54 results about "Dihydrocapsaicin" patented technology

The invention relates to the field of separation and purification of capsaicin and paprika red pigment, and particularly discloses a method for preparing paprika red pigment and a capsaicin material. According to the method, capsicum oleoresin is subjected to three-level molecular distillation to respectively obtain the capsaicin material and the paprika red pigment, wherein the total content of capsaicin and dihydrocapsaicin in the capsaicin material is greater than 70%, and the color value of the paprika red pigment is higher than 200. The capsaicin material is recrystallized and purified, the total content of capsaicin and dihydrocapsaicin in crystallization is more than 85%, further capsaicin crystals and dihydrocapsaicin crystals are obtained through preparation and separation of high performance liquid chromatography, and the content of each of the capsaicin crystals and the dihydrocapsaicin crystals is more than 95%. The method is simple and convenient to operate and low in production cost, has a good separation effect and is suitable for industrial production; and the content of the products is high.

The invention discloses a halogen-free flame retardant cable material which is prepared from the following raw materials in parts by weight: 60-80 parts of LDPE (Low-Density Polyethylene) resin, 15-25 parts of EPDM (Ethylene-Propylene-Diene Monomer) resin, 5-15 parts of EVA (Ethylene Vinyl-Acetate Copolymer) resin, 70-90 parts of aluminium hydroxide, 6-8 parts of red phosphorus, 25-30 parts of magnesium hydroxide, 2-3 parts of hydrotalcite, 5-10 parts of maleic anhydride grafting, 1-2 parts of antioxidant and 1-1.5 parts of silicon-copper powder. The invention aims at overcoming the defects in the prior art; the magnesium hydroxide as a flame retardant is added in the cable material, so the flame-retardant effect is achieved; by adding abamectin, asarinin and dihydrocapsaicin, the condition that the cable is bitten by termite can be effectively prevented; the silicon-copper powder as a lubricating agent, the maleic anhydride grafting as a compatilizer, the antioxidant and the other components are also added, so that various properties of the flame-retardant material are improved, and the service life of the flame-retardant material is prolonged.

The invention relates to a preparation method of an active ingredient in chili and particularly relates to a production process of capsaicin. According to the production process disclosed by the invention, a supercritical fluid extraction method is adopted for separating capsaicin and capsanthin, and a high performance liquid chromatography is further used for performing preparation on a crude extract to obtain high-purity nordi-hydrocapsaicin, capsaicin and dihydrocapsaicin. The production process disclosed by the invention has the advantages of quickness, high efficient and energy conservation and can be industrially popularized.

The invention discloses a method for measuring capsaicin matters in hot peppers and hot pepper products. The method comprises the following steps of: (1) respectively diluting a capsaicin standard substance and a dihydrocapsaicin standard matter and measuring standard peak areas of different concentrations by using a liquid-phase analysis method; (2) preparing a sample reagent from the hot peppers or hot pepper products according to a well-known method, namely a liquid-phase measuring method; (3) carrying out liquid-phase analysis; (4) calculating the capsaicin matters; and (5) calculating the Scoville heat scale and the like. The method for measuring the capsaicin matters in the hot peppers and hot pepper products, disclosed by the invention, has the positive meaning that the capsaicin matters of the hot peppers and the hot pepper products can be conveniently, rapidly and accurately measured, and the process of making a standard curve in each time can be saved.

The invention relates to a colloidal gold immunochromatography test strip for rapidly detecting capsaicinoids as well as a preparation method and application thereof. The colloidal gold immunochromatography test strip is characterized by comprising a paperboard, wherein a water absorption pad, a detection pad, a gold mark pad and a sample pad are stuck on one face of the paperboard in sequence from top to bottom; the adjacent pads are overlapped and connected at a connection part; the detection pad is used as a base pad of a nitrocellulose membrane; the nitrocellulose membrane is provided with a transverse quality control line and a detection line from top to bottom; a rabbit anti-mouse polyclonal antibody covers the quality control line; a capsaicinoids coating antigen coats the detection line; the gold mark pad is transversely sprayed with a nano gold marked common monoclonal antibody resisting capsaicin, dihydrocapsaicin and synthesized capsaicin; and the nano gold marked common monoclonal antibody resisting capsaicin, dihydrocapsaicin and synthesized capsaicin are produced by secretion of hybridoma cell strains YQQD8 with the preservation number of CCTCC (China Center For Type Culture Collection) NO.C201534. The colloidal gold immunochromatography test strip for rapidly detecting the capsaicinoids, provided by the invention, can be used for simultaneously detecting the total content of the capsaicin, the dihydrocapsaicin and the synthesized capsaicin; and the detection sensitivity is high and the minimum detection limit is 10ng/mL.

