Formula and preparation process of hydrolyzed protein liquid beverage

Abstract

The invention relates to the technical field of food beverages, in particular to a formula and a preparation process of a hydrolyzed protein liquid beverage, the formula of the hydrolyzed protein liquid beverage comprises protein, a supplement and a conditioning agent, the supplement is selected from one or more of calcium hydroxybutyrate, leucine, isoleucine and valine, and the conditioning agent is selected from one or more of calcium hydroxybutyrate, leucine, isoleucine and valine. The regulator is selected from one or more of gamma-aminobutyric acid, glycine, taurine, 5-hydroxytryptamine and beta-aminobutyric acid; peptide molecules contained in the hydrolyzed protein liquid beverage disclosed by the invention can quickly enter blood circulation through intestinal tracts; for a patient in a hypoproteinemia state before and after an operation, rapid and effective protein supplement is crucial, postoperative recovery is promoted, and the occurrence rate of complications is reduced; for traumatic injuries such as fracture, burn and the like, timely supplement of high-quality protein components is a key for accelerating wound healing and tissue repair.

Description

Technical Field

[0001] The invention relates to the technical field of food and beverage, and in particular to a formula and preparation process of a hydrolyzed protein liquid beverage. Background Art

[0002] With the deterioration of the environment and changes in eating habits, the incidence of cancer patients in my country has shown a rapid growth trend. For cancer patients, the level of protein in the blood is one of the key indicators for assessing their health status before and after surgery and during intensive care. At present, the main means to improve this indicator is through the infusion of human albumin solution. However, due to the high cost of human albumin, most patients can hardly afford the financial burden of long-term use. Therefore, during the postoperative recovery phase, patients often need to rely on effective nutritional support to promote the repair of damaged tissues, enhance the function of the immune system, and prolong survival.

[0003] At the same time, as the elderly age, various systems and organs of the human body gradually show signs of aging, which is mainly due to the loss of important components such as collagen in the body and the aging of the cells themselves.

[0004] In summary, the present invention provides an innovative solution that can both meet the postoperative recovery needs of tumor patients and improve the health of the elderly population.

[0005] Therefore, it is of great significance to develop a hydrolyzed protein liquid beverage that can not only meet the postoperative recovery needs of cancer patients but also improve the health of the elderly. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a formula and preparation process of a hydrolyzed protein liquid beverage.

[0007] In a first aspect, the present invention provides a hydrolyzed protein liquid beverage, comprising protein, a supplement and a regulator, wherein the supplement is selected from one or more of calcium hydroxybutyrate, leucine, isoleucine and valine, and the regulator is selected from one or more of γ-aminobutyric acid, glycine, taurine, 5-hydroxytryptamine and β-aminobutyric acid.

[0008] In the present invention, the supplement is preferably selected from calcium hydroxybutyrate, and the regulator is preferably selected from gamma-aminobutyric acid.

[0009] In the present invention, calcium hydroxybutyrate (calcium β-hydroxy β-methylbutyrate) has a certain sedative effect and is suitable for improving insomnia. It can produce a sense of relaxation by affecting neurotransmitters in the brain, helping people fall asleep better. As an efficient anabolic nutritional supplement, HMB calcium can stimulate the growth of muscle cells and increase body weight. In addition, it can also help restore neurological dysfunction caused by brain diseases, such as the recovery period after cerebral infarction or cerebral hemorrhage. Calcium hydroxybutyrate also helps to alleviate the situation of cerebral vascular sclerosis and promote the secretion of growth hormone; γ-aminobutyric acid (GABA) is an important inhibitory neurotransmitter in the central nervous system. Although it is not an amino acid that constitutes protein, it plays a vital physiological role in the body. GABA can activate the brain, promote intellectual development, fight epileptic seizures, improve sleep quality, maintain skin health, and slow down the aging process of the brain. It can also supplement the inhibitory neurotransmitters required by the human body and effectively lower blood pressure. In addition, GABA helps to improve kidney function, prevent fatty liver and obesity, activate liver function, and is beneficial to the stability of the heart, brain and blood pressure. At the same time, it can also promote the balance of amino acid metabolism in the body and regulate the function of the immune system.

[0010] Preferably, the protein is selected from one or more combinations of albumin, globulin, fibrinogen, whey protein powder, casein hydrolysate, and soy protein hydrolysate.

[0011] In the present invention, the protein is preferably a combination of albumin and whey protein powder.

