12 results about "Hypoproteinemia" patented technology

The invention belongs to the technical field of gene engineering, and discloses a cat serum albumin mutant as well as a CHO cell expression method and application thereof. The invention provides a cat serum albumin mutant Mut-FSA for the first time, and an optimized Mut-FSA sequence is finally formed by replacing the 558th K at the tail end of an FSA amino acid sequence with R. Compared with non-mutated FSA, the Mut-FSA can show more remarkable curative effect advantages in treatment intervention of cat hypoproteinemia or acute hemorrhage, in addition, the Mut-FSA not only can enable cats suffering from the hypoproteinemia to achieve rapid correction of serum albumin concentration within 24 hours after treatment intervention, but also can effectively improve the serum albumin level and overall prognosis of the cats. The recombinant FSA prepared by the invention not only can provide a more effective innovative therapy for cat hypoproteinemia or acute hemorrhage, but also has wide application prospect and industrialization value.

The application relates to the technical field of kidney dialysis medical equipment, in particular to a kidney dialysis blood multi-stage filtering device with optimized flow channels, which comprises a device body; three groups of filtering cylinders are driven in linkage by a micro motor, a rotating shaft, a driving gear and a transmission gear ring, the driving gear and the transmission gear ring are sequentially reduced from top to bottom, the three groups of filtering cylinders rotate at different rotating speeds, the internal filtering units are driven to work efficiently, the filtering unit apertures are sequentially reduced from top to bottom, the filtering unit apertures are respectively set as pretreatment filtering units, precision filtering units and deep adsorption filtering units, a grading purification system is formed, different molecular weight toxins can be targetedly removed, the linkage structure of a pawl, a ratchet ring, a connecting rotating rod and a return spring can dynamically adjust the angle of the filtering units according to the changes of blood flow and pollution degree, the occurrence risk of low proteinemia of patients can be effectively reduced, the device can be flexibly adapted to the disease differences of different patients, individualized precise treatment can be realized, and the kidney dialysis treatment effect is improved.

The traditional Chinese medicine composition disclosed by the invention adopts a method of warming yang and relieving exterior syndrome to achieve the effects of freeing lung and inducing diuresis, tonifying spleen and warming kidney, and strengthening defensive qi and diminishing swelling, and is prepared to be used for treating the immune-related glomerular diseases, especially membranous nephropathy and minimal lesion nephropathy. The traditional Chinese medicine composition is a pure traditional Chinese medicine preparation, clinical observation and in-vivo and in-vitro basic tests show that the prescription can remarkably improve edema, massive proteinuria, hypoproteinemia and the like, meanwhile, compared with the prior art, the traditional Chinese medicine composition has a better effect on symptoms such as stomachache and diarrhea, has an anti-inflammatory effect and does not have obvious side effects in clinical application. The traditional Chinese medicine composition has an excellent effect on patients suffering from membranous nephropathy and minimal lesion nephropathy caused by syndrome differentiation of spleen-kidney yang deficiency, internal rheumatism and water-fluid overflowing.

The invention provides cat serum albumin as well as a separation method and application thereof, and relates to the technical field of genetic engineering. According to the invention, mutation is carried out on the cat serum albumin for the first time to obtain a beneficial mutation site, so that the cat serum albumin mutant which can be highly expressed in a pichia pastoris system is obtained. Moreover, the cat serum albumin mutant L94H disclosed by the invention not only has a good antioxidant effect, but also can remarkably improve the concentration of albumin in a cat body, and has a wide application prospect in the aspect of treating diseases such as cat hypoproteinemia, cirrhosis ascites and the like or relieving liver injury.

The invention belongs to the technical field of bioengineering, and particularly relates to a recombinant CSA / FSA protein as well as a preparation method and application thereof. The recombinant CSA / FSA protein is obtained by performing specific site mutation on an amino acid sequence of the dog / cat serum albumin, constructing a recombinant expression vector and expressing a recombinant host, the recombinant CSA / FSA protein is stable in source, relatively low in cost and high in safety, the performance is optimized through mutation, the half-life period is prolonged, and the recombinant CSA / FSA protein also has degradation resistance and can be used for preparing the recombinant CSA / FSA protein. Compared with natural serum albumin, the serum albumin provided by the invention has more excellent performance, and can better exert physiological functions of maintaining blood osmotic pressure, transporting nutrient substances, detoxifying, regulating immunity and the like in dogs and cats. The traditional Chinese medicine composition has important clinical application value and market prospect in the application of treating shock, hypoproteinemia and related diseases caused by factors such as blood loss or burn.

The present invention belongs to the technical field of genetic engineering, and discloses a feline serum albumin mutant, its expression method in CHO cells and its application. The present invention for the first time provides a feline serum albumin mutant Mut-FSA. By replacing the K at the 558th position at the end of the FSA amino acid sequence with R, the optimized Mut-FSA sequence is finally formed. Compared with the unmutated FSA, Mut-FSA can show more significant curative effect advantages in the treatment intervention of feline hypoproteinemia or acute blood loss. In addition, Mut-FSA can not only enable the serum albumin concentration of feline hypoproteinemia patients to be rapidly corrected within 24 hours after treatment intervention, but also effectively improve the serum albumin level and overall prognosis of the affected cats. The recombinant FSA prepared by the present invention can not only provide a more effective innovative therapy for feline hypoproteinemia or acute blood loss, but also has broad application prospects and industrialization value.