The invention relates to a time-resolved fluorescence immunochromatographic test strip for quantitative detection of capsaicinoids and a preparing method and application thereof. The time-resolved fluorescence immunochromatographic test strip comprises a fluorescence test strip, europium-labeled anti-capsaicine, dihydrocapsaicin, olepresin capsaicin universal monoclonal antibody reagent and a reaction flask, wherein the fluorescence test strip comprises a paperboard, a water absorption mat, a detection mat and a sample mat adhere to the viscous surface of the paperboard from top to bottom in sequence, the adjacent mats are connected in an overlapping mode, a nitrocellulose membrane serves as the base mat of the detection mat, a horizontal quality control line and a detection line are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the detection line is coated with a capsaicinoids envelope antigen, and anti-capsaicine, dihydrocapsaicin, olepresin capsaicin universal monoclonal antibody are generated through secretion of a hybridoma cell strain with the preservation number of CCTCC NO.C201534. The test strip can be used for quantitative detection of the content of capsaicinoids, operation is easy and quick, and accuracy is high.

The invention relates to a hybridoma cell line YQQD8 and general monoclonal antibodies produced by the same to capsaicine, dihydrocapsaicin and nonivamide. With the collection number CCTCC NO.C201534, the cell line YQQD8 has been collected in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan city of China on April 23th, 2015 and is classified and named as the hybridoma cell line YQQD8. Titer of mouse hydroperitoneum antibody of YQQD8, tested by a conventional non-competitive enzyme linked immunosorbent assay (ELISA) method, can be up to 2.56*106. The general monoclonal antibodies to capsaicine, dihydrocapsaicin and nonivamide, produced by the YQQD8, can be applied to identify capsaicine, dihydrocapsaicin and nonivamide effectively; 50% inhibition concentrations (IC50) of the YQQD8 to capsaicine, dihydrocapsaicin and nonivamide are sequentially 8.5ng/mL, 5.0ng/mL and 13.5ng/mL respectively; and cross reactivity to capsaicine, dihydrocapsaicin and nonivamide ranges from 62.9% to 170%. The hybridoma cell line YQQD8 can be applied to quantitative measurement of total content of capsaicine, dihydrocapsaicin and nonivamide.

The invention belongs to the field of artificial synthesis of natural products and relates to an artificial synthesis method of a capsaicin homologue. The artificial synthesis method of the capsaicin homologue is to lead 4-hydroxy-3-methoxy-benzylamine hydrochloride to be reacted with the corresponding long-chain acid (E)-8-methyl-6-nonenoic acid and 8-methyl nonanoic acid or 7-methyl octanoic acid for preparing the capsaicin homologue. The long-chain acid synthesis method is characterized in that the method can avoid the use of triphenyl phosphine and other toxic substances which are commonly used in the existing patents and toxic substance-triphenyl phosphineoxide in byproducts, thereby being relatively environment-friendly. For different capsaicin homologues, the required starting raw materials are different. In particular, dihydrocapsaicin is applicable to large-scale industrial production.

A preparation method of high purity capsaicin monomer comprises the following steps: using alcohol to extract capsaicin from pepper powder or pepper essential oil, then using macroporous resin to enrich the extract so as to obtain pepper total alkaloid; adopting a high speed counter-current chromatography method to separate the pepper total alkaloid, wherein the separating solvent system is composed of alkane, ester, alcohol, and water, and acid and alkali can be added too; adding the solvent system into a separating funnel, fully vibrating the separating funnel, allowing the separating funnel to stand still so as to divide the solution to different layers, collecting the upper-layer solvent as the stationary phase and the lower-layer solvent as the mobile phase; dissolving a sample in a mixed solvent of the upper-layer solvent and the lower-layer solvent, injecting the sample into a detector, separating, and collecting the target components according to the spectrogram on the detector so as to obtain the capsaicin monomer and dihydrocapsaicin monomer. The method has the advantages of simple technology process, low cost, high product purity, high yield, and environment-friendliness, can rapidly prepare capsaicin monomer and dihydrocapsaicin monomer with a content of more than 98%, and is suitable for industrial production of high purity capsaicin and dihydrocapsaicin.

