Device for cell intermittent hypoxia experiment

Abstract

The invention relates to a device for a cell intermittent hypoxia experiment. The device comprises a cell cultivation case formed by a closed case body, a cell cultivation dish, a humidity and temperature control device, an oxygen gas source, a carbon oxygen gas source, a nitrogen gas source and a main controller. The gas sources are respectively connected with the cell cultivation case through corresponding gas conveying pipelines, control valves are arranged in the gas conveying pipelines, and the main controller controls the control valves to periodically adjust the oxygen content in the cell cultivation case to be changed between a high state and a low state. Due to the fact that the device is provided with the humidity and temperature control device, environment in the case body is made to be suitable for the growth of cells, the main controller controls the control valves to periodically adjust the oxygen content in the cell cultivation case to be changed between the high state and the low state to form a periodic hypoxia environment, the periodic hypoxia environment can be created, and then the study of intermittently-hypoxic cells is carried out.

Description

technical field [0001] The invention relates to a cell culture experimental device, more precisely to a cell intermittent hypoxia experimental device. Background technique [0002] Sleep apnea syndrome (SAS), especially obstructive sleep apnea-hyponea syndrome (OSAHS), is a chronic sleep-respiratory disorder with a high incidence, which seriously affects people's physical and mental health. SAS refers to 7 hours of sleep per night, with more than 30 repeated episodes of apnea or sleep disordered breathing time index (respiratory impairment time index, RIT, that is, the average number of sleep apnea + hypopnea per hour) ≥ 5 times. This leads to recurrent hypoxemia and hypercapnia, which can have various effects on the body: such as drowsiness, dizziness, fatigue, abnormal mental behavior, and morning headaches. As the disease progresses, severe complications such as pulmonary hypertension, pulmonary heart disease, respiratory failure, hypertension, and arrhythmia may occur....

AI Technical Summary

Problems solved by technology

However, the existing intermittent hypoxic animal breeding cabin does not have a constant temperature and constant humidity environment, and the existing two-gas and three-gas cell incubators cannot perform frequent exchange and humidity control, so they do not have the ability to perform intermittent hypoxic cells. There is an urgent need to develop an experimental device for intermittent hypoxia in cells

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