[0012] In the present invention, the hydrolyzed whey protein powder is hydrolyzed, and its main components include dipeptides, tripeptides and eighteen kinds of amino acids, of which the proportion of essential amino acids is as high as 53%, and branched-chain amino acids account for 20%. The protein content of the product exceeds 80%, and the degree of hydrolysis is between 30% and 40%. In the hydrolyzed protein, oligopeptides with a molecular weight between 189 and 500 account for no less than 50%. This structure allows the product to be directly absorbed by the human body without digestion, and no residue will be left; albumin not only contributes to the growth and development of muscles, but also enhances the body's immune ability and memory function. In addition, albumin also contains an appropriate amount of fat, which helps maintain body temperature, protect internal organs, assist the absorption of vitamins in the body, and serve as an energy source. At the same time, albumin is rich in various vitamins, such as vitamins A, B2 and D, which can promote metabolism, maintain eye health, support bone growth and reduce the risk of osteoporosis. The amino acid combination in albumin is particularly suitable for human absorption and utilization.

[0013] Preferably, the mass ratio of the protein, the supplement and the regulator is (80-90):(5-10):(5-10).

[0014] Preferably, sweeteners, sour agents and enhancers are also included.

[0015] Preferably, the sweetener is selected from one of maltitol liquid, sorbitol and erythritol.

[0016] In the present invention, the sweetener is preferably selected from maltitol liquid.

[0017] Preferably, the acidulant is selected from one of citric acid, malic acid and lactic acid.

[0018] In the present invention, the acidulant is preferably selected from citric acid.

[0019] Preferably, the enhancer is selected from one of sucralose, aspartame, acesulfame potassium and steviol glycoside.

[0020] In the present invention, the enhancer is preferably selected from sucralose.

[0021] In the present invention, the mass ratio of the hydrolyzed protein liquid beverage ingredients is: hydrolyzed whey protein powder 15%-85%, albumin 5%-40%, β-hydroxy β-methylbutyrate calcium 1%-9%, citric acid 0.025%-2.8%, γ-aminobutyric acid 0.01%-1.5%, sucralose 0.015%-0.1%, and edible flavor 0.01%-0.1%.

[0022] In a second aspect, the present invention further provides a process for preparing a hydrolyzed protein liquid beverage, comprising the following steps:

[0023] S1. Weigh protein, supplements, regulators, sweeteners, acidulants and enhancers;

[0024] S2. Mix the protein, supplement and regulator evenly, add water and stir to dissolve;

[0025] S3, adding sweetener, acidulant and enhancer to the solution of S2, stirring and dissolving to obtain a crude product solution;

[0026] S4, filtering the crude product to obtain a refined product;

[0027] S5. Fill and disinfect the fine liquid, seal it, sterilize it, and then cool it to room temperature to obtain a finished product liquid, thereby preparing a hydrolyzed protein liquid beverage.

[0028] Preferably, in step S2, the stirring speed is 2000-3000 rpm / min.

[0029] Preferably, in step S3, the stirring speed is 3500-4000 rpm / min.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] (1) The peptide molecules contained in the hydrolyzed protein liquid beverage of the present invention can not only quickly enter the blood circulation through the intestines, but also maintain their biological activity in the body, thereby effectively participating in key life activities of the human body; for patients in a state of hypoproteinemia before and after surgery, rapid and effective protein supplementation is crucial, which helps to promote postoperative recovery and reduce the incidence of complications; for traumatic injuries such as fractures and burns, timely supplementation of high-quality protein components is the key to accelerating wound healing and tissue repair.

[0032] (2) The present invention uses albumin and whey protein powder as the main proteins. Both proteins are rich in essential amino acids and can effectively promote muscle recovery and development. They are particularly suitable for drinking after exercise. At the same time, the reasonable ratio of protein to supplements and regulators helps to improve the body's absorption and utilization rate of protein and achieve better nutritional supplementation. Calcium hydroxybutyrate, as an effective supplement, can promote bone health, increase bone density, and play a positive role in preventing osteoporosis. Gamma-aminobutyric acid is an important neurotransmitter that helps relieve stress, improve sleep quality, and plays an important role in improving the quality of life.

[0033] (3) The present invention not only reduces the caloric burden brought by traditional sugars, but also avoids the side effects that may be caused by artificial sweeteners by adding maltitol liquid as a sweetener. At the same time, the addition of citric acid can adjust the acidity of the beverage, making it taste more refreshing and natural. In addition, the use of sucralose as an enhancer can further enhance the overall flavor of the beverage while ensuring the sweetness, so that consumers can have a better drinking experience.

[0034] (4) The hydrolyzed protein beverage of the present invention is not only suitable for athletes or fitness enthusiasts to meet their needs for rapid recovery after high-intensity training; it is also suitable for people who need additional protein intake in daily life, such as the elderly, pregnant women, etc., to help improve physical condition and enhance immunity. DETAILED DESCRIPTION

[0035] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the protection scope of the present invention.