The invention provides a method for quantitatively detecting dihydrocapsaicin. The method comprises the following steps of: acquiring optical data of the dihydrocapsaicin in a sample by using a near-infrared spectrometer; correlating the optical data with content data of the dihydrocapsaicin in the sample detected by a chemical analysis method; establishing a calibration model by a partial least squares method; and substituting the optical data of the dihydrocapsaicin in a sample to be detected into the model to acquire the content of the dihydrocapsaicin in the sample to be detected. A dihydrocapsaicin quantitative analysis model established by the method has high accuracy, and can accurately and reliably predict the content of the dihydrocapsaicin in an actual sample. The content of the dihydrocapsaicin measured by a near-infrared spectrometer method and a result measured by the chemical analysis method do not have obvious differences; and by the method, the content of the dihydrocapsaicin can be detected nondestructively.

The invention relates to a method for the low-pressure silica gel column chromatographic separation of capsaicin and dihydrocapsaicin. Capsaicin and dihydrocapsaicin can be obtained by the following main procedures: using capsaicinoids as a raw material, carrying out low-pressure silica gel column chromatographic separation, eluting with petroleum ether and other solvents, carrying out HPLC (high performance liquid chromatography) analysis and the like. Compared with the traditional method, the invention has the advantages of less environmental pollution, high product purity, low cost and the like, thereby being applicable to industrial production.

The invention discloses a method of separating and purifying capsaicin and dihydrocapsaicin from pepper fruits. The method comprises the following steps: performing supercritical carbon dioxide extraction of pepper fruits; performing reduced-pressure concentration to obtain capsicum oleoresin; performing methanol solution extraction and alkali extraction and acid deposition of the capsicum oleoresin to obtain a capsaicin substance crystal rich in capsaicin and dihydrocapsaicin; with n-hexane-ethyl acetate-methanol-water as a solvent system and high-speed counter-current chromatography (HSCCC)for preparation, monitoring the purity by high performance liquid chromatography; according to the HSCCC as well as the eluent with relatively high purity, performing reduced-pressure concentration and vacuum drying to obtain capsaicin with purity higher than 97.0% and dihydrocapsaicin with purity higher than 96.0%, wherein the extraction efficiencies of the capsaicin and the dihydrocapsaicin areboth higher than 93%, and the recovery rates are both higher than 70%. The method disclosed by the invention makes full use of the pepper seeds, has the advantages of easiness in operation, low cost,high repeatability, high efficiency and high product purity, and can be applied to large-scale production.

The invention discloses a Hangzhou-pepper capsaicin concentration extraction determination method, which comprises: carrying out vacuum freeze drying on fresh hot pepper, grinding into powder, extracting with a methanol-tetrahydrofuran solution, and concentrating the supernatant obtained through the extracting to obtain a concentrated solution; analyzing a capsaicin standard substance and a dihydrocapsaicin standard substance by using a high performance liquid chromatography-ultraviolet detector to obtain the corresponding relationship between the capsaicin concentration and the chromatographic peak area and the relationship between the dihydrocapsaicin concentration and the chromatographic peak area; and analyzing the concentrated solution by using the high performance liquid chromatography-ultraviolet detector under the same analyzing conditions so as to finally obtain the capsaicin concentration and the dihydrocapsaicin concentration in the sample to be determined. According to thepresent invention, by using the freeze drying technology, the water in the fresh hot pepper is removed while the active substances are retained to the maximum extent, such that the capsaicin loss is less compared to other drying methods; and with the method, the problem that the fresh hot pepper cannot be stored for a long time during the large-scale determination analysis can be solved.

The invention discloses environment-friendly paint for preventing moisture and expelling parasites of furniture and a preparation method thereof. The environment-friendly paint is prepared by mixing acrylic emulsion, propylene glycol monomethyl acetate, epoxy resin, polyvinyl alcohol, ethyl cellulose, mica powder, ethylene glycol, tung oil, water, cobalt oxide, titanium dioxide and parasite-expelling liquid, wherein the parasite-expelling liquid is prepared from szechwan Chinaberry fruit, wormwood, eucalyptus, geranium, dihydrocapsaicin, menthol and alcohol. The environment-friendly paint has the advantages that the effective parasite-killing matter of the traditional Chinese medicine is extracted by the alcohol, is mixed with the parasite-expelling matter, and then is mixed with the poison-free matter to prepare the parasite-expelling paint; the poison, harm and any side effect are avoided, the parasite-expelling effect is obvious, the efficiency is high, the poison is avoided, the use value is high, and the environment-friendly paint is suitable for being popularized and used.

