5 results about "Hypoxic cell" patented technology

The invention relates to hypoxia response therapeutic VEGFA (vascular endothelial growth factor A) mRNA (messenger Ribonucleic Acid) and application thereof. The mRNA comprises a 5'end cap, a 5 'UTR (Untranslated Region) containing a Kozak sequence, VEGFA ORF (Open Region Fragment), a 3' UTR containing an RNA (Ribonucleic Acid) binding protein HuR binding site, a RhoBAST fluorescent aptamer at the tail end of the 3 'UTR and a PolyA tail. According to the design, through a HuR hypoxia-dependent nuclear plasma transport mechanism, the stability of mRNA in hypoxic cells is improved by 2.5 times, the protein expression quantity is improved by 2 times, and proliferation of endothelial cells is remarkably promoted. Further disclosed is a VEGFA mRNA delivery system based on engineered extracellular vesicles (EVs): EVs surface modified kidney targeting peptide LTH. The system is efficiently accumulated in ischemic kidneys, VEGFA expression is remarkably enhanced, and abnormal vascular hyperplasia of external renal organs is avoided. Animal experiments prove that the survival rate of a renal ischemia-reperfusion injury model can be increased to 100% by intravenous injection of drug-loaded EVs, and the capillary density is remarkably increased 7 days after treatment. The invention is suitable for treating ischemic diseases, organ ischemia reperfusion injury or chronic wounds and the like, and has the advantages of accurate targeting, hypoxia response, high stability and safety.

Provided are a hyaluronic acid compound for resisting cell hypoxia injury, a preparation method therefor, and use thereof. The hyaluronic acid compound comprises hyaluronic acid with an average molecular weight in the ten-thousands range and hyaluronic acid with an average molecular weight in the hundred-thousands range according to a volume ratio of 1:3 to 3:1. Hyaluronic acid can inhibit hypoxia-induced overactivation of HIF-1α, reduce the reactive oxygen level in hypoxic cells, and reduce the level of inflammatory factors in the supernatant of hypoxic cells, thereby alleviating hypoxia-induced cell apoptosis. Therefore, hyaluronic acid can be used in the treatment of cell hypoxia injury. Compared with hyaluronic acid of a single molecular weight, the obtained hyaluronic acid compound has a synergistic effect.

The present application relates to a kind of hypoxic fluorescent probe and its preparation method and application, belong to the field of biotechnology.The present application designs new type of hypoxic fluorescent probe, and further develops its in situ imaging application mode in the field of neurobiology, the hypoxic fluorescent probe can effectively respond to hypoxic cell, tissue, and can penetrate blood-brain barrier, release fluorescence at the place of hypoxic brain injury with azo bond broken, has the potential of clinical diagnosis neonatal hypoxic-ischemic encephalopathy.

The invention relates to the technical field of fluorescent probe preparation, in particular to a small-molecule fluorescent probe and a preparation method thereof. The structural formula of the micromolecular fluorescent probe is shown in the specification. The micromolecular fluorescent probe provided by the invention can be used for detecting hydrogen peroxide, can respond to hypoxia cells and tissues, realizes in-situ fluorescence imaging, and can effectively penetrate through a blood brain barrier.

A kind of laboratory device of long time low oxygen culture cell of flexible stability, assembly structure is simple, each block is flexibly detached in use, low oxygen gas flow rate is stable in culture, environment is sterile environment, humidity is sufficient, realize the stable culture of cell in low oxygen environment for long time.It includes gas cylinder (1), bacteria filter (3), cell low oxygen incubation chamber (5);Cell low oxygen incubation chamber includes water tank (51), metal bucket (52), glass cover (53), sealing rubber ring (54);Water tank is equipped with electric plug (514), predetermined temperature is obtained by electrification heating to water in water tank, water tank has the heat preservation cover with hole;Metal bucket, glass cover and sealing rubber ring form closed structure, the lower part of metal bucket body is immersed in the water of water tank, the upper part of metal bucket body passes through the hole of heat preservation cover and exposes water tank.