35 results about "Tricin" patented technology

The invention relates to a preparation method of tricin. The method comprises the following steps of: crushing lucerne serving as a raw material; adding the crushed lucerne into an extraction kettle; collecting a tricin extract by performing hypercritical CO2 extraction; dissolving with ethanol; adding polyamide resin for sample stirring; vaporizing a solvent; loading on a column; washing impurities away with water; eluting with acid alcohol; collecting an eluent; concentrating to a small volume; standing for crystallizing; and filtering crystals out and recrystallizing with ethyl acetate to obtain a tricin product. The method has the advantages of low energy consumption, short preparation period and high product purity.

The invention relates to a full alfalfa peptide nutriment and a preparation method of the full alfalfa peptide nutriment. The full alfalfa peptide nutriment provided by the invention contains 3 percent to 90 percent of a mixed active peptide of oligopeptide, short peptide and polypeptide, and further contains linoleic acid, linolenic acid, oleic acid, carotene, VB1, VB2, VB12, VC, VK, VA, nicotinic acid, calcium, phosphorus, iron, potassium, magnesium, sodium, zinc, selenium, manganese, tricin, medicagol, glucose and water; the full alfalfa peptide nutriment is prepared by the following steps: crushing alfalfa into powder and adding water to prepare paste; grinding the paste and carrying out ultrahigh-pressure homogenization to enable the material fineness to reach 700 meshes to 800 meshes; carrying out pulse type steam impacting under high pressure and high temperature; adding amylase and lipase, and standing and carrying out constant-temperature biochemical treatment; adjusting the pH (Potential of Hydrogen) value to be 6 to 8; adding neutral proteinase and homogenizing in an ultrahigh-pressure homogenization machine; standing and carrying out biochemical treatment again; finally, killing enzyme and sterilizing, and separating out a full alfalfa peptide raw solution, so as to prepare the full alfalfa peptide nutriment.

The invention belongs to the technical field of biology, and discloses a method for extracting tricin from alfalfa. The method is characterized in comprising the steps that: (1) alfalfa is crushed; 5-8 time amount of 60-80% ethanol is added for carrying out reflux extraction 2-3 times; an obtained extraction liquid is concentrated until no alcohol is left; the extraction liquid is absorbed by using polyamide resin column; an ethanol solution is used in gradient elution; thin-layer detection is carried out; high-concentration fraction is collected and concentrated; ethyl acetate is added for extracting; and ethyl acetate is recovered, such that a crude product is obtained; (2) n-hexane, ethyl acetate, methanol, and water are mixed; an upper-phase is used for filling a high-speed countercurrent chromatographic column; a main machine is rotated, and a lower-phase is pumped in as a mobile phase; the crude product dissolved by the mobile phase is fed by using a feeding valve, and is subjected to UV detector online monitoring; a target component is collected; obtained fractions are combined; and the mixture is subjected to reduced-pressure drying, such that high-purity tricin is obtained. The method provided by the invention has the advantages of short-period, simple operation, large preparation amount, high yield, and the like. The method is suitable for preparations of high-purity tricin.

The invention relates to a pedicularis longiflora Rudolph.var.tubiformis formula granule, a preparation method and a detection method thereof. The formula granule is composed of pedicularis longifloraRudolph.var.tubiformis dry paste powder, silicon dioxide and sodium lauryl sulfate. The formula granule is prepared by performing digestion, spray drying and pelletizing on a single drug of the pedicularis longiflora Rudolph.var.tubiformis, and is convenient for a physician to carry out treatment based on syndrome differentiation and add and reduce according to syndromes; the detection method ofthe pedicularis longiflora Rudolph.var.tubiformis formula granule comprises performing thin layer chromatography identification by using a reference crude herb of the pedicularis longiflora Rudolph.var.tubiformis and a reference substance of luteolin, measuring the content of the main component luteolin by using high performance liquid chromatography, and detecting the content of tricin by using ultraviolet-visible spectrophotometry with the reference substance of the tricin as control; the detection method can reflect the quality of the pedicularis longiflora Rudolph.var.tubiformis formula granule comprehensively, guarantees complete reservation and stable content of the active ingredients of the pedicularis longiflora Rudolph.var.tubiformis formula granule, and makes the detection methodof the medicine quality more scientific and complete, and the medicine quality is better controlled.