The invention discloses an anti-white ant paint for furniture. The paint is prepared from the following raw materials in parts by weight: 30-35 parts of ethyl acetate, 12-15 parts of titanium dioxide, 7.5-8.5 parts of polytetramethylene ether glycol, 0.2-0.6 part of aluminum stearate, 0.4-1.5 parts of a drier, 3-5 parts of xylene, 25-30 parts of chlorine-containing alkyd resin, 2-4 parts of a dispersant, 1.5-2.5 parts of a flatting agent, 4-10 parts of methylbenzene, 0.5-0.9 part of organic silicone oil, 7-9 parts of a nano aluminum oxide sol, 2.5-4 parts of phosphoric acid, 1.6-2.5 parts of ethylene glycol, 1.4-4.5 parts of wollastonite powder, 30-35 parts of water and a proper amount of pigments. According to the invention, auxiliary materials, namely abamectin and dihydrocapsaicin are used as an anti-white ant agent, so that the formula of the paint is reasonable in proportions. The paint does not fall off and has no cracks, and most raw materials are organic raw materials which are green, energy-saving, safe and harmless to human body.

The invention relates to a preparation method of hot pepper seasoning. The preparation method of the hot pepper seasoning comprises the specific preserving steps of pretreating hot peppers, drying the pretreated hot peppers, cutting the dried hot peppers into hot pepper segments, performing vacuum pressure treatment, immersing the hot peppers after being subjected to vacuum pressure treatment, performing secondary vacuum pressure treatment, performing crushing and performing mixing. The hot peppers are pretreated, so that the volatilizing amount of capsicine and the volatilizing amount of dihydrocapsaicin in the red pepper powder are reduced, the content of the capsicine and the content of the dihydrocapsaicin in the hot pepper powder are increased, and waste of a great quantity of raw materials is reduced.

The invention discloses a method for raising the proportion of capsaicin monomer in capsicum oleoresin. The method comprises the steps of dissolving the capsicum oleoresin obtained through organic solvent extraction and decompressed concentration with an alcoholic solution, injecting the capsicum oleoresin into a chromatographic column filled with macroporous adsorption resin, leaving the capsicum oleoresin standing still for being adsorbed for 2-4 hours, then using an alcoholic solution for elution, conducting decompressed concentration on an elution solution, and obtaining capsicum oleoresin free of peculiar smell, wherein the total capsaicin content is 90-91%, and the proportion of the capsaicin monomers reaches 80-85%; continuing with elution with the alcoholic solution till eluant contains no capsaicin, conducting decompressed concentration on the eluant, and obtaining capsicum oleoresin free of peculiar smell, wherein the total capsaicin content is 93-94%, and the proportion of dihydrocapsaicin monomer reaches 45-50%. The method is obvious in effect, simple in process and easy and convenient to operate for separating capsaicin and dihydrocapsaicin, the solvent and resin can be recycled, no emission of industrial wastewater, waste gases and residues is generated, and the method is suitable for large-scale industrialized production.

The invention discloses a biopesticide capable of being directly sprayed to plants. The biopesticide comprises the following substances in parts by weight: 40-45 parts of hirsutella thompasonii fisher preparations, 15-20 parts of brassinolide, 5-13 parts of aconitine, 20-30 parts of polyvinyl alcohol, 5-19 parts of azadirachtin, 0.5-10 parts of celastrus angulatus, 3-13 parts of oilseed straw powder, 5.5-8.4 parts of dihydrocapsaicin, 5-11 parts of ammonium dihydrogen phosphate, and 11-16 parts of sodium polyphosphate. The biopesticide is fast produced and has good stability and effect, and the method is simple and convenient; the biopesticide is directly sprayed onto leaves and can be naturally eaten by insects, the operation is simple, the pesticide application mode in the truly meaning can be realized, and the biopesticide can be widely popularized.

The invention discloses a serratia marcescens immunoenhancer for frog immunization. The immunoenhancer comprises serratia marcescens agent, vitamin C, anthocyanin, citric acid, dihydrocapsaicin and glycerin, the serratia marcescens agent serves as a main ingredient and plays a role in enhancing frog immunity, and anthocyanin and dihydrocapsaicin are synergistic, so that immune effect can be improved.

The invention discloses a method for preparing rice straw feed. After being diluted by water, cellulase and lactobacillus series are mixed proportionally, then sprayed to the surface of straw and turned and mixed to be uniform; after the sectioned straw is compacted, a plastic film coats the sectioned straw so that the straw and air can be isolated, and after sealed storage for 90 d, the rice straw feed can be obtained. The method is simple and applicable and low in cost; on the basis, a small amount of dihydrocapsaicin and tricin is added, the sheep immunity can be effectively improved, and the weight gaining effect is obvious.