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Continuous operation method for extraction and separation of stem cells

An operation method and stem cell technology, applied in the field of stem cell separation, can solve problems such as delay in research time, inconvenient operation, long time, etc., and achieve the effect of keeping the working environment dry, simple and ingenious in structure, and rapid extraction

Inactive Publication Date: 2020-11-10
荣丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In reality, to separate stem cells from embryos, researchers need to slowly scrape the tissue with a knife, which is laborious and time-consuming, and it is easy to dirty hands and workbenches. Before separating on the transfer separator, the surface of the reagent tube for collecting embryonic tissue must be wiped clean. To avoid dirtying the separator, its operation is cumbersome and inconvenient, which affects the smooth research work of researchers. In addition, after the existing separator rotates and separates, the reagent tube can only be removed after it stops, so as not to affect the refusion inside the reagent tube. This method requires more time, delay in research

Method used

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  • Continuous operation method for extraction and separation of stem cells
  • Continuous operation method for extraction and separation of stem cells
  • Continuous operation method for extraction and separation of stem cells

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Embodiment 1

[0067] The invention provides a continuous operation method for extracting and separating stem cells, comprising the following steps:

[0068] 1. The stage of extraction and collection of embryonic stem cells:

[0069] S1. Cut the prepared animal embryos into slices in advance, then move the fan-shaped plate 125 of the embryonic stem cell extraction and separation equipment outward to open the top port of the crushing cylinder 120, and then put the embryo slices into the crushing cylinder 120;

[0070] S2, then start the servo motor 121 to make it work, drive the output shaft and the blade 122 at its bottom to rotate, and then smash the embryo slices into pieces;

[0071] S3, restart the electric push rod 320 to make the piston rod extend and work, and then rise up against the extension column 123 and the crushing cylinder 120 as a whole, so that the crushed embryos are scattered into the bottom of the extraction cylinder 110;

[0072] S4, then start the deceleration motor 31...

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Abstract

The invention discloses a continuous operation method for extraction and separation of stem cells. The continuous operation method comprises the following steps: crushing and extracting embryo piecesin an extraction device, classifying and separating embryo powder in a separation device, quickly and stably stopping the separation device through a speed reducing mechanism, and taking thoroughly separated reagent tubes. Collected embryo pieces are poured into the arranged extraction device and crushed into powder by a smashing barrel, then centrifugal force is generated through rotation of an extraction barrel, tissue with different components is separated out and isolated and collected, embryo tissue can be conveniently and rapidly extracted, and the working environment is kept dry and comfortable. The tissue and liquid in the reagent tubes are separated through centrifugal force produced through rotation of the separation barrel. The speed reducing mechanism is arranged below the separation barrel, so that the separation device can be stopped quickly and stably in a short time, and a researcher can take the reagent tubes for research in a time-saving manner.

Description

technical field [0001] The invention relates to the technical field of stem cell separation, in particular to a continuous operation method for extracting and separating stem cells. Background technique [0002] Stem cells are highly undifferentiated cells, which have the characteristics of unlimited proliferation, self-renewal and multi-directional differentiation. They can be cultured, expanded and established in vitro, and can also be induced to differentiate into various tissue cells under appropriate conditions. Stem cells are widely distributed in animal bone marrow, blood, various organs and embryos, especially embryonic stem cells are the most abundant and primitive, and can be differentiated into various organs and tissues, which have medical treatment and research value. In reality, to separate stem cells from embryos, researchers need to slowly scrape the tissue with a knife, which is laborious and time-consuming, and it is easy to dirty hands and workbenches. Bef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0735C12M1/33C12M1/00B02C18/10
CPCB02C18/10C12M33/10C12M45/02C12M45/05C12M47/04C12N5/0606C12N2509/10
Inventor 荣丽
Owner 荣丽
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