Application of berberine in preparing tumor radio sensitization medicine

A technology of tumor radiation and berberine, which is applied in the field of tumor radiotherapy sensitizer drugs, can solve the problems of no report on the application of berberine radiotherapy sensitizer drugs, the inability to produce anti-tumor effects, and toxicity, so as to improve the therapeutic effect and reduce toxicity. Side effects, effects of reducing radiation dose

Inactive Publication Date: 2010-09-01
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have also found that large doses of berberine have anti-tumor effects, can inhibit the proliferation of colon cancer cells, inhibit the growth of esophageal cancer, breast cancer and prostate cancer cells, but high doses of berberine will also cause toxicity, although reducing the dose can eliminate toxicity , but the antitumor effect cannot be produced when the dose is small
At the same time, there is no report on the application of berberine as a radiosensitizer.

Method used

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  • Application of berberine in preparing tumor radio sensitization medicine
  • Application of berberine in preparing tumor radio sensitization medicine
  • Application of berberine in preparing tumor radio sensitization medicine

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Experimental program
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Embodiment

[0034] (1) Materials: Human breast cancer cell lines MDA-MB-231 and MCF-7 were purchased from the American Cell Collection Center (ATCC), and were preserved and cultivated by the inventor's laboratory; D-MEM medium dry powder, calf serum , trypsin powder was purchased from Gibco; standard protein molecular weight, SDS-PAGE loading buffer, RIPA protein lysate, TE electrophoresis buffer, 10× transfer solution, 30% Acry-Bis, Tris-Hcl, ammonium persulfate (AP), SDS, tetramethylethylenediamine (TEMED), propidium iodide (PI) were purchased from Jiangsu Beyantian Biotechnology Research Institute; dimethyl sulfoxide (DMSO), agarose and Tween-20 were from Shanghai High-tech Bioengineering Co., Ltd.; caspase-3, bcl-2, bax, cyclin D1 and other antibodies were purchased from Santa Cruzs Biotechnology Company of the United States; RNase A and 10 μg / mL proteinase K were purchased from Sigma Aldrich Company of the United States.

[0035] (2) Cell culture: MDA-MB-231 and MCF-7 cells were cult...

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Abstract

The invention discloses a tumor radio sensitization medicine which has an active component of berberine. Proved by research: the berberine has the following efficacy of: 1, breast cancer cells are treated by berberine, which can induce dose-dependently G0/G1 stage cell retardation and apoptosis; 2, the berberine remarkably reduces the expression level of cell cycle control associated protein cyclin B and apoptosis resisting protein Bcl-2 and increases the expression level of apoptins Bax and caspase-3; and 3, the breast cancer cells treated by the berberine remarkably improve the sensitiveness of the cells to radiotherapy. The tumor radio sensitization medicine can improve the treatment effect of tumor radiotherapy, lower the exposure dose of the radiotherapy of a tumor patient, and lower the toxic and side effects brought by the radiotherapy, thereby improving the living quality of patients.

Description

technical field [0001] The invention belongs to the field of sensitizing drugs for tumor radiotherapy, and in particular relates to the application of berberine in the preparation of sensitizing drugs for tumor radiotherapy. Background technique [0002] In the prior art, commonly used radiotherapy sensitizing drugs include: [0003] (1) MISO (Misonodaxole): MISO is a fully affirmed hypoxic cell sensitizer in the clinical trial stage. [0004] (2) Kanglaite: It is an injection made from the traditional Chinese medicine coix seed. The drug has been widely used in the treatment of various malignant tumors, but also a radiosensitizer. [0005] (3) SR-2508 (Etanidacole): SR-2508 is an amide derivative of nitroimidazole. Its sensitizing potency is similar to that of MISO, but its toxicity is lower. The maximum tolerable total dose is about 3 times that of MISO. SR-2508 also has a sensitizing effect on chemotherapeutic drugs. [0006] (4) AK-2123: AK-2123 is a nitroimidazole c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4375A61P35/00
Inventor 樊赛军
Owner SUZHOU UNIV
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