Biopesticide capable of being directly sprayed to plants

Abstract

The invention discloses a biopesticide capable of being directly sprayed to plants. The biopesticide comprises the following substances in parts by weight: 40-45 parts of hirsutella thompasonii fisher preparations, 15-20 parts of brassinolide, 5-13 parts of aconitine, 20-30 parts of polyvinyl alcohol, 5-19 parts of azadirachtin, 0.5-10 parts of celastrus angulatus, 3-13 parts of oilseed straw powder, 5.5-8.4 parts of dihydrocapsaicin, 5-11 parts of ammonium dihydrogen phosphate, and 11-16 parts of sodium polyphosphate. The biopesticide is fast produced and has good stability and effect, and the method is simple and convenient; the biopesticide is directly sprayed onto leaves and can be naturally eaten by insects, the operation is simple, the pesticide application mode in the truly meaning can be realized, and the biopesticide can be widely popularized.

Description

technical field [0001] The invention relates to a biological pesticide which can be directly sprayed on plants. Background technique [0002] RNA interference (RNA interference, RNAi) refers to the phenomenon that exogenous or endogenous double-stranded RNA (dsRNA) specifically induces gene expression silencing. It is a gene regulation mechanism that is highly conserved in evolution and ubiquitous in the biological world. . First discovered in 1998 in the nematode Caenorhabditis elegans, the phenomenon has since been found in fungi, plants, insects and animals. The mechanism of RNAi is: the long-chain dsRNA introduced in vitro or generated by endogenous transcription is cut into 21-25nt (base) siRNA by Dicer family RNase-III, and the siRNA is further combined with Argonaute protein to form RISC (RNA-induced silencing complex), and finally RISC-mediated siRNA antisense strand and target mRNA molecule complementary combination causes specific degradation of homologous target...

AI Technical Summary

Problems solved by technology

At present, the major commonality and key technologies that restrict the use of RNAi technology to produce new biopesticides are: 1. The existing technical systems and strategies that use transgenic technology to transfer crops to control pests have food safety problems for transgenic plants; 2. Engineering strains expressing dsRNA The effect is slow, the control effect is low, and the environmental adaptability is difficult to evaluate; 3. The cost of in vitro transcription and synthesis of dsRNA is high, and the stability is not good, etc.

These problems greatly limit the wide application of this technology in the field of pest control.