11 results about "Dihydrocapsaicin" patented technology

The invention discloses a method for synthesizing dihydrocapsaicin, and the method comprises the following steps: synthesizing a first intermediate 1-isobutylcyclohexene, a second intermediate 8-methyl-6-oxonanoic acid, a third intermediate 8-methylnonanoic acid and a target product dihydrocapsaicin. The method uses cyclohexanone, isobutylmagnesium bromide and vanillin ammonium hydrochloride as raw materials, and obtains the product dihydrocapsaicin by stepwise reaction. Each reaction step adopts a conventional chemical reaction, and the reaction conditions are mild. The feasibility of the synthesis process is high, the safety and environmental protection are strong, and the product has a high yield and good purity, and is suitable for industrial production.

The invention provides a silica gel foam sealing material capable of preventing termite gnawing and a preparation method of the silica gel foam sealing material, and belongs to the technical field of polymer sealing materials. The raw material formula of the silica gel foam sealing material is as follows: vinyl silicone oil, a reinforcing filler, a flame-retardant filler, a contact insecticide, a bait, an odorant, a structure control agent, an inhibitor, a platinum catalyst, a foaming agent, hydroxyl silicone oil and hydrogen-containing silicone oil. Wherein the contact insecticide is one or more of pyrethroids, neonicotinoids, phenyl pyrazoles, boric acid, glycine, erythritol and fipronil; the bait agent is one or more of diatomite, talcum powder, capsaicin, dihydrocapsaicin and denatonium benzoate; the odorant is one or more of camphor oil, peppermint oil, limonin, limonene, citral, capraldehyde, octanal and nonanal. The silica gel foam sealing material disclosed by the invention has excellent flame retardant property, mechanical property and ant gnawing resistance, and the integrity of the sealing material can be well protected.

The application provides an anti-termite sealing material of silica gel foam and a preparation method thereof, and belongs to the technical field of polymer sealing materials. The raw material formula of the anti-termite sealing material of silica gel foam is as follows: vinyl silicone oil, reinforcing filler, flame-retardant filler, contact killing agent, bait agent, odor agent, structure control agent, inhibitor, platinum catalyst, foaming agent, hydroxyl silicone oil and hydrogen-containing silicone oil; wherein the contact killing agent is one or more of pyrethroid, neonicotinoid, phenylpyrazole, boric acid, glycine, erythritol and fipronil; the bait agent is one or more of diatomite, talcum powder, capsaicin, dihydrocapsaicin and denatonium benzoate; and the odor agent is one or more of camphor oil, peppermint oil, limonin, limonene, citral, decanal, octanal and nonanal. The anti-termite sealing material of silica gel foam has excellent flame-retardant performance, mechanical performance and anti-termite biting performance, and can well protect the integrity of the sealing material.

The application provides a method for inducing capsaicin synthesis by using a light signal transcription factor CaBBX2, and specifically comprises an experimental method for studying the influence of silencing CaBBX2 gene on capsaicin synthesis in a living plant. The CaBBX2 gene is silenced in a living pepper, and the contents of capsaicin, dihydrocapsaicin and total capsaicin and the expression changes of capsaicin synthesis related genes in the pepper fruits at 16d, 25d, 33d and 38d after pollination are observed and analyzed. The experimental results show that the content of capsaicin is significantly reduced compared with the empty control group after the CaBBX2 gene is silenced, which indicates that CaBBX2 is related to the biosynthesis of capsaicin, and the expression of capsaicin synthesis related genes is also reduced to different degrees with the silencing of CaBBX2, further verifying that CaBBX2 can affect the synthesis of capsaicin by regulating the expression of capsaicin synthesis related genes.

The invention discloses an application of dihydrocapsaicin in treatment of vascular calcification. Specifically, the invention discloses application of dihydrocapsaicin or pharmaceutically acceptable salt thereof, the dihydrocapsaicin or the pharmaceutically acceptable salt thereof is used for preparing a preparation or a composition, and the preparation or the composition is used for treating vascular calcification. Particularly, calcification of vascular smooth muscle cells can be effectively treated.

The invention relates to a high-purity capsaicin composition and a preparation method and application thereof, the total content of capsaicin and dihydrocapsaicin in the composition is greater than or equal to 98.5%, the balance is impurities, the impurities comprise cis-capsaicin, the content of the cis-capsaicin in the composition is less than or equal to 1.0%, and the mass ratio of capsaicin to dihydrocapsaicin in the composition is (1.5-2.5): 1; when capsaicin and dihydrocapsaicin are sequentially prepared, the crystallization mother liquor and the eluent are recycled, the utilization efficiency of materials is improved, and the method has the advantages of being easy and convenient to operate, better in cost, capable of reducing emission of three wastes, suitable for industrial production and the like; the high-purity capsaicin composition has the advantages of high purity, stable components, natural taste, good thermal stability and the like, and can be used as a food additive.

