Preparation method of high purity capsaicin monomer

Abstract

A preparation method of high purity capsaicin monomer comprises the following steps: using alcohol to extract capsaicin from pepper powder or pepper essential oil, then using macroporous resin to enrich the extract so as to obtain pepper total alkaloid; adopting a high speed counter-current chromatography method to separate the pepper total alkaloid, wherein the separating solvent system is composed of alkane, ester, alcohol, and water, and acid and alkali can be added too; adding the solvent system into a separating funnel, fully vibrating the separating funnel, allowing the separating funnel to stand still so as to divide the solution to different layers, collecting the upper-layer solvent as the stationary phase and the lower-layer solvent as the mobile phase; dissolving a sample in a mixed solvent of the upper-layer solvent and the lower-layer solvent, injecting the sample into a detector, separating, and collecting the target components according to the spectrogram on the detector so as to obtain the capsaicin monomer and dihydrocapsaicin monomer. The method has the advantages of simple technology process, low cost, high product purity, high yield, and environment-friendliness, can rapidly prepare capsaicin monomer and dihydrocapsaicin monomer with a content of more than 98%, and is suitable for industrial production of high purity capsaicin and dihydrocapsaicin.

Description

technical field [0001] The invention belongs to the field of natural product extraction, separation and purification, in particular to a method for purification of natural active ingredients, more specifically to a method for separating and purifying capsaicin and dihydrocapsaicin monomers from total alkaloids of capsicum by using high-speed countercurrent chromatography Methods. Background technique [0002] Capsaicin (also known as capsaicin, Capsaicinoids) is a class of extremely pungent vanilla amide alkaloids, and is the main spicy component in capsicum fruit, wherein capsaicin and dihydrocapsaicin account for more than 90% of the total alkaloids. At present, capsaicin is mainly used as an analgesic for external use, an analgesic for detoxification, an antipruritic, a stomach-invigorating and digestive agent, a drug for preventing heart disease, a weight-loss drug, an insecticide, and military applications. Capsaicin has complex physiological and pharmacological activi...

AI Technical Summary

Problems solved by technology

Patent CN102557981A uses high-speed countercurrent chromatography to separate, but only obtains capsaicin with a purity of 90.3% to 92.8%, which is relatively low in purity; patent CN101200433B uses high-speed countercurrent chromatography to obtain capsaicin, dihydrocapsaicin and nordihydrocapsaicin , but the solvent used contains halogenated alkanes, which is highly toxic; the flow rate of the above two high-speed countercurrent chromatography separation methods is only 2.0mL / min, which takes a long time, consumes a lot of solvent, and is costly, and is not suitable for industrial production.

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