[0036] Example 1

[0037] This embodiment provides a preparation process of a hydrolyzed protein liquid beverage, comprising the following steps:

[0038] S1. Weigh 60 g of albumin, 20 g of whey protein powder, 1 mg of calcium hydroxybutyrate, 10 mg of γ-aminobutyric acid, 5 ml of maltitol solution, 0.05 ml of citric acid, 0.05 mg of sucralose, and 0.1 mg of edible flavor;

[0039] S2. Mix albumin, whey protein powder, calcium hydroxybutyrate and γ-aminobutyric acid evenly, add water and stir to dissolve, with the stirring speed of 2500 rpm / min;

[0040] S3, adding maltitol solution, citric acid and sucralose to the solution of S2, stirring to dissolve, and obtaining a crude product solution, the stirring speed is 3700 rpm / min;

[0041] S4, filtering the crude product to obtain a refined product;

[0042] S5. Fill and disinfect the fine liquid, seal it, sterilize it, and then cool it to room temperature to obtain a finished product liquid, thereby preparing a hydrolyzed protein liquid beverage.

[0043] Example 2

[0044] Compared with Example 1, 80 g of albumin, 40 g of whey protein powder, 1.5 mg of calcium hydroxybutyrate, 50 mg of γ-aminobutyric acid, 10 ml of maltitol solution, 0.05 ml of citric acid and 0.05 mg of sucralose, and 0.1 mg of edible flavor are used, and the remaining steps are the same as in Example 1.

[0045] Example 3

[0046] Compared with Example 1, 65 g of albumin, 25 g of whey protein powder, 1.2 mg of calcium hydroxybutyrate, 15 mg of γ-aminobutyric acid, 6 ml of maltitol solution, 0.05 ml of citric acid and 0.05 mg of sucralose, and 0.1 mg of edible flavor are used, and the remaining steps are the same as in Example 1.

[0047] Example 4

[0048] Compared with Example 1, 70 g of albumin, 30 g of whey protein powder, 1.3 mg of calcium hydroxybutyrate, 20 mg of γ-aminobutyric acid, 7 ml of maltitol solution, 0.05 ml of citric acid and 0.05 mg of sucralose, and 0.1 mg of edible flavor are used, and the remaining steps are the same as in Example 1.

[0049] Example 5

[0050] Compared with Example 1, 75 g of albumin, 35 g of whey protein powder, 1.4 mg of calcium hydroxybutyrate, 45 mg of γ-aminobutyric acid, 8 ml of maltitol solution, 0.05 ml of citric acid, 0.05 mg of sucralose, and 0.1 mg of edible flavor are used, and the remaining steps are the same as in Example 1.

[0051] Comparative Example 1

[0052] Compared with Example 3, albumin was not added, and the remaining steps were the same as Example 3.

[0053] Comparative Example 2

[0054] Compared with Example 3, whey protein powder was not added, and the remaining steps were the same as Example 3.

[0055] Comparative Example 3

[0056] Compared with Example 3, calcium hydroxybutyrate was not added, and the remaining steps were the same as Example 3.

[0057] Comparative Example 4

[0058] Compared with Example 3, γ-aminobutyric acid was not added, and the remaining steps were the same as Example 3.

[0059] Comparative Example 5

[0060] Compared with Example 3, albumin and whey protein powder are replaced with pea protein, and the remaining steps are the same as Example 3.

[0061] Comparative Example 6

[0062] Compared with Example 3, albumin and whey protein powder are replaced with casein, and the remaining steps are the same as Example 3.

[0063] Comparative Example 7

[0064] Compared with Example 3, γ-aminobutyric acid is replaced by γ-hydroxy-γ-methylbutyrate calcium, and the remaining steps are the same as Example 3.

[0065] Comparative Example 8

[0066] Compared with Example 3, calcium hydroxybutyrate is replaced by β-aminobutyric acid, and the remaining steps are the same as Example 3.

[0067] Comparative Example 9

[0068] Compared with Example 3, calcium hydroxybutyrate is replaced by α-aminobutyric acid, and the remaining steps are the same as Example 3.

[0069] Antioxidant activity: The DPPH free radical scavenging method was used to test the free radical scavenging rate of the hydrolyzed protein liquid beverage.

[0070] Protein content: The protein content of hydrolyzed protein liquid beverages was tested using the Kjeldahl method;

[0071] Bioactive Peptides: The content of specific bioactive peptides in the hydrolyzed protein liquid beverage was tested using a specific enzyme-linked immunosorbent assay (ELISA).

