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Human autogenous siRNA sequence, its application and screening method

A DNA sequence, endogenous technology, applied in the field of bioinformatics

Inactive Publication Date: 2007-01-31
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But human endogenous siRNA has not been found in the world so far, so the disclosure of human endogenous siRNA and its target gene by the present invention is very important, it will greatly promote the research of small RNA, and provide valuable research on the mechanism of action of RNA data and provide new avenues for human disease research

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  • Human autogenous siRNA sequence, its application and screening method
  • Human autogenous siRNA sequence, its application and screening method
  • Human autogenous siRNA sequence, its application and screening method

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Embodiment Construction

[0013] 1. Terminology

[0014] In the context of the present invention, the term "double-stranded oligonucleotide" as used is a duplex of polymers or oligomers containing nucleotides. Such as a double-stranded RNA molecule (dsRNA), a double-stranded DNA molecule (dsDNA), a double-stranded sRNA-cDNA hybrid molecule. The term further includes oligonucleotides composed of natural nucleotides, sugars and covalent linkages between nucleotides, as well as modified or non-natural nucleotides. Each of these oligomer types and their numerous derivatives have been extensively reported. Those modified or substituted nucleotides are often superior to natural nucleotides. If they are used to synthesize corresponding oligonucleotides, their products may have stronger enzymatic resistance and better uptake by cells performance and higher affinity performance with its target nucleic acid.

[0015] As used herein, the terms "double-stranded RNA molecule (dsRNA), double-stranded DNA molecule...

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Abstract

The invention relates to a computer algorithmic language and relative application software. It could rapidly select and predict internal source siRNA gene and siRNA molecule. Especially, it relates to an entire finding method for internal source siRNA gene and internal source siRNA molecule, and gaining 255 new siRNAs and target mRNAs from coding gene intron. The siRNA molecules could be used to research develop, differentiation and growth of cell, organization and organic, discuss the function and express adjusting network and discuss function of genes. It also could be used to develop medicines to prevent and cure kinds of disease.

Description

technical field [0001] The present invention relates to bioinformatics, and more specifically, the present invention relates to a method for screening siRNA (small interfering RNA, small molecule interfering RNA). The present invention also relates to human endogenous siRNA sequences screened by the method and applications thereof. Background technique [0002] Micro RNAs (miRNAs) are a large class of ~22 nucleotide long non-coding single-stranded RNA molecules. Evolutionarily, they are widespread in cells from plants, nematodes to humans [1] . The earliest discovery was lin-4 and its target mRNA, lin-14 [2] . Lee et al [2] In 1993, the lin-4 gene was cloned in C.elegans (nematode) by the classic positional cloning method, and through site-directed mutation, it was found that lin-4 did not encode a protein, but produced a small RNA molecule. This small RNA molecule can interact with a specific region of the 3' untranslated end of its target mRNA in an incompletely comp...

Claims

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Application Information

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IPC IPC(8): G06F19/00C12Q1/68C07H21/02A61K31/7088A61K48/00A61P35/00A61P3/00G06F19/18
Inventor 殷勤伟戴正华谷同军冯圣中丘振戈
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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