The invention discloses application of an ERF / AP2 family transcription factor CcADAP in regulation and control of pungency degree of Chinese pepper. A silent material of ADAP is obtained through a gene silencing technology. The content of capsaicin and dihydrocapsaicin in silent plant fruits is determined, it is found that the content of capsaicin and dihydrocapsaicin can be effectively reduced through silent expression of the transcription factor ADAP in capsicum, and a key target gene is provided for development of a new variety of capsicum with low pungency degree and flavor and development of a fresh food market.

The application belongs to the technical field of plant genetic engineering, and discloses a kind of pepper CaERF13 gene and its coding protein in the application of capsaicin synthesis regulation, the nucleotide sequence of the pepper CaERF13 gene is as shown in SEQ ID NO:1, the amino acid sequence of its coding protein is as shown in SEQ ID NO:2.The application identifies and verifies the specific positive regulation function of CaERF13 gene in capsaicin biosynthesis for the first time.It is directly proved by virus-induced gene silencing technology that down-regulation of the expression of CaERF13 can lead to a significant decrease in the accumulation of capsaicin and dihydrocapsaicin in the placenta of pepper fruits, and the expression level of multiple capsaicin synthesis pathway genes is down-regulated.

The invention discloses a synthesis method of dihydrocapsaicin, which comprises the following steps: taking terminal halogenated fatty alcohol as an initial raw material, and protecting hydroxyl of the terminal halogenated fatty alcohol to obtain a hydroxyl-protected intermediate; carrying out coupling reaction on the hydroxyl protected intermediate and a Grignard reagent derived from halogenated hydrocarbon under the catalysis of a copper-based Lewis acid catalyst; or carrying out coupling reaction on the Grignard reagent derived from the hydroxyl protected intermediate and halogenated hydrocarbon under the catalysis of a copper-based Lewis acid catalyst to obtain 8-methyl nonanol, oxidizing the 8-methylnonanol into 8-methylnonanoic acid; the method comprises the following steps: reacting 8-methylnonanoic acid with an activating agent, and then reacting with vanillin amine to obtain dihydrocapsaicin. According to the present invention, the cheap and easily available bulk chemical product terminal halogenated fatty alcohol is adopted as the raw material, the high yield and high purity synthesis of the dihydrocapsaicin is successfully achieved through the conventional chemical reaction with characteristics of mild condition and simple operation, and the whole process has characteristics of strong practicality, low production cost and significant market competition advantage.

The invention discloses a synthesis method of 8-methylnonanoic acid, which takes halogenated isobutane and 1-bromo-6-chlorohexane as raw materials, and comprises the following steps: converting the halogenated isobutane into an organic metal reagent, and coupling the organic metal reagent with the 1-bromo-6-chlorohexane under the catalysis of transition metal salt to obtain chlorinated isodecane; and oxidizing chloromethyl at the tail end of the chloro-isodecane into carboxyl to obtain 8-methylnonanoic acid. The reaction activity difference of bromine and chlorine is utilized, a target carbon skeleton is constructed in one step through direct selective coupling, terminal oxidizable sites are reserved at the same time, active functional groups such as carboxyl do not need to be protected and deprotected, synthesis steps are simplified, and harsh reaction conditions and use of expensive reagents are avoided. The method is safe, simple and convenient to operate, the obtained 8-methylnonanoic acid is high in purity and ideal in yield, and an economic, green and efficient intermediate synthesis scheme is provided for industrial production of dihydrocapsaicin.

The present invention discloses a universal monoclonal antibody HOPE1 against natural capsaicin, dihydrocapsaicin, and synthetic capsaicin, and its application. The heavy chain variable region sequence of the antibody is shown in SEQ ID NO. 1, and the light chain variable region sequence is shown in SEQ ID NO. 2. The monoclonal antibody HOPE1 has a half-inhibitory concentration of 0.32 ng / mL for natural capsaicin, 0.07 ng / mL for synthetic capsaicin, 0.21 ng / mL for dihydrocapsaicin, and a mixture of natural capsaicin, synthetic capsaicin, and dihydrocapsaicin, respectively, and has a strong affinity for capsaicinoids. The IC-ELISA immunoassay method and time-resolved fluorescence immunochromatography assay for detecting the content of capsaicinoids established using the monoclonal antibody HOPE1 provided by the present invention have the advantages of high sensitivity and low detection limit.