81 results about "Differential display" patented technology

The invention concerns a watch with a mechanical movement of the type comprising at least two regulating systems (SR1, SR2) each including a mechanical oscillator and an escapement. The movement includes as many sub-assemblies (SE1, SE2) as there are regulating systems, each of said sub-assemblies including a regulating system (SR1, SR2), a barrel (B1, B2) and a going train transmitting energy from the barrel to the regulating system inside the sub-assembly. A differential display gear (Da) connects the barrel or the going train of each sub-assembly to the display (A) to average out the rate of the two sub-assemblies. In a preferred embodiment, the two sub-assemblies are mounted on a common rotating support (5) and their regulating systems are tourbillons which make an orbital movement about the centre of the watch dial.

The present invention relates to protein separation systems and methods capable of resolving and characterizing large numbers of cellular proteins. In particular, the present invention provides a novel mass mapping system and methods for the differential display of proteins. The present invention further provides novel methods for displaying differential protein expression between two samples. In particular, the present invention provides novel method of mapping differential expression of proteins in non-cancerous, pre-cancerous, and cancerous cells.

Genes whose expression differ between that in eosinophils collected from atopic dermatitis patients of the exabartation stage and those of the remission stage were searched via a differential display method. As a result, NOR-1 (MINOR) gene was successfully identified whose expression significantly increased in eosinophils of patients in the remission stage, a stage associated with a decrease of eosinophils. The present inventors discovered that the gene can be successfully employed in testing for allergic diseases and screening for candidate compounds for therapeutic agents.

The invention relates to a knowledge graph display method and device, computer equipment and a storage medium in the field of big data. The method comprises the following steps: obtaining knowledge maps under a plurality of spectrum views, and displaying the knowledge maps; Wherein the knowledge graph comprises a plurality of entities corresponding to the pedigree view angles and a plurality of relation types; Receiving a map screening condition; Screening entities and relationship types of the knowledge map according to the map screening conditions; And carrying out differential display on the screened entities and the relationship types. By adopting the method, the relationship between companies in a group enterprise can be quickly and effectively queried.

The invention concerns a watch with a mechanical movement of the type comprising at least two regulating systems (SR1, SR2) each including a mechanical oscillator and an escapement. The movement includes as many sub-assemblies (SE1, SE2) as there are regulating systems, each of said sub-assemblies including a regulating system (SR1, SR2), a barrel (B1, B2) and a going train transmitting energy from the barrel to the regulating system inside the sub-assembly. A differential display gear (Da) connects the barrel or the going train of each sub-assembly to the display (A) to average out the rate of the two sub-assemblies. In a preferred embodiment, the two sub-assemblies are mounted on a common rotating support (5) and their regulating systems are tourbillons which make an orbital movement about the center of the watch dial.

Cellophane wrapping (CW) of hamster pancreas induces proliferation of duct epithelial cells followed by endocrine cell differentiation and islet neogenesis. Using the mRNA differential display technique a cDNA clone expressed in cellophane wrapped but not in control pancreata was identified. Using this cDNA as a probe, a cDNA library was screened and a gene not previously described was identified and named INGAP.

The present invention relates to novel methods for the identification of antigens recognized by cytotoxic T cells (CTLS) and specific for human tumors, cancers, and infected cells, and the use of such antigens in immunogenic compositions or vaccines to induce regression of tumors, cancers, or infections in mammals, including humans. The invention encompasses methods for induction and isolation of cytotoxic T cells specific for human tumors, cancers and infected cells, and for improved selection of genes that encode the target antigens recognized by these specific T cells. The invention also relates to differential display methods that improve resolution of, and that reduce the frequency of false positives of DNA fragments that are differentially expressed in tumorous, cancerous, or infected tissues versus normal tissues. The invention further relates to the engineering of recombinant viruses as expression vectors for tumor, cancer, or infected cell-specific antigens.

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

Provided are affinity support materials having intermediate binding affinity for biological samples. Among the materials provided by the present invention are hydrophilic solid supports composed of hydrophilic ligands coupled to hydrophilic matrixes which are compatible with biological samples, for example, a cell line, a biological fluid such as blood, or a tissue cell lysate. The ligands may include affinity property groups and hydrophilic groups pendent from a backbone, and be configured to at least partially resolve components of a biological sample. Affinity supports in accordance with the present invention may be used in a variety of techniques and apparatuses to achieve improved separations of complex biological samples and thereby enhance the results of biological sample component fractionations, enrichments, purifications, expression product determinations and comparisons, and other biological sample processing techniques. In addition, the affinity supports may be included in kits useful in processing biological samples.