[0072] The performance of Examples 1-5 and Comparative Examples 1-9 was tested, and the test results are shown in Table 1.

[0073] Table 1

[0074]

[0075] From the results in Table 1, we can see that:

[0076] Embodiment: The free radical scavenging rate is relatively high, ranging from 94.2% to 96.4%; Comparative Example: The free radical scavenging rate is relatively low, ranging from 70.4% to 90.4%;

[0077] Example: The protein content is relatively high, ranging from 18.6% to 20.1%; Comparative Example: The protein content is relatively low, ranging from 13.5% to 17.8%;

[0078] Example: The antioxidant peptide content is relatively high, ranging from 201 μg / mL to 214 μg / mL; Comparative Example: The antioxidant peptide content is relatively low, ranging from 112 μg / mL to 175 μg / mL;

[0079] Albumin is a highly effective antioxidant that can significantly improve the free radical scavenging rate and antioxidant peptide content of beverages. The presence of albumin helps to improve the hydrolysis efficiency of proteins, thereby generating more antioxidant peptides; whey protein powder is rich in essential amino acids and antioxidant peptide precursors, which can increase protein content and the generation of antioxidant peptides. The addition of whey protein powder helps to improve the overall antioxidant capacity and nutritional value of beverages; calcium hydroxybutyrate is a metabolic intermediate that can provide energy and improve cell metabolism. Although it is not an antioxidant itself, it can indirectly increase the generation of antioxidant peptides and free radical scavenging rate by improving cell metabolism; γ-aminobutyric acid (GABA) is an inhibitory neurotransmitter with certain antioxidant and anti-inflammatory effects. The addition of GABA can enhance the antioxidant capacity of beverages and may increase the generation of antioxidant peptides by regulating cell metabolism; as can be seen from the data in Table 1, the hydrolyzed protein liquid beverage in the embodiment is significantly higher than the comparative example in terms of free radical scavenging rate, protein content and antioxidant peptide content. This suggests that adding ingredients such as albumin, whey protein powder, calcium hydroxybutyrate and gamma-aminobutyric acid can significantly improve the antioxidant capacity and nutritional value of beverages.

[0080] The specific embodiments further describe the present invention, but it should be understood that the specific description here should not be construed as limiting the essence and scope of the present invention. Various modifications made to the above embodiments by ordinary technicians in this field after reading this specification are all within the scope of protection of the present invention.

Claims

1. A hydrolyzed protein beverage, characterized in that: The invention comprises protein, supplement and regulator, wherein the supplement is selected from one or more of calcium hydroxybutyrate, leucine, isoleucine and valine, and the regulator is selected from one or more of gamma-aminobutyric acid, glycine, taurine, 5-hydroxytryptamine and beta-aminobutyric acid.

2. The protein hydrolyzate beverage according to claim 1, characterized in that: The protein is selected from one or more combinations of albumin, globulin, fibrinogen, whey protein powder, casein hydrolysate, and soy protein hydrolysate.

3. The protein hydrolyzate beverage according to claim 1, characterized in that: The mass ratio of the protein, the supplement and the regulator is (80-90):(5-10):(5-10).

4. The protein hydrolyzate beverage according to claim 1, characterized in that: Also included are sweeteners, sour agents and enhancers.

5. The protein hydrolyzate beverage according to claim 4, characterized in that: The sweetener is selected from one of maltitol liquid, sorbitol and erythritol.

6. The protein hydrolyzate beverage according to claim 4, characterized in that: The acidulant is selected from one of citric acid, malic acid and lactic acid.

7. The protein hydrolyzate beverage according to claim 4, characterized in that: The enhancer is selected from one of sucralose, aspartame, acesulfame potassium and steviol glycoside.

8. A process for preparing a hydrolyzed protein beverage according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Weigh protein, supplements, regulators, sweeteners, acidulants and enhancers; S2. Mix the protein, supplement and regulator evenly, add water and stir to dissolve; S3, adding sweetener, acidulant and enhancer to the solution of S2, stirring and dissolving to obtain a crude product solution; S4, filtering the crude product to obtain a refined product; S5. Fill and disinfect the fine liquid, seal it, sterilize it, and then cool it to room temperature to obtain a finished product liquid, thereby preparing a hydrolyzed protein liquid beverage.

9. The process for preparing the hydrolyzed protein beverage according to claim 8, characterized in that: In step S2, the stirring speed is 2000-3000 rpm / min.

10. The process for preparing the hydrolyzed protein beverage according to claim 8, characterized in that: In step S3, the stirring speed is 3500-4000 rpm / min.

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