The invention discloses a device for extracting quinoa flavonoids, which comprises a soaking tank, a water filter cylinder, a conveyor belt, an extraction tank, a conveying component and a crushing component, a supporting leg is arranged under the soaking tank, and a first feeding port is arranged above the soaking tank. There is a first outlet below the soaking tank, the height of the water filter cylinder is less than the height of the supporting legs, the water filter cylinder is located directly below the first discharge outlet, the conveyor belt is arranged between the water filter cylinder and the extraction tank, and the middle and upper part of the water filter cylinder is set There is a filter net, the transmission component is set in the water filter cylinder, the transmission component is located above the filter net, the side wall of the water filter cylinder is provided with a second discharge port, the second discharge port is located at the middle and lower part of one side of the transmission component, and the water filter cylinder corresponds to the outlet A protective door is hinged at the position of the material opening, and a transmission plate is provided on the outer wall of the water filter tube, the transmission plate is located below the discharge opening, and the other end of the transmission plate is located on the conveyor belt. The invention relates to the technical field of agricultural product processing, in particular to a quinoa flavonoid extraction device which can integrate quinoa soaking, crushing and flavonoid extraction.

The invention discloses a method for extracting tricin from corn bracts. The method includes the steps that the corn bracts are subjected to normal temperature extraction with water, and extraction residues are obtained; an ethanol solution with the volume fraction of 80% to 90% is used for conducting reflux extraction on the extraction residues, an acid medium is added to the ethanol solution, an obtained extracting solution is subjected to vacuum concentration, and concentrate is obtained; the concentrate is diluted with water, the pH value is adjusted to 7.8 to 8.2, and supernatant is taken after solid-liquid separation; macroreticular resin column chromatography is conducted on the supernatant, and corn bract extract containing tricin is obtained. Normal temperature extraction is firstly conducted with water during alcohol extraction, and therefore protein, polysaccharide and other impurities can be removed in advance. The acid medium is added to the ethanol solution used for reflux extraction, and therefore the extraction efficiency of low-polarity tricin can be effectively improved. The pH value of the concentrate diluent is adjusted to 7.8 to 8.2, at the moment, chlorophyll, protein, polysaccharide and other impurities in the concentrate diluent quickly settle in the weak base environment, and the extraction period of tricin is greatly shortened.

The invention discloses the application of Os07g0503900 protein in regulating the resistance of plants to weeds. The invention provides applications of Os07g0503900 protein or related biological materials in any of the following: regulating plant allelopathy; regulating plant resistance to weeds; regulating tricin-5-O-glucoside content in plants; Glycosylation generates tricin-5-O-glucoside; as or prepares glycosyltransferase; inhibits weed growth. Experiments show that Os07g0503900 can catalyze the glycosylation of tricin to generate tricin‑5‑O‑glucopyranoside, and tricin‑5‑O‑glucopyranoside can regulate plant allelopathy and inhibit the growth of weeds. It can be seen that Os07g0503900 has important application value in regulating the allelopathy of plants. The invention has important guiding significance for cultivating rice varieties with high allelopathic ability and regulating the biosynthesis of tricin-5-O-glucoside.

A buckwheat flavone sugar-free beverage, 1000mL of the beverage consists of 50-80mg buckwheat flavonoids or rutin, 5-15g xylitol, 0.2-0.6g sodium cyclamate, 5-10mg potassium carbonate, and 0.05-0.2 citric acid g. Prepared by adding purified water to 1000mL, replacing the above-mentioned sodium cyclamate with N-L-α-aspartyl-L-phenylalanine methyl ester, and the dosage is 0.05-0.15g. No sucrose, artificial essence, pigment and preservative are added to the beverage of the invention, the taste is pure, slightly sweet and refreshing, and it is a pure natural functional beverage. The invention is tested according to the national fruit and vegetable juice beverage hygiene standards, and the heavy metal content of arsenic, lead and copper conforms to the national fruit and vegetable juice beverage hygiene standards, and the total number of bacteria, coliforms, and pathogenic bacteria meet the national fruit and vegetable juice beverage hygiene standards. Standard, can be used as a drink, especially suitable for middle-aged and elderly people.