The present invention relates to a colored perfusion liquid exclusively for ophthalmic surgery. The colored perfusion liquid exclusively for ophthalmic surgery of the present invention includes conventional ophthalmic balanced salt solution and is characterized in that the present invention further contains 0.001 to 0.1mg / ml fluorescein sodium. The present invention firstly proposes the intraocular colored perfusion liquid and a concept of colored surgery, the present invention has the advantages that: the present invention can be filled in the anterior chamber, vitreous body, lens capsular bag and other lacunas, and can form the contrast with the intraocular transparent and semi-permeable membrane tissues, such as, cornea, lens capsule membrane, lens, vitreous body, retina and so on, therefore, the operator can identify such structures easily and the tissues which are difficult to identify become visible, therefore, the surgery becomes easy, the operation is accurate, the time is shorten, the retina, lens capsule membrane and other main structures are protected, the surgical complications are reduced, and better surgical effects are achieved accordingly. In addition, the present invention changes and overcomes the problems that the past localized staining method is time-consuming and needs to rinse, the surgical process is complicated, the operation is fairly troublesome, and the method can not carry out the differential display of the around tissues, that is the scope is too small etc.

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

The invention relates to the technical field of networks, and discloses a content publishing system supporting multiple terminals.The system comprises CMS modules, an acquiring module, a recognizing module, a data calling module and a publishing module; the CMS modules are used for storing to-be-published data, wherein the to-be-published data comprises data in a basic screen and data in a subscreen; the recognizing module is used for recognizing UA information according to a page display request; the data calling module is used for looking up and calling the to-be-published data matched with the terminals from the CMS modules according to the UA information; the publishing module is used for publishing the to-be-published data matched with the terminals to the corresponding terminals.According to the system, when a content publishing system CMS is constructed, a MongoDB is adopted as a storing structure of a base layer, the display content of the different screens is maintained through the same set of page management data, the management efficiency is improved, and differential display and automatic matching display on the different screens are achieved through the system.

The current invention provides methods for the identification of antigens and / or epitopes that are differentially displayed on TAP deficient or TAP impaired cells and are not detectably displayed on normal or TAP proficient cells. The identification and applications of these differentially presented antigens, which in this specification are referred to as TEIPP, T cell Epitopes associated with Impaired Peptide Processing, is a prime object of this invention. The invention also provides peptides comprising a TEIPP epitope obtained from the methods of the invention, which may be applied in medicaments and methods of treatment raising a T cell response against TAP deficient tumor cells or virally infected cells.

Pluripotent cells that are suitable for transplantation therapy, to repair neural damage, are identified, e.g. by differential display, from a gene expression profile for a selected cell, which can be compared with that obtained from a control cell.

The invention provides a stereoscopic graph display method and apparatus, and an electronic device. A stereoscopic graph outline comprises a bottom surface outline and an outline generatrix. The method comprises the steps of displaying a stereoscopic graph on a graphic user interface; receiving a rotating operation instruction for the stereoscopic graph; transforming a display view angle of the stereoscopic graph according to the rotating operation instruction; along with change of the display view angle of the stereoscopic graph, determining a visible part and an invisible part of the bottom surface outline at the current view angle and the position of the outline generatrix in real time; and displaying the outline generatrix according to the position of the outline generatrix, and differentially displaying the invisible part of the bottom surface outline and other parts of the stereoscopic graph outline. By applying the method and the apparatus, the visibility of the bottom surface outline and the position of the outline generatrix of the stereoscopic graph are determined in real time, and in a three-dimensional rotating operation process, the display of the outline generatix of the stereoscopic graph and the differential display of the invisible part of the bottom surface outline and other parts are realized, so that the display of the stereoscopic graph is more intuitive and the user experience is relatively good.

