A method for tissue culture and rapid propagation of Ilex serrata using stem segments as explants

A technology of tissue culture and rapid propagation of Ilex serrata, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve restrictions on promotion and propagation, low rooting rate of Ilex serrata tissue culture seedlings, and transplant survival rate low level problem

Active Publication Date: 2021-08-10
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low rooting rate of Ilex serrata tissue culture seedlings in the current tissue culture method, the transplanting survival rate is low, which limits its large-scale promotion and propagation.

Method used

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  • A method for tissue culture and rapid propagation of Ilex serrata using stem segments as explants
  • A method for tissue culture and rapid propagation of Ilex serrata using stem segments as explants
  • A method for tissue culture and rapid propagation of Ilex serrata using stem segments as explants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A kind of Ilex serrata tissue culture rapid propagation method using stem segment as explant provided by the present embodiment comprises:

[0024] (1) Select holly branches and wash them with washing powder for 20 minutes, then rinse them with running water for 10 minutes to remove surface dirt. Transfer to an ultra-clean workbench and rinse with sterile water once. Then soak in 75% alcohol for 5 minutes, then soak in 8% sodium hypochlorite for 15 minutes, and finally rinse with sterile water for 5-6 times, and set aside.

[0025] (2) Branches are cut into budding stem segments of about 1 cm, and inoculated on MS medium supplemented with 2.28 μM ZT for 8 weeks of proliferation and elongation culture;

[0026] (3) Induction of regenerated roots for 8 weeks on 1 / 2MS medium containing 4.14 μM IBA;

[0027] (4) Transplanted into the cultivation medium and placed on the greenhouse seedbed to grow under the spraying system, the transplanting survival rate reached 93.5%.

Embodiment 2

[0029] A kind of Ilex serrata tissue culture rapid propagation method using stem segment as explant provided by the present embodiment comprises:

[0030] (1) Select holly branches and wash them with washing powder for 20 minutes, then rinse them with running water for 10 minutes to remove surface dirt. Transfer to an ultra-clean workbench and rinse with sterile water once. Then soak in 75% alcohol for 5 minutes, then soak in 8% sodium hypochlorite for 15 minutes, and finally rinse with sterile water for 5-6 times, and set aside.

[0031] (2) branch is cut into the band bud stem segment of about 1cm, is inoculated on the MS medium that adds 4.56 μ M ZT and carries out 60 days of proliferation and elongation culture;

[0032] (3) On the 1 / 2MS medium containing 4.14 μ M IBA, carry out the induction of the regenerated root of 60 days;

[0033] (4) Transplanted into the cultivation medium and placed in the greenhouse seedbed to grow under the spraying system, the transplanting s...

Embodiment 3

[0035] A kind of Ilex serrata tissue culture rapid propagation method using stem segment as explant provided by the present embodiment comprises:

[0036] (1) Select holly branches and wash them with washing powder for 20 minutes, then rinse them with running water for 10 minutes to remove surface dirt. Transfer to an ultra-clean workbench and rinse with sterile water once. Then soak in 75% alcohol for 5 minutes, then soak in 8% sodium hypochlorite for 15 minutes, and finally rinse with sterile water for 5-6 times, and set aside.

[0037] (2) branch is cut into the band bud stem section of about 1cm, is inoculated on the MS medium that adds 9.12 μ M ZT and carries out 50 days of proliferation and elongation culture;

[0038] (3) Induction of regenerated roots for 50 days on 1 / 2MS medium containing 4.14 μM IBA;

[0039] (4) Transplanted into the cultivation medium and placed on the greenhouse seedbed to grow under the spraying system, the transplanting survival rate reached 9...

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Abstract

The invention discloses a tissue culture rapid propagation method of Ilex serrata using stem segments as explants, comprising: step 1, collecting branches of Ilex serrata, cleaning and disinfecting; step 2, cutting the branches of Ilex serrata into stems with buds section, inoculated on the proliferation and elongation medium for proliferation and elongation culture; the proliferation and elongation medium is MS medium adding 2.28 to 9.12 μM ZT; step 3, transferring to the rooting medium for rooting induction to obtain rooted For seedlings, the rooting medium is 1 / 2MS medium added with 2.07-8.29 μM IBA; step 4, transplanting the obtained rooted seedlings into a cultivation medium for cultivation; the present invention uses the stems of Ilex serrata seedlings as explanted Through the proliferation and cultivation of seedling stems with plant growth regulators and the induction of adventitious roots, the micropropagation and rapid propagation system of sterile seedlings has been successfully obtained. The rooting rate of Ilex serrata tissue culture seedlings is as high as 90%, and the transplanting survival The rate is as high as about 90%.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a method for tissue culture and rapid propagation of Ilex serrata using stem segments as explants. Background technique [0002] Ilex serrata is a multi-branched evergreen shrub, up to 5 meters high; the leaves are leathery, obovate, elliptic or oblong-elliptic, 1-7 male flowers are arranged in cymes, 4 bases, white; calyx disc-shaped , petals 4, broadly elliptic, corolla diameter about 6 mm, petals ovate, ovary ovoid, fruit spherical, black when mature; endocarp leathery. The flowering period is from May to June, and the fruiting period is from August to October. Born in hills, mountain forests or bushes at an altitude of 700-2100 meters. Distributed in China, Japan and Korea. It is often cultivated as an ornamental tree species in gardens, and it is also cultivated in Europe and America. The fruit of Ilex serrata belongs to the seeds of plants, and it is very difficu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 杨玉洁郝明灼邹义萍耿芳
Owner YANGTZE UNIVERSITY
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