The invention discloses a quinoa flavone extraction device, which comprises a soaking tank, a water filtering cylinder, a conveying belt, an extraction tank, a conveying assembly and a smashing assembly, supporting legs are arranged below the soaking tank, a first feeding port is formed in the upper portion of the soaking tank, a first discharging port is formed in the lower portion of the soakingtank, and the height of the water filtering cylinder is smaller than that of the supporting legs; the water filtering cylinder is arranged right under the first discharging port, the conveying belt is arranged between the water filtering cylinder and the extraction tank, a filter screen is arranged at the middle upper part of the water filtering cylinder, the conveying assembly is arranged in thewater filtering cylinder and located above the filter screen, a second discharging port is formed in the side wall of the water filtering cylinder, and the second discharging port is located at the middle lower part of one side of the conveying assembly; a protective door is hinged to the position, corresponding to the discharging port, of the water filtering cylinder, a conveying plate is arranged on the outer side wall of the water filtering cylinder and located below the discharging port, and the other end of the conveying plate is arranged on the conveying belt. The invention relates to the technical field of agricultural product processing, in particular to a quinoa flavone extraction device capable of integrating quinoa soaking, crushing and flavone extraction.

The invention belongs to the technical field of drugs, food and health products, and discloses synergistic anti-tumor polyphenol composition and application. The polyphenol composition is the composition of two or more of tricin, quercetin, luteolin and p-coumaric acid, preferably, the composition of tricin and quercetin, the composition of tricin and luteolin, or the composition of p-coumaric acid and luteolin. The composition has obvious synergistic effect of resisting tumor cell proliferation, the effect is better than that of an independently used polyphenol compound, the dose of a drug can be reduced, and drug resistance of tumor cells is reduced. The polyphenol composition is used for preparing anti-tumor drugs, food, health products and functional food additives.

The invention discloses the application of triadone-5-O-glucoside in regulating the resistance of plants to weeds. The invention provides the application of tricin-5-O-glucoside in any one of the following: regulating plant allelopathy; regulating plant resistance to weeds; and inhibiting weed growth. Experiments have shown that tricin-5-O-glucoside can regulate the allelopathic effect of plants and can be used to inhibit the growth of weeds. The invention has important significance for the discovery of flavonoids in plant allelopathy and the development of environment-friendly green pesticides.

The invention discloses a method for simultaneously extracting tricin, eoixol and vanillic acid from imperata cylindrical by subcritical water, comprising the following steps: crushing an imperata cylindrical sample to obtain imperata cylindrical powder of 20-90 meshes; putting deionized water and the imperata cylindrical powder into a subcritical water extraction apparatus and extracting for 30-90 min so as to obtain an extracting solution; adding ethanol into the extracting solution to obtain mixed liquor, and centrifuging to obtain a supernatant and sediment; and carrying out reduced pressure distillation on the supernatant to obtain tricin, eoixol and vanillic acid products. The extraction solvent subcritical water is a typical green solvent, is nontoxic and harmless, and is low-cost and environment friendly. The active components tricin, eoixol and vanillic acid extracted from imperata cylindrical by the use of subcritical water have few impurity, are nontoxic and pollution-free, have no residue, and are low-cost and environment friendly. According to the method for simultaneously extracting tricin, eoixol and vanillic acid from imperata cylindrical by subcritical water, extraction efficiency is high, and other impurity components are easy to separate.

The invention provides alfalfa tea. The alfalfa tea comprises the following components: 80% of alfalfa leaves, 15% of white sugar, and 5% of jasmine flowers. The product has high nutrition value, andefficacies for cleaning spleen and stomach, benefiting large and small intestine, and discharging cystolith; alfafa contains abundant vitamin K, the content of the component is high and is higher thanthe content of other vegetables, and other vitamin C and B are abundant; Medicago sativa contains saponins, lucernol, sativol, daidzein, tricin, citrulline, beta-methyl-glucoside, protein, sugar andthe like; Medicago polymorpha contains carotene which is an essential element for human body; the product has sweet and light taste, slightly cold property, and can clear stomach heat, and the producthas effects of diuresis, dampness elimination, immunity improvement, and supplement of human body nutrition.

The invention discloses a high-efficiency biologically fermented feed. The biologically fermented feed comprises the following components: corn steep liquor, corn bran, bran, corn flour, soybean meal, lactic acid, mannan, yeast, Lactobacillus acidophilus, leeches Trichosanthin, amino acid trace elements, coumarin, turpentine, trichosanthin, buckwheat flavonoids. The feed formula of the present invention is reasonable, rich in raw materials, rich in trace elements, and all raw materials are pure natural green ingredients, balanced in nutrition, high in digestibility, low in cost, safe in product quality, and can effectively improve the growth quality of animals. Competitive in the market.