The invention discloses a method of detecting EGFR (epidermal growth factor receptor) gene mutation in urine of a colorectal cancer patient by using digital PCR (polymerase chain reaction). The method comprises the particular following steps: extracting a DNA (deoxyribonucleic acid) template from the urine, preparing ddPCR (differential display polymerase chain reaction) liquid, preparing a droplet, performing PCR amplification, detecting the droplet, analyzing data, and displaying a result, wherein a lysate comprises 10mM of Tris-HCl, 1mM of EDTA (ethylene diamine tetraacetic acid) and 0.5% of SDS (sodium dodecyl sulfonate); a thermal circulation mode of detection comprises the steps of incubating at 95 DEG C for 10min, at 95 DEG C for 15s, at 63 DEG C for 1min (45 cycles in total), and finally putting at 4 DEG C. According to the method, ARMS is combined with the digital PCR; the method solves the problems of complication, time consuming, low sensitivity and the like during EGFR gene mutation detection of the colorectal cancer patient in the prior art by detecting the EGFR mutation in the urine of the patient; the non-traumatic detection in addition to pathologic histology is provided particularly.

The invention provides a primer and probe composition for detecting V600E of a BRAF (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) gene and a detecting method of the primer and probe composition. The primer and probe composition comprises a mutant probe which aims at a V600E site of the BRAF gene and is as shown in SEQ ID NO.1, a wild type probe which aims at the V600E site of the BRAF gene andis as shown in SEQ ID NO.2 and specific primers which aim at the V600E site of the BRAF gene and are as shown in SEQ ID NO.3 to SEQ ID NO.4. Primer designing and probe modification are carried out ona specific zone aiming at the V600E site, a primer and a probe are matched with each other, ddPCR (Differential Display Polymerase Chain Reaction) is combined with a high-specificity primer and probecomposition, extraction of cfDNA (Cell-free DNA), recycle number of PCR and annealing temperature are optimized, all steps are in a synergistic effect, the accuracy, the stability and the sensitivityfor detection are finally increased, and wide application prospect and a market value are obtained.

The present invention relates to novel methods for the identification of antigens recognized by cytotoxic T cells (CTLs) and specific for human tumors, cancers, and infected cells, and the use of such antigens in immunogenic compositions or vaccines to induce regression of tumors, cancers, or infections in mammals, including humans. The invention encompasses methods for induction and isolation of cytotoxic T cells specific for human tumors, cancers and infected cells, and for improved selection of genes that encode the target antigens recognized by these specific T cells. The invention also relates to differential display methods that improve resolution of and that reduce the frequency of false positives of DNA fragments that are differentially expressed in tumorous, cancerous, or infected tissues versus normal tissues. The invention further relates to the engineering of recombinant viruses as expression vectors for tumor, cancer, or infected cell-specific antigens.

The embodiment of the invention provides a function menu display method and system and a related device, which are used for differentially displaying each menu option in a to-be-displayed menu list, so that a user can conveniently and quickly identify common menu options, and the user experience is improved. The method provided by the embodiment of the invention comprises the steps of obtaining ato-be-displayed menu list sent by diagnosis software; determining the priority of each menu option in the menu list to be displayed; and carrying out differential display on menu options with different priorities. According to the embodiment of the invention, the automobile diagnosis equipment can determine the priority of each menu option in the to-be-displayed menu list after acquiring the to-be-displayed menu list sent by the diagnosis software, and differentially display the menu options with different priorities, so that a user can conveniently and quickly identify common menu options, and the user experience is improved.

The invention relates to the field of gene detection, and in particular relates to a method for improving sensitivity of digital PCR and a kit thereof. The method for improving the sensitivity of thedigital PCR comprises at least the steps of: enriching a target nucleic acid sequence in a test sample or a mutant type gene sequence and a wild type gene sequence of a to-be-tested gene mutation sitein a same ratio in a linear range to obtain an enriched template for detecting the target nucleic acid sequence of the to-be-tested gene mutation site for the digital PCR detection. The method significantly improves the situations of a low detection rate and a high false positive of ddPCR (differential display PCR) for detecting the trace amount of the targeted nucleic acid sequence in the test sample, and greatly improves the sensitivity of the ddPCR and the value for use in clinical detection.

Provided is an alarm method for computer hardware configuration changing. System hardware changes are detected in shutdown states which include a shutdown state with a power supply and a shutdown state without a power supply; after the hardware configuration changes, hardware triggers interrupt, platform configuration information is obtained through a BIOS SMI program, a BMC completes the storageand differential display of the configuration information, and the three cooperate to complete the timely and accurate recording of the platform configuration information on a platform body. After thehardware information changes, the BIOS can flexibly and diversely set operation paths according to requirements, and can be set to no operation, or warning information is displayed and the operationis continued, or a system is suspended until an administrator confirms the security and then the system continues to run. The information stored by the BMC enters an EEPROM. After the power is turnedoff, the information can still be saved and does not disappear. A BMC display interface provides a uniform information platform for research staff, testers, and customers. The communication efficiencyis improved, and two configurations are displayed in a differentiated mode, which can remind the customers of the hardware changes.

