynecological diseases, and is beneficial to vaginal lubrication and female sexual life quality improvement.