Preparation for antibody of antineoplastic specificity marker protein TS/MEDP and use thereof

Abstract

The invention discloses the specificity marker protein TS / MEDP antibody preparation and the purpose. The molecular biology technology is utilized, and a new gene is cloned in a stomach cancer cell and is named as TS / MDEP. A polyclonal antibody for preventing the TS / MDEP is prepared, and a high specificity antibody is acquired through the antibody purification technology. The expression characteristics of the TS / MDEP gene in a tumor cell line and a tumor cell tissue are verified from the mRNA and protein level, the expression in 14 tumor cell lines including the tumor cell line is determined, the dependance of cell cycle is assumed, the excess expression exists in stomach cancer, breast cancer and cervical cancer tissues, no expression is in the corresponding normal tissues, and the expression exists in an embryo tissue. The high expression of the TS / MDEP in the embryo tissue with exuberant cell proliferation is validated, after growing up, the gene is closed, and the high expression exists in the tumor cells. The TS / MDEP has the seasonal specificity expression to clew the occurrence and the development of the cell cycle control abnormity caused by the high expression of the TS / MDEP. The obtained antibody for preventing the TS / MDEP can be used to monitor the cell cycle advancement and the clinical biological behavior of the gastroenteric tumor.

Description

technical field [0001] The present invention relates to various cell and molecular biology technologies, including cell synchronization technology, mRNA differential display technology, RACE technology, prokaryotic expression protein, RT-PCR, mRNA in situ hybridization, polyclonal antibody preparation, immunochemistry, Western blot, Flow cytometry, RNAi and animal experiments. . Background technique [0002] Gastric cancer ranks second among tumor-related diseases in the world, and is the most common malignant tumor in Asian populations, seriously endangering human health. People expect to clarify the mechanism of occurrence and development of gastric cancer, so as to effectively prevent and treat this malignant tumor. Although a large number of studies have confirmed that the occurrence of gastric cancer is related to many factors including diet, environment, and bacterial or viral infection, such as Helicobacter pylori infection, in recent years, molecular mechanism stud...

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Problems solved by technology

Although the discovery of Cyclin and CDK has clarified the basic regulatory mechanism of the cell cycle, there is also a lot of understanding of the G1-S phase checkpoint, such as P53, P21, GADD45, TGF-β, P27, P16, Rb, and E2F participate in the G1-S phase checkpoint. The regulation of S phase checkpoint, but we are still far from understanding the detailed molecular regulation mechanism of cell cycle regulation

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