The invention discloses a traditional Chinese medicine compound preparation for treating nephrotic syndrome and a preparation method of the traditional Chinese medicine compound preparation, and belongs to the technical field of nephrotic syndrome treatment.The synbiotic microcapsule is prepared by compatibility of traditional Chinese medicines such as safflower, scutellaria baicalensis and perilla frutescens, and the synbiotic microcapsule is prepared by embedding traditional Chinese medicine extracts such as astragalus membranaceus and lily with lactobacillus acidophilus and bifidobacterium; and the wall material is prepared from mannose grafted meadowfoam seed extract, sodium alginate and fucoidin sulfate. The synbiotics microcapsule utilizes a Maillard reaction modified wall material to enhance gastric acid erosion resistance, realizes intestinal targeted colonization through mannose residues, protects probiotics, directionally releases traditional Chinese medicine active components, adjusts intestinal flora balance and inhibits uremia toxin generation. The traditional Chinese medicine components such as the safflower and the scutellaria baicalensis improve kidney blood circulation, relieve inflammatory response and regulate lipid metabolism by inhibiting an NF-kappa B channel, activating an AMPK channel and the like; the components such as the Chinese yam and the Chinese wolfberry can repair the permeability of glomerular basement membrane and improve the hypoproteinemia.

According to the albumin mRNA enteric capsule and the application thereof, an innovative treatment strategy of oral intestinal delivery of albumin mRNA based on an LNP freeze-dried capsule is proposed for the first time, an organism is induced to autonomously synthesize functional albumin, the scheme breaks through the limitation of a traditional infusion therapy, and meanwhile, the long-term biosafety risk of an mRNA therapy is avoided; a brand new treatment scheme and a treatment strategy are provided for the treatment of the hypoproteinemia.

The invention discloses a wheat flavone derivative derived from spartina alterniflora and a preparation method and application thereof. The wheat flavone derivative has a structural expression shown in the specification, the wheat flavone derivative provided by the invention not only can effectively reduce the blood uric acid level, but also can improve metabolic disorders related to hyperuricemia, such as hyperglycemia, hypercholesterol and hypoproteinemia, and has a multi-target effect; .

The present invention provides feline serum albumin, its isolation method, and applications, relating to the field of genetic engineering technology. This invention, for the first time, mutates feline serum albumin to obtain beneficial mutation sites, thereby producing a feline serum albumin mutant that can be highly expressed in a Pichia pastoris system. Furthermore, the feline serum albumin mutant L94H not only exhibits excellent antioxidant effects but also significantly increases albumin concentration in cats, offering promising applications in treating feline hypoproteinemia, cirrhosis, ascites, and other conditions, or alleviating liver damage.

The invention relates to a polyprotein peptide composition and application of the polyprotein peptide composition in the aspect of hypoproteinemia, and belongs to the field of biological medicine. The polyprotein peptide composition comprises the following components in parts by weight: 40-60 parts of ovalbumin peptide; 15 to 30 parts of fish collagen peptide; 10 to 20 parts of sea cucumber oligopeptide; 5 to 15 parts of cordyceps militaris oligopeptide; and 5 to 15 parts of yeast beta-glucan. The ovalbumin peptide is used as a core protein source, the fish collagen peptide, the sea cucumber oligopeptide, the cordyceps militaris oligopeptide and the yeast beta-glucan are compounded, the components achieve a synergistic effect according to a specific weight ratio, the A / G protein inversion state of a patient suffering from the hypoproteinemia can be rapidly improved, wound granulation tissue hyperplasia and healing are promoted, physical strength is enhanced, fatigue is resisted, and the curative effect is good. The composition can be used for treating hypoproteinemia, enhancing lymphocyte proliferation and NK cell activity, reducing side effects of chemoradiotherapy, improving the curative effect of anti-tumor treatment and remarkably improving the prognosis of patients, and can be widely applied to nutrition intervention, postoperative rehabilitation and tumor chemoradiotherapy adjuvant therapy of hypoproteinemia patients.

The invention relates to a bionic glomerular multistage sorting membrane and application thereof in continuous blood purification (CBP), and belongs to the technical field of biomedical engineering and blood purification. According to the membrane, the filtration mechanism of natural glomerulus is simulated, gradient aperture design and charge selective modification are adopted, efficient sorting is achieved, small molecules (such as urea and creatinine), middle molecules (such as beta2-microglobulin and inflammatory factors) and macromolecular toxins (such as protein binding toxins) can be removed at the same time, and meanwhile abnormal loss of albumin is greatly reduced. A traditional blood purification membrane is generally poor in treatment effect on solutes with different sizes and properties due to lack of a multi-stage sorting function, and especially has significant insufficiency in the aspect of albumin retention, so that the risks of hypoproteinemia and malnutrition of patients are increased. The bionic sorting membrane is closer to natural glomerulus in structure and function, the sorting and selective barrier characteristics are optimized, the bionic sorting membrane is particularly suitable for advanced blood purification systems such as CRRT and high interception hemodialysis (HCO-HD), an innovative extracorporeal circulation treatment solution is provided for critical patients, and the application prospect is wide. And the safety and the effect of overall treatment are improved.