The present invention relates to multi-phase protein separation methods capable of resolving and characterizing large numbers of cellular proteins, including methods for efficiently facilitating the transfer of protein samples between separation phases. In particular, the present invention provides systems and methods for the differential display of protein samples from multiple cell types. The present invention thus provides improved methods for the analysis of multiple samples containing large numbers of proteins.

The invention relates to a method and device for differentially displaying rebroadcast videos and a storage medium, and the method is used for realizing the differential display of the videos, and comprises the steps: inputting a plurality of videos, synthesizing a plurality of videos in one frame through a transmitting card, and transmitting the synthesized videos in a time-sharing manner; adjusting the phase of shutter opening time of the camera rebroadcasting equipment relative to the synchronous locking signal; adding compensation corresponding to the rebroadcast video into the live video;the invention provides a method for differentially displaying rebroadcast videos. By calculating the final three-color gray value of each pixel point of the on-site video displayed on the display screen for compensation, random movement of the camera rebroadcasting equipment is realized, and the on-site video controller can play the video on the display screen for the camera rebroadcasting equipment to shoot, so that the method is suitable for the camera rebroadcasting equipment to display the on-site video differently.

The invention discloses a method for displaying applications and shortcuts of handheld equipment in different ways. A main screen of the handheld equipment includes a desktop and a main menu, desktop elements include shortcuts and widges, main menu elements include applications, and the startup of the main screen comprises desktop loading and main menu loading. According to the method for displaying applications and shortcuts of handheld equipment in different ways, the handheld equipment adds differential display characteristics to the applications during an application loading process and adds differential display characteristics to the shortcuts during a shortcut loading process; since differential characteristics are added to icons of corresponding applications and shortcuts in the loading processes, after loading, a user can distinguish conveniently and quickly and can avoid various misunderstandings; moreover, a good interaction experience with the user can be achieved.

The present invention relates to an efficient mAb panel-based expression profiling technology platform suitable for global and accurate measurement of proteins, peptides and metabolites in complex mixtures. The platform is comprised of new and well established technologies that are coupled in a unique fashion to provide a novel platform technology for (i) the discovery of differentially displayed elements of complex protein, peptide and metabolite mixtures and (ii) the development of robust mAb based assays that detect the differentially expressed elements.

Embodiments of the application disclose a display control method and device. The display control method comprises steps of changing a ratio of pixels distributed in two directions in an effective display area of at least one display unit of a display system; transmitting light emitted by each pixel in the effective area of each display unit of the at least one display unit in a visual angle range via a lens corresponding to the display unit in the display system; and displaying to-be-displayed content via the display system after changing. The two directions comprise a first direction and a second direction which are parallel to the display unit and mutually orthogonal. The application achieves differential display of visual angel information in two mutually orthogonal directions.

The present invention relates to protein separation systems and methods capable of resolving and characterizing large numbers of cellular proteins. In particular, the present invention provides a novel mass mapping system and methods for the differential display of proteins. The present invention further provides novel methods for displaying differential protein expression between two samples. In particular, the present invention provides novel method of mapping differential expression of proteins in non-cancerous, pre-cancerous, and cancerous cells.

The invention discloses a design implementation method based on a power grid GIS platform map display style model base. The method comprises the steps that (1) data formats and coordinate systems are unified; (2) navigation data is fused with data according to the scale of 1:2,000 and the scale of 1:500 respectively, and the data is imported into a File Geodatabase; (3) POIs and roads are extracted and subdivided according to element codes, point annotations are subjected to layered extraction according to GEOCODE properties of a point annotation layer, annotations of the POIs obtained after subdivision, traffic, administrative division and the like are subjected to differential display, and a display scale is set for each layer; (4) annotation setting and symbolic setting are completed, an optimized map is saved, an mxd is generated, and relative paths are saved; (5) service is published, and slicing is tried; and (6) a standardized map rendering scheme is elaborated in the form of a standard or norm document, and finally the power grid GIS platform map display style model base is formed.